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Literature summary extracted from

  • Kushi, R.T.; Monti, R.; Contiero, J.
    Production, purification and characterization of an extracellular inulinase from Kluyveromyces marxianus var. bulgaricus (2000), J. Ind. Microbiol. Biotechnol., 25, 63-69.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.7 additional information
-
 additional information 4.58 mg/ml, for sucrose Kluyveromyces marxianus
3.2.1.7 additional information
-
 additional information 7.41 mg/ml, for raffinose Kluyveromyces marxianus
3.2.1.7 additional information
-
 additional information 86.9 mg/ml, for inulin Kluyveromyces marxianus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.7 extracellular
-
 Kluyveromyces marxianus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.7 57000
-
 SDS-PAGE Kluyveromyces marxianus
3.2.1.7 77000
-
 gel filtration on Superose column Kluyveromyces marxianus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.7 Kluyveromyces marxianus
-
 var. bulgaricus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.7 by DEAE-Trisacryl Plus column and Superose column Kluyveromyces marxianus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.7 inulin + H2O
-
 Kluyveromyces marxianus beta-D-fructose + inulobiose + fructooligosaccharides
-
 ?
3.2.1.7 raffinose + H2O
-
 Kluyveromyces marxianus D-fructose + D-galactopyranosyl-(1-6)-D-glucopyranoside
-
 ?
3.2.1.7 sucrose + H2O
-
 Kluyveromyces marxianus beta-D-fructose + D-glucose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.7 55
-
-
 Kluyveromyces marxianus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.7 4.7
-
 for sucrose Kluyveromyces marxianus