BRENDA - Enzyme Database

A thermostable histamine oxidase from Arthrobacter crystallopoietes KAIT-B-007

Sekiguchi, Y.; Makita, H.; Yamamura, A.; Matsumoto, K.; J. Biosci. Bioeng. 97, 104-110 (2004)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.3.22
1,10-phenanthroline
10 mM, 47% inhibition
Arthrobacter crystallopoietes
1.4.3.22
2-mercaptoethanol
10 mM, 26% inhibition
Arthrobacter crystallopoietes
1.4.3.22
8-hydroxyquinoline
10 mM, 24% inhibition
Arthrobacter crystallopoietes
1.4.3.22
alpha,alpha'-dipyridyl
10 mM, 22% inhibition
Arthrobacter crystallopoietes
1.4.3.22
Diethyl dithiocarbamate
10 mM, 76% inhibition
Arthrobacter crystallopoietes
1.4.3.22
dithiothreitol
10 mM, 12% inhibition
Arthrobacter crystallopoietes
1.4.3.22
EDTA
10 mM, 18% inhibition
Arthrobacter crystallopoietes
1.4.3.22
hydrazine
10 mM, 95% inhibition
Arthrobacter crystallopoietes
1.4.3.22
Iproniazide
10 mM, 43% inhibition
Arthrobacter crystallopoietes
1.4.3.22
isoniazide
10 mM, 76% inhibition
Arthrobacter crystallopoietes
1.4.3.22
methylhydrazine
10 mM, complete inhibition
Arthrobacter crystallopoietes
1.4.3.22
N,N-diethyldithiocarbamate
the inactive enzyme is restored with Cu2+ to 65% of its initial activity
Arthrobacter crystallopoietes
1.4.3.22
phenylhydrazine
10 mM, complete inhibition
Arthrobacter crystallopoietes
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.3.22
0.51
-
histamine
pH 7.0, 37°C
Arthrobacter crystallopoietes
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.4.3.22
copper
one atom of copper per mol of enzyme protein
Arthrobacter crystallopoietes
1.4.3.22
Cu2+
a part of the apoenzyme is reconstituted in presence of Cu2+
Arthrobacter crystallopoietes
1.4.3.22
MgSO4
10 mM, activation to 114% of control
Arthrobacter crystallopoietes
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.3.22
81000
-
gel filtration, native PAGE
Arthrobacter crystallopoietes
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.4.3.22
Arthrobacter crystallopoietes
-
KAIT-B-007
-
1.4.3.22
Arthrobacter crystallopoietes KAIT-B-007
-
KAIT-B-007
-
Purification (Commentary)
EC Number
Commentary
Organism
1.4.3.22
-
Arthrobacter crystallopoietes
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4.3.22
14.3
-
-
Arthrobacter crystallopoietes
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.3.22
1-phenylethylamine + H2O + O2
44% of the activity with histamine
656323
Arthrobacter crystallopoietes
1-phenylethanal + NH3 + H2O2
-
-
-
?
1.4.3.22
1-phenylethylamine + H2O + O2
44% of the activity with histamine
656323
Arthrobacter crystallopoietes KAIT-B-007
1-phenylethanal + NH3 + H2O2
-
-
-
?
1.4.3.22
agmatine + H2O + O2
4% of the activity with histamine
656323
Arthrobacter crystallopoietes
?
-
-
-
?
1.4.3.22
agmatine + H2O + O2
4% of the activity with histamine
656323
Arthrobacter crystallopoietes KAIT-B-007
?
-
-
-
?
1.4.3.22
butylamine + H2O + O2
22% of the activity with histamine
656323
Arthrobacter crystallopoietes
butanal + NH3 + H2O2
-
-
-
?
1.4.3.22
dopamine + H2O + O2
51% of the activity with histamine
656323
Arthrobacter crystallopoietes
2-(3,4-dihydroxyphenyl)ethanal + NH3 + H2O2
-
-
-
?
