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Literature summary extracted from
- Glycosylasparaginase inhibition studies: competitive inhibitors, transition state mimics, noncompetitive inhibitors (2001), J. Enzyme Inhib., 16, 269-274.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.26 | additional information | intramolecular autoproteolytic activation reaction | Homo sapiens |
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.5.1.26 | medicine | enzyme deficiency or its absence gives rise to aspartylglycosaminuria, an autosomal recessive inherited disorder that results in the accumulation of glycoasparagines in lysosomes, most common disorder of glycoprotein metabolism | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.26 | 3-phosphono-DL-2-aminopropionic acid | phosphono transition state mimic, competitive inhibitor | Homo sapiens |  |
| 3.5.1.26 | L-2-bromosuccinic acid | competitive inhibitor | Homo sapiens | |
| 3.5.1.26 | L-2-chlorosuccinic acid | competitive inhibitor | Homo sapiens | |
| 3.5.1.26 | L-2-methylsuccinic acid | competitive inhibitor | Homo sapiens | |
| 3.5.1.26 | L-aspartic acid | competitive inhibitor | Homo sapiens | |
| 3.5.1.26 | L-cysteic acid | sulfo transition state mimic, competitive inhibitor | Homo sapiens | |
| 3.5.1.26 | additional information | not inhibited by L-cysteine | Homo sapiens | |
| 3.5.1.26 | N-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)bromoacetamide | substrate analogue, noncompetitive inhibitor | Homo sapiens | |
| 3.5.1.26 | N-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)chloroacetamide | substrate analogue, noncompetitive inhibitor | Homo sapiens | |
| 3.5.1.26 | N1-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)glycinamide | substrate analogue, noncompetitive inhibitor | Homo sapiens | |
| 3.5.1.26 | succinic acid | competitive inhibitor | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.26 | 0.116 | - |
N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.26 | additional information | Homo sapiens | enzyme catalyzes the hydrolysis of the N-glycosylic bond between asparagine and N-acetylglucosamine in the catabolism of N-linked glycoproteins | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.26 | Homo sapiens | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.5.1.26 | proteolytic modification | intramolecular autoproteolytic activation reaction | Homo sapiens |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.5.1.26 | N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O = N-acetyl-beta-D-glucosaminylamine + L-aspartate | mechanism | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.1.26 | amniotic fluid | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.26 | additional information | enzyme catalyzes the hydrolysis of the N-glycosylic bond between asparagine and N-acetylglucosamine in the catabolism of N-linked glycoproteins | Homo sapiens | ? | - |
? | |
| 3.5.1.26 | N4-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O | enzyme catalyzes the hydrolysis of the N-glycosylic bond between asparagine and N-acetylglucosamine, mechanism involving formation of a tetra-hedral high-energy intermediate, presence of a second binding site that may recognize substituted acetamido groups | Homo sapiens | N-acetyl-beta-D-glucosaminylamine + L-aspartate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.26 | dimer | - |
Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.26 | GA | - |
Homo sapiens |
| 3.5.1.26 | glycosylasparaginase | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.26 | 37 | - |
assay at | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.26 | 5.8 | - |
assay at | Homo sapiens |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.26 | 0.56 | - |
N-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)bromoacetamide | pH 5.8, 37°C | Homo sapiens | |
| 3.5.1.26 | 0.6 | - |
L-aspartic acid | pH 5.8, 37°C | Homo sapiens | |
| 3.5.1.26 | 0.64 | - |
N-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)chloroacetamide | pH 5.8, 37°C | Homo sapiens | |
| 3.5.1.26 | 0.7 | - |
L-2-methylsuccinic acid | pH 5.8, 37°C | Homo sapiens | |
| 3.5.1.26 | 0.75 | - |
N1-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)glycinamide | pH 5.8, 37°C | Homo sapiens | |
| 3.5.1.26 | 0.9 | - |
3-phosphono-DL-2-aminopropionic acid | pH 5.8, 37°C | Homo sapiens | |
| 3.5.1.26 | 1.4 | - |
L-cysteic acid | pH 5.8, 37°C | Homo sapiens | |
| 3.5.1.26 | 2.7 | - |
L-2-bromosuccinic acid | pH 5.8, 37°C | Homo sapiens | |
| 3.5.1.26 | 5 | - |
succinic acid | pH 5.8, 37°C | Homo sapiens | |
| 3.5.1.26 | 7.7 | - |
L-2-chlorosuccinic acid | pH 5.8, 37°C | Homo sapiens | |
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