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Literature summary extracted from

  • Kitamoto, N.; Yoshino-Yasuda, S.; Ohmiya, K.; Tsukagoshi, N.
    A second pectin lyase gene (pel2) from Aspergillus oryzae KBN616: its sequence analysis and overexpression, and characterization of the gene products (2001), J. Biosci. Bioeng., 91, 378-381.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.10 gene pel2, DNA and amino acid sequence determination and analysis, enzyme contains a signal peptide of 19 amino acids, overexpression under control of the promotor of the Aspergillus oryzae TEF1 gene Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.10 44000
-
 x * 44000, nonglycosylated recombinant enzyme, x * 48000, glycosylated recombinant enzyme Aspergillus oryzae
4.2.2.10 48000
-
 x * 44000, nonglycosylated recombinant enzyme, x * 48000, glycosylated recombinant enzyme Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.10 Aspergillus oryzae
-
 shoyu koji mold, strain KBN616, second gene pel2
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.2.10 glycoprotein
-
 Aspergillus oryzae

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.10 recombinant overexpressed enzyme Aspergillus oryzae

Subunits

EC Number Subunits Comment Organism
4.2.2.10 ? x * 44000, nonglycosylated recombinant enzyme, x * 48000, glycosylated recombinant enzyme Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
4.2.2.10 pectin lyase
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.10 50
-
 glycosylated and nonglycosylated enzyme Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.10 6
-
 glycosylated and nonglycosylated enzyme Aspergillus oryzae