Literature summary extracted from
Kimura, S.; Iyama, S.; Yamaguchi, Y.; Kanakura, Y.
New enzymatic assay for serum urea nitrogen using urea amidolyase (2003), J. Clin. Lab. Anal., 17, 52-56.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
6.3.4.6 |
ATP |
ATP-dependent cleavage of urea |
Saccharomyces cerevisiae |
|
Application
EC Number |
Application |
Comment |
Organism |
---|
6.3.4.6 |
diagnostics |
enzymatic assay for measurement of serum urea nitrogen using urea amidolyase |
Saccharomyces cerevisiae |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
6.3.4.6 |
Ca2+ |
strong, Ca2+ affects the enzyme-ATP-Mg complex by the time of urea carboxylation |
Saccharomyces cerevisiae |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
6.3.4.6 |
additional information |
- |
additional information |
- |
Saccharomyces cerevisiae |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
6.3.4.6 |
HCO3- |
requirement, hydrolysis of urea |
Saccharomyces cerevisiae |
|
6.3.4.6 |
K+ |
requirement, hydrolysis of urea |
Saccharomyces cerevisiae |
|
6.3.4.6 |
Mg2+ |
requirement, hydrolysis of urea |
Saccharomyces cerevisiae |
|
6.3.4.6 |
additional information |
requires divalent and monovalent ions |
Saccharomyces cerevisiae |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
6.3.4.6 |
Saccharomyces cerevisiae |
P32528 |
- |
- |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
6.3.4.6 |
ATP + urea + HCO3- + H+ = ADP + phosphate + urea-1-carboxylate |
a biotinyl-protein, the yeast enzyme (but not that from green algae) also catalyses the reaction of EC 3.5.1.54 allophanate hydrolase, thus bringing about the hydrolysis of urea to CO2 and NH3, previously also listed as EC 3.5.1.45 |
Saccharomyces cerevisiae |
|
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
6.3.4.6 |
ATP + urea + CO2 |
multifunctional enzyme, catalyzes carboxylation of urea with urea carboxylase, EC 6.3.4.6, and hydrolysis of allophanate with allophanate hydrolase, EC 3.5.1.54 |
Saccharomyces cerevisiae |
ADP + phosphate + urea-1-carboxylate |
- |
? |
|
6.3.4.6 |
urea + H2O + ATP |
in the presence of enzyme, ATP, bicarbonate, magnesium, and potassium ions, ammonium ion is produced from human serum urea |
Saccharomyces cerevisiae |
CO2 + NH3 + ADP + phosphate |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
6.3.4.6 |
ATP-urea amidolyase |
- |
Saccharomyces cerevisiae |
6.3.4.6 |
urea amidolyase |
- |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
6.3.4.6 |
37 |
- |
assay at |
Saccharomyces cerevisiae |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
6.3.4.6 |
7.5 |
8 |
hydrolysis of urea |
Saccharomyces cerevisiae |
Ki Value [mM]
EC Number |
Ki Value [mM] |
Ki Value maximum [mM] |
Inhibitor |
Comment |
Organism |
Structure |
---|
6.3.4.6 |
additional information |
- |
additional information |
- |
Saccharomyces cerevisiae |
|