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Literature summary extracted from

  • Martino, A.; Schiraldi, C.; Fusco, S.; Di Lernia, I.; Costabile, T.; Pellicano, T.; Marotta, M.; Generoso, M.; van der Oost, J.; Sensen, C.W.; Charlebois, R.L.; Moracci, M.; Rossi, M.; De Rosa, M.
    Properties of the recombinant alpha-glucosidase from Sulfolobus solfataricus in relation to starch processing (2001), J. Mol. Catal. B, 11, 787-794.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.20 nutrition usage in industrial processes to hydrolyze starch, saccharification step Saccharolobus solfataricus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.20 gene SsGA, DNA sequence determination and analysis, expression in Escherichia coli BL21(DE3), cytosolic recombinant enzyme Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Saccharolobus solfataricus
-
-
-
3.2.1.20 Saccharolobus solfataricus MT-4 / DSM 5833
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 recombinant enzyme from Escherichia coli by selective and progressive thermal precipitation, 14.5fold to homogeneity Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 6.66
-
 purified recombinant enzyme, substrate maltohexaose Saccharolobus solfataricus
3.2.1.20 6.95
-
 purified recombinant enzyme, substrate maltotriose Saccharolobus solfataricus
3.2.1.20 18.8
-
 purified recombinant enzyme, substrate 4-nitrophenyl alpha-D-glucopyranoside Saccharolobus solfataricus
3.2.1.20 35.6
-
 purified recombinant enzyme, substrate maltose Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.20 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Saccharolobus solfataricus MT-4 / DSM 5833 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.20 maltohexaose + H2O
-
 Saccharolobus solfataricus maltopentaose + D-glucose
-
 ?
3.2.1.20 maltohexaose + H2O
-
 Saccharolobus solfataricus MT-4 / DSM 5833 maltopentaose + D-glucose
-
 ?
3.2.1.20 maltose + H2O
-
 Saccharolobus solfataricus 2 D-glucose
-
 ?
3.2.1.20 maltose + H2O
-
 Saccharolobus solfataricus MT-4 / DSM 5833 2 D-glucose
-
 ?
3.2.1.20 maltotriose + H2O
-
 Saccharolobus solfataricus maltose + D-glucose
-
 ?
3.2.1.20 maltotriose + H2O
-
 Saccharolobus solfataricus MT-4 / DSM 5833 maltose + D-glucose
-
 ?
3.2.1.20 additional information no activity with starch and amylose Saccharolobus solfataricus ?
-
 ?
3.2.1.20 additional information no activity with starch and amylose Saccharolobus solfataricus MT-4 / DSM 5833 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 100
-
-
 Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.20 75
-
 50% remaining activity after 40-70 h, half-life: 156 h Saccharolobus solfataricus
3.2.1.20 85
-
 completely stable up to for 2 h, half-life: 150 h Saccharolobus solfataricus
3.2.1.20 90
-
 loss of 21.2% activity within 2 h Saccharolobus solfataricus
3.2.1.20 100
-
 loss of 55.2% activity within 2 h, half-life: 80 min Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 5
-
-
 Saccharolobus solfataricus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.20 3.5 8.5 completely stable Saccharolobus solfataricus