EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.3.38 | mutant enzymes I238T, F454Y, E520G and T505S are overexpressed in Escherichia coli | Penicillium simplicissimum |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.3.38 | hanging-drop vapor diffusion method. Crystal structures of mutant enzymes I238T, F454Y, E502G and T505S are highly similar to that of wild-type enzyme | Penicillium simplicissimum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.3.38 | E502G | the octamer/dimer ratio is 1:10. The catalytic efficiency of the mutant is significantly increased for ortho-substituted 4-methylphenols | Penicillium simplicissimum |
1.1.3.38 | F454Y | as for wild-type enzyme the octamer/dimer ratio of the mutant enzyme is 1.5:1. The catalytic efficiency of the mutant is significantly increased for ortho-substituted 4-methylphenols | Penicillium simplicissimum |
1.1.3.38 | I238T | the octamer/dimer ratio is 4:1. The catalytic efficiency of the mutant is significantly increased for ortho-substituted 4-methylphenols | Penicillium simplicissimum |
1.1.3.38 | T505S | as for wild-type enzyme the octamer/dimer ratio of the mutant enzyme is 1.5:1 | Penicillium simplicissimum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.38 | 0.0002 | - |
eugenol | pH 10, 25°C, mutant enzyme I238T | Penicillium simplicissimum | |
1.1.3.38 | 0.0008 | - |
eugenol | pH 10, 25°C, mutant enzyme F454Y | Penicillium simplicissimum | |
1.1.3.38 | 0.0008 | - |
eugenol | pH 7.5, 25°C, mutant enzyme T505S | Penicillium simplicissimum | |
1.1.3.38 | 0.001 | - |
2-methyl-p-cresol | pH 10, 25°C, mutant enzyme E502G | Penicillium simplicissimum | |
1.1.3.38 | 0.001 | - |
creosol | pH 10, 25°C, mutant enzyme I238T | Penicillium simplicissimum | |
1.1.3.38 | 0.001 | - |
creosol | pH 10, 25°C, mutant enzyme T505S | Penicillium simplicissimum | |
1.1.3.38 | 0.002 | - |
eugenol | pH 7.5, 25°C, wild-type enzyme | Penicillium simplicissimum | |
1.1.3.38 | 0.002 | - |
creosol | pH 10, 25°C, mutant enzyme F454Y | Penicillium simplicissimum | |
1.1.3.38 | 0.002 | - |
eugenol | pH 10, 25°C, mutant enzyme T505S | Penicillium simplicissimum | |
1.1.3.38 | 0.002 | - |
creosol | pH 10, 25°C, wild-type enzyme | Penicillium simplicissimum | |
1.1.3.38 | 0.003 | - |
eugenol | pH 7.5, 25°C, mutant enzyme F454Y | Penicillium simplicissimum | |
1.1.3.38 | 0.004 | - |
4-(Methoxymethyl)phenol | pH 10, 25°C, mutant enzyme E502G | Penicillium simplicissimum | |
1.1.3.38 | 0.005 | - |
eugenol | pH 7.5, 25°C, mutant enzyme E502G | Penicillium simplicissimum | |
1.1.3.38 | 0.006 | - |
2-methyl-p-cresol | pH 7.5, 25°C, mutant enzyme F454Y | Penicillium simplicissimum | |
1.1.3.38 | 0.006 | - |
Vanillyl alcohol | pH 7.5, 25°C, mutant enzyme T505S | Penicillium simplicissimum | |
1.1.3.38 | 0.007 | - |
2-methyl-p-cresol | pH 7.5, 25°C, mutant enzyme E502G | Penicillium simplicissimum | |
1.1.3.38 | 0.007 | - |
Vanillyl alcohol | pH 7.5, 25°C, mutant enzyme F454Y | Penicillium simplicissimum | |
1.1.3.38 | 0.007 | - |
Vanillyl alcohol | pH 7.5, 25°C, mutant enzyme I238T | Penicillium simplicissimum | |
