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Literature summary extracted from

  • Kato, S.i.; Ohshima, T.; Galkin, A.; Kulakova, L.; Yoshimura, T.; Esaki, N.
    Purification and characterization of alanine dehydrogenase from a marine bacterium,Vibrio proteolyticus (2003), J. Mol. Catal. B, 23, 373-378.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.1 expression in Escherichia coli TG1 with a vector plasmid, pUC118 Vibrio proteolyticus

General Stability

EC Number General Stability Organism
1.4.1.1 RbCl and NaCl, significantly stabilize the enzyme. LiCl and KCl stabilize to a lower degree Vibrio proteolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.1 Cu2+
-
 Vibrio proteolyticus
1.4.1.1 HgCl2 1 mM, complete inhibition Vibrio proteolyticus
1.4.1.1 methyl p-nitrobenzenesulfonate 0.1 mM, complete inhibition Vibrio proteolyticus
1.4.1.1 Pb2+
-
 Vibrio proteolyticus
1.4.1.1 PCMB 0.1 mM, 97% inhibition Vibrio proteolyticus
1.4.1.1 Sn2+
-
 Vibrio proteolyticus
1.4.1.1 SO42-
-
 Vibrio proteolyticus
1.4.1.1 Zn2+
-
 Vibrio proteolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.1 0.09
-
 NADH 25°C Vibrio proteolyticus
1.4.1.1 0.18
-
 NAD+ 25°C Vibrio proteolyticus
1.4.1.1 0.61
-
 pyruvate 25°C Vibrio proteolyticus
1.4.1.1 30
-
 L-Ala 25°C Vibrio proteolyticus
1.4.1.1 110
-
 NH3 25°C Vibrio proteolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.1 40000
-
 6 * 40000, SDS-PAGE Vibrio proteolyticus
1.4.1.1 230000
-
 gel filtration Vibrio proteolyticus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Vibrio proteolyticus O85596 DSM 30189
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.1
-
 Vibrio proteolyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.1 150
-
-
 Vibrio proteolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 3-fluoropyruvate + NH3 + NADH 74% of the activity with pyruvate Vibrio proteolyticus ? + H2O + NAD+
-
 ?
1.4.1.1 3-hydroxypyruvate + NH3 + NADH 54% of the activity with pyruvate Vibrio proteolyticus L-Ser + H2O + NAD+
-
 ?
1.4.1.1 pyruvate + NH3 + NADH
-
 Vibrio proteolyticus L-Ala + H2O + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.1 hexamer 6 * 40000, SDS-PAGE Vibrio proteolyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.1 50
-
 pH 7.2, 60 min, stable Vibrio proteolyticus
1.4.1.1 55
-
 pH 7.2, 60 min, 70% loss of activity Vibrio proteolyticus
1.4.1.1 65
-
 60 min in presence of 2.0 M NaCl, enzyme retains more than 90% of its activity, 98% of its activity is lost in absence of NaCl Vibrio proteolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.1 8
-
 reductive amination of pyruvate Vibrio proteolyticus
1.4.1.1 10 10.3 oxidative deamination of L-Ala Vibrio proteolyticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.1.1 6 9.5 25°C, 10 min, no effect Vibrio proteolyticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.1 NAD+ no activity with NADP+ Vibrio proteolyticus
1.4.1.1 NADH no activity with NADPH Vibrio proteolyticus