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Literature summary extracted from
- A thermostable alpha-arabinofuranosidase from xylanolytic Bacillus pumilus: purification and characterization (2003), J. Biotechnol., 101, 69-79.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.55 | 1.7 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 7.0, 37°C | Bacillus pumilus |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.55 | extracellular | secretion to the cell culture medium containing arabinogalactan or oat meal | Bacillus pumilus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.55 | 220000 | - |
gel filtration | Bacillus pumilus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.55 | Bacillus pumilus | - |
xylanolytic bacterium, strain PS213 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.55 | to homogeneity from cell culture supernatant | Bacillus pumilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.55 | 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Bacillus pumilus | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.55 | tetramer | 4 * 56000-60000, SDS-PAGE | Bacillus pumilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.55 | 55 | - |
- |
Bacillus pumilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.55 | 65 | - |
loss of 80% activity after 2 h | Bacillus pumilus |
| 3.2.1.55 | 75 | - |
50% loss of activity after 135 min | Bacillus pumilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.55 | 7 | - |
- |
Bacillus pumilus |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.55 | Bacillus pumilus | - |
- |
4.9 |
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