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Literature summary extracted from

  • Tanaka, N.; Kusakabe, Y.; Ito, K.; Yoshimoto, T.; Nakamura, K.T.
    Crystal structure of formaldehyde dehydrogenase from Pseudomonas putida: the structural origin of the tightly bound cofactor in nicotinoprotein dehydrogenases (2002), J. Mol. Biol., 324, 519-533.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.46 hanging drop vapor diffusion method Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.46 Zn2+ two ions bound per subunit Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.46 42000
-
 4 * 42000 Pseudomonas putida
1.2.1.46 170000
-
-
 Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.46 formaldehyde + NAD+ + H2O Pseudomonas putida removal of toxic formaldehyde formate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.46 Pseudomonas putida P46154
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.46 formaldehyde + NAD+ + H2O
-
 Pseudomonas putida formate + NADH
-
 ?
1.2.1.46 formaldehyde + NAD+ + H2O removal of toxic formaldehyde Pseudomonas putida formate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.46 tetramer 4 * 42000 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.46 NAD+ tightly but not covalently bound to protein, one molecule bound per subunit Pseudomonas putida