1.1.3.38 | 0.007 | - |
2-methyl-p-cresol | pH 7.5, 25°C, mutant enzyme T505S | Penicillium simplicissimum | |
1.1.3.38 | 0.008 | - |
eugenol | pH 7.5, 25°C, mutant enzyme I238T | Penicillium simplicissimum | |
1.1.3.38 | 0.01 | - |
Vanillyl alcohol | pH 10, 25°C, mutant enzyme E502G | Penicillium simplicissimum | |
1.1.3.38 | 0.01 | - |
Vanillyl alcohol | pH 10, 25°C, mutant enzyme T505S | Penicillium simplicissimum | |
1.1.3.38 | 0.013 | - |
creosol | pH 7.5, 25°C, mutant enzyme F454Y | Penicillium simplicissimum | |
1.1.3.38 | 0.014 | - |
4-(Methoxymethyl)phenol | pH 10, 25°C, mutant enzyme T505S | Penicillium simplicissimum | |
1.1.3.38 | 0.015 | - |
Vanillyl alcohol | pH 10, 25°C, mutant enzyme F454Y | Penicillium simplicissimum | |
1.1.3.38 | 0.017 | - |
2-methyl-p-cresol | pH 7.5, 25°C, mutant enzyme I238T | Penicillium simplicissimum | |
1.1.3.38 | 0.019 | - |
eugenol | pH 10, 25°C, wild-type enzyme | Penicillium simplicissimum | |
1.1.3.38 | 0.02 | - |
creosol | pH 7.5, 25°C, wild-type enzyme | Penicillium simplicissimum | |
1.1.3.38 | 0.02 | - |
4-(Methoxymethyl)phenol | pH 10, 25°C, mutant enzyme I238T | Penicillium simplicissimum | |
1.1.3.38 | 0.021 | - |
2-methyl-p-cresol | pH 7.5, 25°C, wild-type enzyme | Penicillium simplicissimum | |
1.1.3.38 | 0.022 | - |
Vanillyl alcohol | pH 10, 25°C, mutant enzyme I238T | Penicillium simplicissimum | |
1.1.3.38 | 0.023 | - |
4-(Methoxymethyl)phenol | pH 10, 25°C, mutant enzyme F454Y | Penicillium simplicissimum | |
1.1.3.38 | 0.027 | - |
creosol | pH 7.5, 25°C, mutant enzyme E502G | Penicillium simplicissimum | |
1.1.3.38 | 0.029 | - |
2-amino-p-cresol | pH 10, 25°C, mutant enzyme T505S | Penicillium simplicissimum | |
1.1.3.38 | 0.031 | - |
p-Cresol | pH 7.5, 25°C, wild-type enzyme | Penicillium simplicissimum | |
1.1.3.38 | 0.031 | - |
creosol | pH 7.5, 25°C, mutant enzyme T505S | Penicillium simplicissimum | |
1.1.3.38 | 0.033 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°C, mutant enzyme T505S | Penicillium simplicissimum | |
1.1.3.38 | 0.041 | - |
creosol | pH 7.5, 25°C, mutant enzyme I238T | Penicillium simplicissimum | |
1.1.3.38 | 0.043 | - |
2-amino-p-cresol | pH 10, 25°C, mutant enzyme E502G | Penicillium simplicissimum | |
1.1.3.38 | 0.05 | - |
2-amino-p-cresol | pH 10, 25°C, mutant enzyme I238T | Penicillium simplicissimum | |
1.1.3.38 | 0.051 | - |
Vanillyl alcohol | pH 7.5, 25°C, mutant enzyme E502G | Penicillium simplicissimum | |
1.1.3.38 | 0.051 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°C, mutant enzyme I238T | Penicillium simplicissimum | |
1.1.3.38 | 0.054 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°C, mutant enzyme F454Y | Penicillium simplicissimum | |
1.1.3.38 | 0.058 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°C, wild-type enzyme | Penicillium simplicissimum | |
1.1.3.38 | 0.064 | - |
2-amino-p-cresol | pH 10, 25°C, mutant enzyme F454Y | Penicillium simplicissimum | |
1.1.3.38 | 0.064 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°C, mutant enzyme E502G | Penicillium simplicissimum | |
1.1.3.38 | 0.075 | - |
