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Literature summary extracted from

  • Isobe, K.; Mori, N.; Wakao, N.
    The second cholesterol oxidase produced by gamma-Proteobacterium Y-134 (2003), J. Biosci. Bioeng., 96, 257-261.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.3.6 stable in nonionic detergents over a wide range. More than 90% of activity remains after 60 min at 60°C, at concentrations of 0.1% and 0.5% of emulgen A-60, Triton X-100, Triton X-405 or sodium cholate gamma proteobacterium Y-134

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.6 2-pyridylhydroxymethane sulfate 1 mM, 30 min at 4°C, 32% inhibition gamma proteobacterium Y-134
1.1.3.6 8-hydroxyquinoline 1 mM, 30 min at 4°C, 20% inhibition gamma proteobacterium Y-134
1.1.3.6 CoCl2 1 mM, 30 min at 4°C, 21% inhibition gamma proteobacterium Y-134
1.1.3.6 CuCl2 1 mM, 30 min at 4°C, 29% inhibition gamma proteobacterium Y-134
1.1.3.6 FAD 1 mM, 30 min at 4°C, 21% inhibition gamma proteobacterium Y-134
1.1.3.6 FMN 1 mM, 30 min at 4°C, 21% inhibition gamma proteobacterium Y-134
1.1.3.6 iodoacetic acid 1 mM, 30 min at 4°C, 15% inhibition gamma proteobacterium Y-134
1.1.3.6 MgCl2 1 mM, 30 min at 4°C, 23% inhibition gamma proteobacterium Y-134
1.1.3.6 MnCl2 1 mM, 30 min at 4°C, 35% inhibition gamma proteobacterium Y-134
1.1.3.6 NEM 1 mM, 30 min at 4°C, 24% inhibition gamma proteobacterium Y-134
1.1.3.6 o-phenanthroline 1 mM, 30 min at 4°C, 26% inhibition gamma proteobacterium Y-134
1.1.3.6 potassium cyanide 1 mM, 30 min at 4°C, 15% inhibition gamma proteobacterium Y-134
1.1.3.6 riboflavin 1 mM, 30 min at 4°C, 20% inhibition gamma proteobacterium Y-134
1.1.3.6 rotenone 1 mM, 30 min at 4°C, 12% inhibition gamma proteobacterium Y-134

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.6 0.026
-
 cholesterol pH 6.0 or pH 7.0 gamma proteobacterium Y-134

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.6 58000
-
 gel filtration gamma proteobacterium Y-134

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.6 gamma proteobacterium Y-134
-
 uncultured
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.6 enzyme CHO-U gamma proteobacterium Y-134

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.6 39.6
-
-
 gamma proteobacterium Y-134

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.6 7-dehydrocholesterol + O2 26% of the activity with cholesterol gamma proteobacterium Y-134 cholest-4,7-dien-3-one + H2O2
-
 ?
1.1.3.6 beta-sitosterol + O2 19% of the activity with cholesterol gamma proteobacterium Y-134 stigmast-5-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 gamma proteobacterium Y-134 cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 dihydrocholesterol + O2 22% of the activity with cholesterol gamma proteobacterium Y-134 5alpha-cholestan-3-one + H2O2
-
 ?
1.1.3.6 additional information less than 3% of the activity with cholesterol: ergosterol and epiandrosterone. No activity: dehydroepiandrosterone, pregnenolone gamma proteobacterium Y-134 ?
-
 ?
1.1.3.6 stigmastanol + O2 7% of the activity with cholesterol gamma proteobacterium Y-134 stigmastanone + H2O2
-
 ?
1.1.3.6 stigmasterol + O2 15% of the activity with cholesterol gamma proteobacterium Y-134 stigmasta-5,22-dien-3-one + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.6 monomer 1 * 58000, SDS-PAGE gamma proteobacterium Y-134

Synonyms

EC Number Synonyms Comment Organism
1.1.3.6 CHO-U
-
 gamma proteobacterium Y-134

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.6 60
-
 pH 6.0, 7 h, approximately 65% loss of activity of CHO-U gamma proteobacterium Y-134
1.1.3.6 70
-
 pH 6.0, 1 h, 40% loss of activity of CHO-U gamma proteobacterium Y-134

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.6 5.5 6
-
 gamma proteobacterium Y-134

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.6 5 6.5 pH 5.0: about 90% of maximal activity, pH 6.5: about 60% of maximal activity, pH 7.0: about 20% of maximal activity gamma proteobacterium Y-134

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.6 5 8.5 60 min, more than 80% of the CHO-U activity remains gamma proteobacterium Y-134

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.6 FAD
-
 gamma proteobacterium Y-134

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.3.6 gamma proteobacterium Y-134 isoelectric fucusing, pH-gradient 3.5-10.0
-
 7