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Literature summary extracted from

  • Ueda, S.; Oda, M.; Imamura, S.; Ohnishi, M.
    Steady-state kinetic properties of 3alpha-hydroxysteroid dehydrogenase from Pseudomonas sp. B-0831: Steroid substrate specificity and nucleotide cofactor dependency (2004), J. Biol. Macromol., 4, 23-28.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.50 additional information
-
 additional information steady-state kinetics Pseudomonas sp.
1.1.1.50 0.0062
-
 Fusidic acid pH 8.5, 37°C, with cofactor NAD+ Pseudomonas sp.
1.1.1.50 0.031
-
 cholic acid pH 8.5, 37°C, with cofactor NAD+ Pseudomonas sp.
1.1.1.50 0.037
-
 Fusidic acid pH 8.5, 37°C, with cofactor thio-NAD+ Pseudomonas sp.
1.1.1.50 0.038
-
 glycocholic acid pH 8.5, 37°C, with cofactor NAD+ Pseudomonas sp.
1.1.1.50 0.043
-
 taurocholic acid pH 8.5, 37°C, with cofactor NAD+ Pseudomonas sp.
1.1.1.50 0.063
-
 chenodeoxycholic acid pH 8.5, 37°C, with cofactor NAD+ Pseudomonas sp.
1.1.1.50 0.068
-
 lithocholic acid pH 8.5, 37°C, with cofactor NAD+ Pseudomonas sp.
1.1.1.50 0.069
-
 (3alpha,5beta,7beta)-3,7-dihydroxycholan-24-oic acid pH 8.5, 37°C, with cofactor NAD+ Pseudomonas sp.
1.1.1.50 0.084
-
 cholic acid pH 8.5, 37°C, with cofactor thio-NAD+ Pseudomonas sp.
1.1.1.50 0.21
-
 androsterone pH 8.5, 37°C, with cofactor NAD+ Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.50 Pseudomonas sp.
-
 srain B-0831
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.50 androsterone + NAD+
-
 Pseudomonas sp. 5alpha-androstane-3,17-dione + NADH
-
 ?
1.1.1.50 chenodeoxycholic acid + NAD+
-
 Pseudomonas sp. 7alpha,12alpha-dihydroxy-5beta-cholan-3-one-24-oic acid + NADH
-
 ?
1.1.1.50 cholic acid + NAD+
-
 Pseudomonas sp. 7alpha,12alpha-dihydroxy-5beta-cholan-3-one-24-oic acid + NADH
-
 ?
1.1.1.50 cholic acid + thio-NAD+
-
 Pseudomonas sp. 7alpha,12alpha-dihydroxy-5beta-cholan-3-one-24-oic acid + thio-NADH
-
 ?
1.1.1.50 fusidic acid + NAD+ antibiotic steroid substrate Pseudomonas sp. 3-oxofusidic acid + NADH
-
 ?
1.1.1.50 glycocholic acid + NAD+
-
 Pseudomonas sp. 7alpha,12alpha-dihydroxy-5beta-cholan-3-one-24-oyl-glycine + NADH
-
 ?
1.1.1.50 lithocholic acid + NAD+
-
 Pseudomonas sp. 5beta-cholan-3-one-24-oic acid + NADH
-
 ?
1.1.1.50 additional information broad steroid substrate and cofactor specificity, no 3-oxosteroid reduction of testosterone Pseudomonas sp. ?
-
 ?
1.1.1.50 taurocholic acid + NAD+
-
 Pseudomonas sp. 7alpha,12alpha-dihydroxy-5beta-cholan-3-one-24-oyl-taurine + NADH
-
 ?
1.1.1.50 ursodeoxycholic acid + NAD+
-
 Pseudomonas sp. 7alpha,12alpha-dihydroxy-5beta-cholan-3-one-24-oic acid + NADH
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.50 3alpha-hydroxysteroid dehydrogenase
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.50 37
-
 assay at Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.50 23
-
 Fusidic acid pH 8.5, 37°C, with cofactor NAD+ Pseudomonas sp.
1.1.1.50 25
-
 Fusidic acid pH 8.5, 37°C, with cofactor thio-NAD+ Pseudomonas sp.
1.1.1.50 35
-
 cholic acid pH 8.5, 37°C, with cofactor thio-NAD+ Pseudomonas sp.
1.1.1.50 72
-
 lithocholic acid pH 8.5, 37°C, with cofactor NAD+ Pseudomonas sp.
1.1.1.50 74
-
 taurocholic acid pH 8.5, 37°C, with cofactor NAD+ Pseudomonas sp.
1.1.1.50 75
-
 cholic acid pH 8.5, 37°C, with cofactor NAD+ Pseudomonas sp.
1.1.1.50 76
-
 chenodeoxycholic acid pH 8.5, 37°C, with cofactor NAD+ Pseudomonas sp.
1.1.1.50 77
-
 glycocholic acid pH 8.5, 37°C, with cofactor NAD+ Pseudomonas sp.
1.1.1.50 82
-
 (3alpha,5beta,7beta)-3,7-dihydroxycholan-24-oic acid pH 8.5, 37°C, with cofactor NAD+ Pseudomonas sp.
1.1.1.50 134
-
 androsterone pH 8.5, 37°C, with cofactor NAD+ Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.50 8.5
-
 assay at Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.50 3-acetylpyridine adenine dinucleotide i.e. APAD+, 42% of the activity with NAD+ when cholic acid is utilized as substrate Pseudomonas sp.
1.1.1.50 additional information cofactor specificity with different substrates Pseudomonas sp.
1.1.1.50 NAD+ best cofactor, preferably utilized with substrate cholic acid Pseudomonas sp.
1.1.1.50 nicotinamide hypoxanthine dinucleotide i.e. deamino-NAD+, 36% of the activity with NAD+ when cholic acid is utilized as substrate Pseudomonas sp.
1.1.1.50 thio-NAD+ 47% of the activity with NAD+ when cholic acid is utilized as substrate, preferably utilized with substrate fusidic acid Pseudomonas sp.