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Literature summary extracted from

  • Bae, J.D.; Cho, Y.J.; Kim, D.I.; Lee, D.S.; Shin, H.J.
    Purification and biochemical characterization of recombinant alanine dehydrogenase from Thermus caldophilus GK24 (2003), J. Microbiol. Biotechnol., 13, 628-631.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.1 CHCl3 20% Thermus caldophilus
1.4.1.1 hexane 20% Thermus caldophilus
1.4.1.1 Zn2+ 1 mM Thermus caldophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.1 0.2
-
 pyruvate
-
 Thermus caldophilus
1.4.1.1 1.8
-
 NAD+
-
 Thermus caldophilus
1.4.1.1 2.6
-
 L-Ala
-
 Thermus caldophilus
1.4.1.1 39.4
-
 NH4+
-
 Thermus caldophilus
1.4.1.1 50
-
 NADH
-
 Thermus caldophilus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Thermus caldophilus
-
-
-
1.4.1.1 Thermus caldophilus GK24
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.1 recombinant enzyme Thermus caldophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 L-Ala + H2O + NAD+
-
 Thermus caldophilus pyruvate + NH3 + NADH
-
 r
1.4.1.1 L-Ala + H2O + NAD+
-
 Thermus caldophilus GK24 pyruvate + NH3 + NADH
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.1 70
-
 stable Thermus caldophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.1 8
-
 reductive amination of pyruvate Thermus caldophilus
1.4.1.1 12
-
 oxidative deamination of L-Ala Thermus caldophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.1 NAD+
-
 Thermus caldophilus
1.4.1.1 NADH
-
 Thermus caldophilus