BRENDA - Enzyme Database

Cytosolic NADP+-dependent isocitrate dehydrogenase plays a key role in lipid metabolism

Koh, H.J.; Lee, S.M.; Son, B.G.; Lee, S.H.; Ryoo, Z.Y.; Chang, K.T.; Park, J.W.; Park, D.C.; Song, B.J.; Veech, R.L.; Song, H.; Huh, T.L.; J. Biol. Chem. 279, 39968-39974 (2004)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.42
ADP
-
Mus musculus
Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.42
expression of the cDNA in a construct of nucleotides 1-1714 with rat PEPCK promotor and the SV-40 polyadenylation signal, permanant expression in 3T3-L1 cells and in transgenic mice, the latter are constructed by infection of fertilized eggs
Mus musculus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.42
additional information
construction of transgenic mice by infection of fertilized eggs, enzyme overexpressing transgenic mice show increased triglyceride and cholesterol levels and develop fatty liver, hyperlipidemia, and obesity
Mus musculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
cytosol
-
Mus musculus
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
-
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Mus musculus
cytosolic enzyme is critical in fat and cholesterol biosynthesis, enzyme content correlates with adipogenesis in wild-type adipocytes and in transgenic cells
2-oxoglutarate + CO2 + NADPH
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Mus musculus
-
26-weeks-old male mice
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
adipocyte
-
Mus musculus
-
1.1.1.42
adipose tissue
-
Mus musculus
-
1.1.1.42
epididymis
epididymal fat pad
Mus musculus
-
1.1.1.42
fat pad
epididymal fat pad, hepatic fat pad
Mus musculus
-
1.1.1.42
liver
-
Mus musculus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.42
18.6
-
wild-type epidydymal fat pad
Mus musculus
1.1.1.42
50
-
transgenic mice epidydymal fat pad
Mus musculus
1.1.1.42
77.4
-
wild-type liver
Mus musculus
1.1.1.42
108.2
-
transgenic mice liver
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
cytosolic enzyme is critical in fat and cholesterol biosynthesis, enzyme content correlates with adipogenesis in wild-type adipocytes and in transgenic cells
656247
Mus musculus
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.42
threo-Ds-isocitrate + NADP+
-
656247
Mus musculus
2-oxoglutarate + CO2 + NADPH
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.42
25
-
assay at
Mus musculus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.2
-
assay at
Mus musculus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
essential
Mus musculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.42
ADP
-
Mus musculus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
expression of the cDNA in a construct of nucleotides 1-1714 with rat PEPCK promotor and the SV-40 polyadenylation signal, permanant expression in 3T3-L1 cells and in transgenic mice, the latter are constructed by infection of fertilized eggs
Mus musculus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
essential
Mus musculus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.42
additional information
construction of transgenic mice by infection of fertilized eggs, enzyme overexpressing transgenic mice show increased triglyceride and cholesterol levels and develop fatty liver, hyperlipidemia, and obesity
Mus musculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
cytosol
-
Mus musculus
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
-
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Mus musculus
cytosolic enzyme is critical in fat and cholesterol biosynthesis, enzyme content correlates with adipogenesis in wild-type adipocytes and in transgenic cells
2-oxoglutarate + CO2 + NADPH
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
adipocyte
-
Mus musculus
-
1.1.1.42
adipose tissue
-
Mus musculus
-
1.1.1.42
epididymis
epididymal fat pad
Mus musculus
-
1.1.1.42
fat pad
epididymal fat pad, hepatic fat pad
Mus musculus
-
1.1.1.42
liver
-
Mus musculus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.42
18.6
-
wild-type epidydymal fat pad
Mus musculus
1.1.1.42
50
-
transgenic mice epidydymal fat pad
Mus musculus
1.1.1.42
77.4
-
wild-type liver
Mus musculus
1.1.1.42
108.2
-
transgenic mice liver
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
cytosolic enzyme is critical in fat and cholesterol biosynthesis, enzyme content correlates with adipogenesis in wild-type adipocytes and in transgenic cells
656247
Mus musculus
2-oxoglutarate + CO2 + NADPH
-
-
-
?
1.1.1.42
threo-Ds-isocitrate + NADP+
-
656247
Mus musculus
2-oxoglutarate + CO2 + NADPH
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.42
25
-
assay at
Mus musculus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.2
-
assay at
Mus musculus