1.4.3.22
hexylamine + H2O + O2
41% of the activity with histamine
656323
Arthrobacter crystallopoietes
hexanal + NH3 + H2O2
-
-
-
?
1.4.3.22
histamine + H2O + O2
-
656323
Arthrobacter crystallopoietes
(imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
-
-
?
1.4.3.22
histamine + H2O + O2
-
656323
Arthrobacter crystallopoietes KAIT-B-007
(imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
-
-
?
1.4.3.22
additional information
no activity with putrescine, cadaverine, spermine, and spermidine
656323
Arthrobacter crystallopoietes
?
-
-
-
-
1.4.3.22
additional information
no activity with putrescine, cadaverine, spermine, and spermidine
656323
Arthrobacter crystallopoietes KAIT-B-007
?
-
-
-
-
1.4.3.22
pentylamine + H2O + O2
32% of the activity with histamine
656323
Arthrobacter crystallopoietes
pentanal + NH3 + H2O2
-
-
-
?
1.4.3.22
propylamine + H2O + O2
7% of the activity with histamine
656323
Arthrobacter crystallopoietes
propanal + NH3 + H2O2
-
-
-
?
1.4.3.22
tryptamine + H2O + O2
15% of the activity with histamine
656323
Arthrobacter crystallopoietes
1H-indol-3-ylacetaldehyde + NH3 + H2O2
-
-
-
?
1.4.3.22
tyramine + H2O + O2
36% of the activity with histamine
656323
Arthrobacter crystallopoietes
4-hydroxyphenylethanal + NH3 + H2O2
-
-
-
?
1.4.3.22
tyramine + H2O + O2
36% of the activity with histamine
656323
Arthrobacter crystallopoietes KAIT-B-007
4-hydroxyphenylethanal + NH3 + H2O2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.4.3.22
monomer
1 * 81000, SDS-PAGE
Arthrobacter crystallopoietes
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.4.3.22
65
-
10 min, stable
Arthrobacter crystallopoietes
1.4.3.22
70
-
10 min, 93% inactivation
Arthrobacter crystallopoietes
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.3.22
9
-
-
Arthrobacter crystallopoietes
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.4.3.22
6
9
37°C, 30 min, stable
Arthrobacter crystallopoietes
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.4.3.22
1,10-phenanthroline
10 mM, 47% inhibition
Arthrobacter crystallopoietes
1.4.3.22
2-mercaptoethanol
10 mM, 26% inhibition
Arthrobacter crystallopoietes
1.4.3.22
8-hydroxyquinoline
10 mM, 24% inhibition
Arthrobacter crystallopoietes
1.4.3.22
alpha,alpha'-dipyridyl
10 mM, 22% inhibition
Arthrobacter crystallopoietes
1.4.3.22
Diethyl dithiocarbamate
10 mM, 76% inhibition
Arthrobacter crystallopoietes
1.4.3.22
dithiothreitol
10 mM, 12% inhibition
Arthrobacter crystallopoietes
1.4.3.22
EDTA
10 mM, 18% inhibition
Arthrobacter crystallopoietes
1.4.3.22
hydrazine
10 mM, 95% inhibition
Arthrobacter crystallopoietes
1.4.3.22
Iproniazide
10 mM, 43% inhibition
Arthrobacter crystallopoietes
1.4.3.22
isoniazide
10 mM, 76% inhibition
Arthrobacter crystallopoietes
1.4.3.22
methylhydrazine
10 mM, complete inhibition
Arthrobacter crystallopoietes
1.4.3.22
N,N-diethyldithiocarbamate
the inactive enzyme is restored with Cu2+ to 65% of its initial activity
Arthrobacter crystallopoietes
1.4.3.22
phenylhydrazine
10 mM, complete inhibition
Arthrobacter crystallopoietes
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.3.22
0.51
-
histamine
pH 7.0, 37°C
Arthrobacter crystallopoietes
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.4.3.22
copper
one atom of copper per mol of enzyme protein
Arthrobacter crystallopoietes
1.4.3.22
Cu2+
a part of the apoenzyme is reconstituted in presence of Cu2+
Arthrobacter crystallopoietes
1.4.3.22
MgSO4
10 mM, activation to 114% of control
Arthrobacter crystallopoietes
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.4.3.22
81000
-
gel filtration, native PAGE
Arthrobacter crystallopoietes
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.4.3.22
-
Arthrobacter crystallopoietes
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4.3.22
14.3
-
-
Arthrobacter crystallopoietes
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.3.22
1-phenylethylamine + H2O + O2
44% of the activity with histamine
656323
Arthrobacter crystallopoietes
1-phenylethanal + NH3 + H2O2
-
-
-
?
