Literature summary extracted from
Moroz, O.V.; Harkiolaki, M.; Galperin, M.Y.; Vagin, A.A.; Gonzalez-Pacanowska, D.; Wilson, K.S.
The crystal structure of a complex of Campylobacter jejuni dUTPase with substrate analogue sheds light on the mechanism and suggests the "basic module" for dimeric d(C/U)TPases (2004), J. Mol. Biol., 342, 1583-1597.
Application
EC Number |
Application |
Comment |
Organism |
---|
3.6.1.23 |
medicine |
potential drug target |
Campylobacter jejuni |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.6.1.23 |
expressed in Escherichia coli BL21(DE3) |
Campylobacter jejuni |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.6.1.23 |
- |
Campylobacter jejuni |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.6.1.23 |
Mg2+ |
required, three ions per subunit |
Campylobacter jejuni |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.6.1.23 |
dUTP + H2O |
Campylobacter jejuni |
removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA |
dUMP + diphosphate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.6.1.23 |
Campylobacter jejuni |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.6.1.23 |
recombinant protein |
Campylobacter jejuni |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.6.1.23 |
dUTP + H2O |
- |
Campylobacter jejuni |
dUMP + diphosphate |
- |
? |
|
3.6.1.23 |
dUTP + H2O |
removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA |
Campylobacter jejuni |
dUMP + diphosphate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.6.1.23 |
dimer |
crystal structure analysis |
Campylobacter jejuni |