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Literature summary extracted from

  • Kimura, S.; Iyama, S.; Yamaguchi, Y.; Kanakura, Y.
    New enzymatic assay for serum urea nitrogen using urea amidolyase (2003), J. Clin. Lab. Anal., 17, 52-56.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.4.6 ATP ATP-dependent cleavage of urea Saccharomyces cerevisiae

Application

EC Number Application Comment Organism
6.3.4.6 diagnostics enzymatic assay for measurement of serum urea nitrogen using urea amidolyase Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.6 Ca2+ strong, Ca2+ affects the enzyme-ATP-Mg complex by the time of urea carboxylation Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.6 additional information
-
 additional information
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.6 HCO3- requirement, hydrolysis of urea Saccharomyces cerevisiae
6.3.4.6 K+ requirement, hydrolysis of urea Saccharomyces cerevisiae
6.3.4.6 Mg2+ requirement, hydrolysis of urea Saccharomyces cerevisiae
6.3.4.6 additional information requires divalent and monovalent ions Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.6 Saccharomyces cerevisiae P32528
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.4.6 ATP + urea + HCO3- + H+ = ADP + phosphate + urea-1-carboxylate a biotinyl-protein, the yeast enzyme (but not that from green algae) also catalyses the reaction of EC 3.5.1.54 allophanate hydrolase, thus bringing about the hydrolysis of urea to CO2 and NH3, previously also listed as EC 3.5.1.45 Saccharomyces cerevisiae
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.6 ATP + urea + CO2 multifunctional enzyme, catalyzes carboxylation of urea with urea carboxylase, EC 6.3.4.6, and hydrolysis of allophanate with allophanate hydrolase, EC 3.5.1.54 Saccharomyces cerevisiae ADP + phosphate + urea-1-carboxylate
-
 ?
6.3.4.6 urea + H2O + ATP in the presence of enzyme, ATP, bicarbonate, magnesium, and potassium ions, ammonium ion is produced from human serum urea Saccharomyces cerevisiae CO2 + NH3 + ADP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.4.6 ATP-urea amidolyase
-
 Saccharomyces cerevisiae
6.3.4.6 urea amidolyase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.4.6 37
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.6 7.5 8 hydrolysis of urea Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.4.6 additional information
-
 additional information
-
 Saccharomyces cerevisiae