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Literature summary extracted from

  • Hagedoorn, P.L.; Chen, T.; Schroder, I.; Piersma, S.R.; Vries, S.D.; Hagen, W.R.
    Purification and characterization of the tungsten enzyme aldehyde:ferredoxin oxidoreductase from the hyperthermophilic denitrifier Pyrobaculum aerophilum (2005), J. Biol. Inorg. Chem., 10, 259-269.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.5 0.3
-
crotonaldehyde pH 8.4, 60°C Pyrobaculum aerophilum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.5 Iron contains 3.3 mol Fe per mol of monomer, the enzyme contains a single [4Fe-4S]2+/1+ cluster per monomer Pyrobaculum aerophilum
1.2.7.5 additional information contains no detectable molybdenum Pyrobaculum aerophilum
1.2.7.5 Tungsten contains 0.7 mol tungsten per mol of monomer, the enzyme contains a single tungstopterin cofactor Pyrobaculum aerophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.5 68000
-
2 * 68000, SDS-PAGE Pyrobaculum aerophilum
1.2.7.5 130000
-
gel filtration Pyrobaculum aerophilum

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.5 Pyrobaculum aerophilum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.5
-
Pyrobaculum aerophilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.5 6.5
-
-
Pyrobaculum aerophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.5 acetaldehyde + H2O + oxidized benzyl viologen at 5% of the activity with crotonaldehyde Pyrobaculum aerophilum acetate + reduced benzyl viologen
-
?
1.2.7.5 benzaldehyde + H2O + oxidized benzyl viologen at 149% of the activity with crotonaldehyde Pyrobaculum aerophilum benzoate + reduced benzyl viologen
-
?
1.2.7.5 cinnamaldehyde + H2O + oxidized benzyl viologen at 139% of the activity with crotonaldehyde Pyrobaculum aerophilum cinnamate + reduced benzyl viologen
-
?
1.2.7.5 crotonaldehyde + H2O + oxidized benzyl viologen
-
Pyrobaculum aerophilum crotonate + reduced benzyl viologen
-
?
1.2.7.5 crotonaldehyde + H2O + oxidized ferredoxin utilizes a 7Fe ferredoxin as the putative physiological redox partner, instead of a 4Fe ferredoxin as in Pyrococcus furiosus, 17% of the activity with benzyl viologen Pyrobaculum aerophilum crotonate + reduced ferredoxin
-
?
1.2.7.5 formaldehyde + H2O + oxidized benzyl viologen
-
Pyrobaculum aerophilum formate + reduced benzyl viologen
-
?

Subunits

EC Number Subunits Comment Organism
1.2.7.5 dimer 2 * 68000, SDS-PAGE Pyrobaculum aerophilum

Synonyms

EC Number Synonyms Comment Organism
1.2.7.5 AOR
-
Pyrobaculum aerophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.5 100
-
-
Pyrobaculum aerophilum