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Literature summary extracted from

  • Thomas, J.L.; Duax, W.L.; Addlagatta, A.; Scaccia, L.A.; Frizzell, K.A.; Carloni, S.B.
    Serine 124 completes the Tyr, Lys and Ser triad responsible for the catalysis of human type 1 3beta-hydroxysteroid dehydrogenase (2004), J. Mol. Endocrinol., 33, 253-261.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.145 expression in Sf9 insect cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.145 S123A 24% of wild-type 3beta-HSD1 activity Homo sapiens
1.1.1.145 S124A no detectable 3beta-HSD1 activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.145 0.0037
-
 dehydroepiandrosterone 27°C, pH 7.4 Homo sapiens
1.1.1.145 0.011
-
 dehydroepiandrosterone 27°C, pH 7.4, S123A mutant 3betaHSD1 Homo sapiens
1.1.1.145 0.034
-
 NAD+ 27°C, pH 7.4 Homo sapiens
1.1.1.145 0.036
-
 NAD+ 27°C, pH 7.4, S123A mutant 3betaHSD1 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.145 dehydroepiandrosterone + NAD+ Homo sapiens
-
 androst-5-en-3,17-dione + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.145 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.145 recombinant 3beta-HSD1 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.145 placenta
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.145 dehydroepiandrosterone + NAD+
-
 Homo sapiens androst-5-en-3,17-dione + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.145 3beta-hydroxysteroid dehydrogenase type 1
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.145 0.055
-
 dehydroepiandrosterone 27°C, pH 7.4 Homo sapiens
1.1.1.145 0.06
-
 NAD+ 27°C, pH 7.4 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.145 9.7
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.145 7.4 9.7 37% of maximal activity at pH 7.4 Homo sapiens