Literature summary extracted from

Nichols, C.E.; Dhaliwal, B.; Lockyer, M.; Hawkins, A.R.; Stammers, D.K.
High-resolution structures reveal details of domain closure and "half-of-sites-reactivity" in Escherichia coli aspartate beta-semialdehyde dehydrogenase (2004), J. Mol. Biol., 341, 797-806.

Application

EC Number	Application	Comment	Organism
1.2.1.11	medicine	enzyme is potential target for antimicrobial drugs	Escherichia coli

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.2.1.11	recombinant large scale expression in strain BL21(DE3)	Escherichia coli

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
1.2.1.11	enzyme in open and closed form, 30 mg/ml purified recombinant enzyme in 10 mM Tris, pH 7.4, 40 mM KCl, with equal volume of reservoir solution, 0.006 ml sitting drops by vapour diffusion utilizing micro-bridges, 20% v/v ethylene glycol, 4°C, X-ray diffraction structure determination and analysis at 1.95 A and 1.6 A resolution, respectively, modeling	Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.2.1.11	L-4-aspartyl phosphate + NADPH	Escherichia coli	part of the biosynthetic aspartate pathway	L-aspartate-4-semialdehyde + phosphate + NADP+	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.2.1.11	Escherichia coli	P0A9Q9	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.2.1.11	recombinant enzyme from strain BL21(DE3) in several steps	Escherichia coli

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.2.1.11	L-4-aspartyl phosphate + NADPH	part of the biosynthetic aspartate pathway	Escherichia coli	L-aspartate-4-semialdehyde + phosphate + NADP+	-	?
1.2.1.11	L-4-aspartyl phosphate + NADPH	reductive dephosphorylation	Escherichia coli	L-aspartate-4-semialdehyde + phosphate + NADP+	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.2.1.11	dimer	crystal structure, subdomain structure of subunits, open and closed enzyme forms	Escherichia coli

Synonyms

EC Number	Synonyms	Comment	Organism
1.2.1.11	aspartate beta-semialdehyde dehydrogenase	-	Escherichia coli
1.2.1.11	ecASADH	-	Escherichia coli

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.2.1.11	NADPH	-	Escherichia coli

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Release 2023.1 (January 2023)