EC Number | General Stability | Organism |
---|---|---|
1.4.1.9 | enzyme requires a high concentration of salt for stability | Natrialba magadii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.9 | AgNO3 | 1 mM, complete | Natrialba magadii | |
1.4.1.9 | HgCl2 | 1 mM, complete | Natrialba magadii | |
1.4.1.9 | NADH | competitive inhibition versus NAD+, noncompetitive inhibition with respect to L-Leu | Natrialba magadii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.1.9 | 0.02 | - |
NADH | pH 9.5, 30°C | Natrialba magadii | |
1.4.1.9 | 0.48 | - |
NAD+ | pH 9.5, 30°C | Natrialba magadii | |
1.4.1.9 | 0.66 | - |
L-Ile | pH 9.5, 30°C, in presence of 4 M NaCL | Natrialba magadii | |
1.4.1.9 | 0.69 | - |
L-Leu | pH 9.5, 30°C | Natrialba magadii | |
1.4.1.9 | 0.71 | - |
L-Val | pH 9.5, 30°C, in presence of 4 M NaCL | Natrialba magadii | |
1.4.1.9 | 4 | - |
2-ketoisocaproate | pH 9.5, 30°C | Natrialba magadii | |
1.4.1.9 | 220 | - |
NH3 | pH 9.5, 30°C | Natrialba magadii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.9 | CsCl | enzyme shows maximal activity in presence of more than 3 M salt | Natrialba magadii | |
1.4.1.9 | KCl | enzyme shows maximal activity in presence of more than 3 M salt | Natrialba magadii | |
1.4.1.9 | NaCl | enzyme shows maximal activity in presence of more than 3 M salt | Natrialba magadii | |
1.4.1.9 | RbCl | enzyme shows maximal activity in presence of more than 4 M salt | Natrialba magadii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.1.9 | 55000 | - |
6 * 55000, at 2.5 M NaCl, dissociates into trimers and dimers in a lower concentration of salts, cetyltrimethylammonium-bromide-PAGE | Natrialba magadii |
1.4.1.9 | 330000 | - |
gel filtration | Natrialba magadii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.9 | Natrialba magadii | - |
- |
- |
1.4.1.9 | Natrialba magadii MS-3 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.1.9 | - |
Natrialba magadii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.1.9 | 1.92 | - |
- |
Natrialba magadii |
EC Number | Storage Stability | Organism |
---|---|---|
1.4.1.9 | 30°C, 2 months, 50% loss of activity in presence of 2.5 M NaCl | Natrialba magadii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.9 | 2-ketobutyrate + NH3 + NADH | 70% of the activity with 2-ketoisocaproate | Natrialba magadii | L-2-aminobutyrate + H2O + NAD+ | - |
? | |
1.4.1.9 | 2-ketobutyrate + NH3 + NADH | 70% of the activity with 2-ketoisocaproate | Natrialba magadii MS-3 | L-2-aminobutyrate + H2O + NAD+ | - |
? | |
1.4.1.9 | 2-ketocaproate + NH3 + NADH | 69% of the activity with 2-ketoisocaproate | Natrialba magadii | L-2-aminohexanoate + H2O + NAD+ | - |
? | |
1.4.1.9 | 2-ketoisocaproate + NH3 + NADH | - |
Natrialba magadii | L-Ile + H2O + NAD+ | - |
? | |
1.4.1.9 | 2-ketoisovalerate + NH3 + NADH | 150% of the activity with 2-ketoisocaproate | Natrialba magadii | L-Val + H2O + NAD+ | - |
? | |
1.4.1.9 | 2-ketovalerate + NH3 + NADH | 96% of the activity with 2-ketoisocaproate | Natrialba magadii | L-norvaline + H2O + NAD+ | - |
? | |
1.4.1.9 | L-2-aminobutyrate + H2O + NAD+ | 32% of the activity with L-Leu | Natrialba magadii | 2-oxobutanoate + NH3 + NADH | - |
? | |
1.4.1.9 | L-Ile + H2O + NAD+ | 72% of the activity with L-Leu | Natrialba magadii | 3-methyl-2-oxopentanoate + NH3 + NADH | - |
? | |
1.4.1.9 | L-Ile + H2O + NAD+ | 72% of the activity with L-Leu | Natrialba magadii MS-3 | 3-methyl-2-oxopentanoate + NH3 + NADH | - |
? | |
1.4.1.9 | L-Leu + H2O + NAD+ | - |
Natrialba magadii | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
? | |
1.4.1.9 | L-Leu + H2O + NAD+ | - |
Natrialba magadii MS-3 | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
? | |
1.4.1.9 | L-norvaline + H2O + NAD+ | 7% the activity with L-Leu | Natrialba magadii | 2-oxopentanoate + NH3 + NADH | - |
? | |
1.4.1.9 | L-norvaline + H2O + NAD+ | 7% the activity with L-Leu | Natrialba magadii MS-3 | 2-oxopentanoate + NH3 + NADH | - |
? | |
1.4.1.9 | L-Val + H2O + NAD+ | 59% of the activity with L-Leu | Natrialba magadii | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
? | |
1.4.1.9 | L-Val + H2O + NAD+ | 59% of the activity with L-Leu | Natrialba magadii MS-3 | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
? | |
1.4.1.9 | S-methyl-L-cysteine + H2O + NAD+ | 19% the activity with L-Leu | Natrialba magadii | 3-methylthio-2-oxopropanoate + NH3 + NADH | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.1.9 | hexamer | 6 * 55000, at 2.5 M NaCl, dissociates into trimers and dimers in a lower concentration of salts, cetyltrimethylammonium-bromide-PAGE | Natrialba magadii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.1.9 | 65 | - |
- |
Natrialba magadii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.1.9 | 30 | - |
2 months, 50% loss of activity in presence of 2.5 M NaCl | Natrialba magadii |
1.4.1.9 | 50 | - |
1 h, stable in presence of 2.5 M NaCl or 2.5 M KCl | Natrialba magadii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.9 | 9.5 | - |
oxidative deamination of L-Leu and reductive amination of 2-ketoisocaproate, in presence of 3 M NaCl | Natrialba magadii |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.4.1.9 | 8 | 10 | pH 8: about 30% of maximal activity, pH 10: about 70% of maximal activity, reductive amination of 2-ketoisocaproate | Natrialba magadii |
1.4.1.9 | 9 | 10.5 | pH 9: about 40% of maximal activity, pH 10.5: about 55% of maximal activity, oxidative deamination of L-Leu | Natrialba magadii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.9 | NAD+ | - |
Natrialba magadii | |
1.4.1.9 | NADH | - |
Natrialba magadii |