1.4.3.22
1-phenylethylamine + H2O + O2
44% of the activity with histamine
656323
Arthrobacter crystallopoietes KAIT-B-007
1-phenylethanal + NH3 + H2O2
-
-
-
?
1.4.3.22
agmatine + H2O + O2
4% of the activity with histamine
656323
Arthrobacter crystallopoietes
?
-
-
-
?
1.4.3.22
agmatine + H2O + O2
4% of the activity with histamine
656323
Arthrobacter crystallopoietes KAIT-B-007
?
-
-
-
?
1.4.3.22
butylamine + H2O + O2
22% of the activity with histamine
656323
Arthrobacter crystallopoietes
butanal + NH3 + H2O2
-
-
-
?
1.4.3.22
dopamine + H2O + O2
51% of the activity with histamine
656323
Arthrobacter crystallopoietes
2-(3,4-dihydroxyphenyl)ethanal + NH3 + H2O2
-
-
-
?
1.4.3.22
hexylamine + H2O + O2
41% of the activity with histamine
656323
Arthrobacter crystallopoietes
hexanal + NH3 + H2O2
-
-
-
?
1.4.3.22
histamine + H2O + O2
-
656323
Arthrobacter crystallopoietes
(imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
-
-
?
1.4.3.22
histamine + H2O + O2
-
656323
Arthrobacter crystallopoietes KAIT-B-007
(imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
-
-
?
1.4.3.22
additional information
no activity with putrescine, cadaverine, spermine, and spermidine
656323
Arthrobacter crystallopoietes
?
-
-
-
-
1.4.3.22
additional information
no activity with putrescine, cadaverine, spermine, and spermidine
656323
Arthrobacter crystallopoietes KAIT-B-007
?
-
-
-
-
1.4.3.22
pentylamine + H2O + O2
32% of the activity with histamine
656323
Arthrobacter crystallopoietes
pentanal + NH3 + H2O2
-
-
-
?
1.4.3.22
propylamine + H2O + O2
7% of the activity with histamine
656323
Arthrobacter crystallopoietes
propanal + NH3 + H2O2
-
-
-
?
1.4.3.22
tryptamine + H2O + O2
15% of the activity with histamine
656323
Arthrobacter crystallopoietes
1H-indol-3-ylacetaldehyde + NH3 + H2O2
-
-
-
?
1.4.3.22
tyramine + H2O + O2
36% of the activity with histamine
656323
Arthrobacter crystallopoietes
4-hydroxyphenylethanal + NH3 + H2O2
-
-
-
?
1.4.3.22
tyramine + H2O + O2
36% of the activity with histamine
656323
Arthrobacter crystallopoietes KAIT-B-007
4-hydroxyphenylethanal + NH3 + H2O2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.4.3.22
monomer
1 * 81000, SDS-PAGE
Arthrobacter crystallopoietes
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.4.3.22
65
-
10 min, stable
Arthrobacter crystallopoietes
1.4.3.22
70
-
10 min, 93% inactivation
Arthrobacter crystallopoietes
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.4.3.22
9
-
-
Arthrobacter crystallopoietes
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.4.3.22
6
9
37°C, 30 min, stable
Arthrobacter crystallopoietes