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Literature summary extracted from

  • Kim, Y.J.; Yoshizawa, M.; Takenaka, S.; Murakami, S.; Aoki, K.
    Ammonia assimilation in Klebsiella pneumoniae F-5-2 that can utilize ammonium and nitrate ions simultaneously: purification and characterization of glutamate dehydrogenase and glutamine synthetase (2002), J. Biosci. Bioeng., 93, 584-588.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.4 AgNO3 1 mM, 30°C, complete loss of aminating activity Klebsiella pneumoniae
1.4.1.4 AlCl3 1 mM, 30°C, 23% loss of aminating activity Klebsiella pneumoniae
1.4.1.4 BaCl2 1 mM, 30°C, 15% loss of aminating activity Klebsiella pneumoniae
1.4.1.4 CaCl2 1 mM, 30°C, 11% loss of aminating activity Klebsiella pneumoniae
1.4.1.4 CH2ICOOH 1 mM, 30°C, 15% loss of aminating activity Klebsiella pneumoniae
1.4.1.4 FeSO3(NH4)2SO4 1 mM, 30°C, 14% loss of aminating activity Klebsiella pneumoniae
1.4.1.4 HgCl2 1 mM, 30°C, 48% loss of aminating activity Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.4 52000
-
 6 * 52000, SDS-PAGE Klebsiella pneumoniae
1.4.1.4 300000
-
 gel filtration Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.4 2-oxoglutarate + NADPH + NH3 Klebsiella pneumoniae ammonia-assimilating enzyme L-glutamate + NADP+ + H2O
-
 r
1.4.1.4 2-oxoglutarate + NADPH + NH3 Klebsiella pneumoniae F-5-2 ammonia-assimilating enzyme L-glutamate + NADP+ + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.4 Klebsiella pneumoniae
-
-
-
1.4.1.4 Klebsiella pneumoniae F-5-2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.4
-
 Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.4 190
-
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.4 2-oxoadipate + NADPH + NH3 6.3% of the activity with 2-oxoglutarate Klebsiella pneumoniae L-2-aminoadipate + NADP+ + H2O
-
 ?
1.4.1.4 2-oxoadipate + NADPH + NH3 6.3% of the activity with 2-oxoglutarate Klebsiella pneumoniae F-5-2 L-2-aminoadipate + NADP+ + H2O
-
 ?
1.4.1.4 2-oxobutyrate + NADPH + NH3 12% of the activity with 2-oxoglutarate Klebsiella pneumoniae L-2-aminobutyrate + NADP+ + H2O
-
 ?
1.4.1.4 2-oxobutyrate + NADPH + NH3 12% of the activity with 2-oxoglutarate Klebsiella pneumoniae F-5-2 L-2-aminobutyrate + NADP+ + H2O
-
 ?
1.4.1.4 2-oxoglutarate + NADPH + NH3
-
 Klebsiella pneumoniae L-glutamate + NADP+ + H2O
-
 r
1.4.1.4 2-oxoglutarate + NADPH + NH3 ammonia-assimilating enzyme Klebsiella pneumoniae L-glutamate + NADP+ + H2O
-
 r
1.4.1.4 2-oxoglutarate + NADPH + NH3
-
 Klebsiella pneumoniae F-5-2 L-glutamate + NADP+ + H2O
-
 r
1.4.1.4 2-oxoglutarate + NADPH + NH3 ammonia-assimilating enzyme Klebsiella pneumoniae F-5-2 L-glutamate + NADP+ + H2O
-
 r
1.4.1.4 2-oxovalerate + NADPH + NH3 15% of the activity with 2-oxoglutarate Klebsiella pneumoniae L-2-aminovalerate + NADP+ + H2O
-
 ?
1.4.1.4 additional information no activity with 2-oxoisovalerate and pyruvate Klebsiella pneumoniae ?
-
 ?
1.4.1.4 additional information no activity with 2-oxoisovalerate and pyruvate Klebsiella pneumoniae F-5-2 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.4 hexamer 6 * 52000, SDS-PAGE Klebsiella pneumoniae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.4 50
-
 pH 8.0, 10 min, maintains more than 90% activity Klebsiella pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.4 8
-
 aminating activity Klebsiella pneumoniae
1.4.1.4 9 10 deaminating activity Klebsiella pneumoniae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.1.4 5.5 11.5 stable Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.4 NADP+
-
 Klebsiella pneumoniae
1.4.1.4 NADPH
-
 Klebsiella pneumoniae