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Literature summary extracted from

  • Bravo, D.T.; Harris, D.O.; Parsons, S.M.
    Reliable, sensitive, rapid and quantitative enzyme-based assay for gamma-hydroxybutyric acid (GHB) (2004), J. Forensic Sci., 49, 379-387.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.61 analysis dipstick assay for the detection of gamma-hydroxybutyrate in alcoholic beverages Cupriavidus necator

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.61 expressed in Escherichia coli pLysS as stable fusion protein Cupriavidus necator

General Stability

EC Number General Stability Organism
1.1.1.61 addition of ammonium sulfate, glycerol, 2-mercaptoethanol, dithiothreitol, glutathione, or EDTA reduces activity during storage Cupriavidus necator

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.61 2.19
-
 gamma-hydroxybutyrate pH 8.5, 23°C Cupriavidus necator
1.1.1.61 14
-
 ethanol pH 8.5, 23°C Cupriavidus necator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.61 40500
-
 calculated from the predicted amino acid sequence of the native enzyme Cupriavidus necator
1.1.1.61 66000
-
 fusion protein, SDS-PAGE, calculated mass Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.61 4-hydroxybutanoate + NADP+ Cupriavidus necator
-
 succinate semialdehyde + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.61 Cupriavidus necator
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.61
-
 Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.61 4-hydroxybutanoate + NADP+
-
 Cupriavidus necator succinate semialdehyde + NADPH + H+
-
 ?
1.1.1.61 ethanol + NADP+
-
 Cupriavidus necator ethanal + NADPH + H+
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.61 -80
-
 stable for indefinite timespan Cupriavidus necator
1.1.1.61 4
-
 stable for at least 21 days, 60% of activity after 3.5 months Cupriavidus necator