Vanillyl alcohol | pH 7.5, 25°C, wild-type enzyme | Penicillium simplicissimum | |
1.1.3.38 | 0.11 | - |
4-(Methoxymethyl)phenol | pH 10, 25°C, wild-type enzyme | Penicillium simplicissimum | |
1.1.3.38 | 0.118 | - |
2-amino-p-cresol | pH 7.5, 25°C, mutant enzyme I238T | Penicillium simplicissimum | |
1.1.3.38 | 0.123 | - |
2-amino-p-cresol | pH 7.5, 25°C, wild-type enzyme | Penicillium simplicissimum | |
1.1.3.38 | 0.167 | - |
2-amino-p-cresol | pH 7.5, 25°C, mutant enzyme F454Y | Penicillium simplicissimum | |
1.1.3.38 | 0.189 | - |
Vanillyl alcohol | pH 10, 25°C, wild-type enzyme | Penicillium simplicissimum | |
1.1.3.38 | 0.2 | - |
2-amino-p-cresol | pH 7.5, 25°C, mutant enzyme E502G | Penicillium simplicissimum | |
1.1.3.38 | 0.254 | - |
2-amino-p-cresol | pH 7.5, 25°C, mutant enzyme T505S | Penicillium simplicissimum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.38 | Penicillium simplicissimum | P56216 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.3.38 | - |
Penicillium simplicissimum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.38 | 2-amino-p-cresol + O2 | - |
Penicillium simplicissimum | ? | - |
? | |
1.1.3.38 | 2-methyl-p-cresol + O2 | - |
Penicillium simplicissimum | ? | - |
? | |
1.1.3.38 | 4-(methoxymethyl)phenol + O2 | - |
Penicillium simplicissimum | ? | - |
? | |
1.1.3.38 | creosol + O2 | - |
Penicillium simplicissimum | ? | - |
? | |
1.1.3.38 | eugenol + O2 | - |
Penicillium simplicissimum | ? | - |
? | |
1.1.3.38 | p-cresol + O2 | - |
Penicillium simplicissimum | ? | - |
? | |
1.1.3.38 | vanillyl alcohol + O2 | - |
Penicillium simplicissimum | vanillin + H2O2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.3.38 | More | octamer/dimer ratio is 1.5:1 for wild-type enzyme and mutant enzymes F454Y and T505S. For I238T the octamer/dimer ratio is 4:1. For E502G the octamer/dimer ratio is 1:10 | Penicillium simplicissimum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.38 | 0.001 | - |
2-amino-p-cresol | pH 10, 25°C, wild-type enzyme | Penicillium simplicissimum | |
1.1.3.38 | 0.002 | - |
2-methyl-p-cresol | pH 10, 25°C, wild-type enzyme | Penicillium simplicissimum | |
1.1.3.38 | 0.003 | - |
creosol | pH 8, 25°C, wild-type enzyme | Penicillium simplicissimum | |
1.1.3.38 | 0.005 | - |
creosol | pH 10, 25°C, wild-type enzyme | Penicillium simplicissimum | |
1.1.3.38 | 0.01 | - |
2-methyl-p-cresol | pH 10, 25°C, mutant enzyme F454Y | Penicillium simplicissimum | |
1.1.3.38 | 0.01 | - |
creosol | pH 8, 25°C, mutant enzyme T505S | Penicillium simplicissimum | |
1.1.3.38 | 0.013 | - |
creosol | pH 10, 25°C, mutant enzyme F454Y | Penicillium simplicissimum | |
1.1.3.38 | 0.013 | - |
creosol | pH 8, 25°C, wild-type enzyme | Penicillium simplicissimum | |
1.1.3.38 | 0.015 | - |
creosol | pH 10, 25°C, mutant enzyme A429V | Penicillium simplicissimum | |
1.1.3.38 | 0.015 | - |
creosol | pH 8, 25°C, mutant enzyme I237T/M437L | Penicillium simplicissimum | |
1.1.3.38 | 0.016 | - |
creosol | pH 8, 25°C, mutant enzyme E502G | Penicillium simplicissimum | |
1.1.3.38 | 0.017 | - |
2-amino-p-cresol | pH 7.5, 25°C, wild-type enzyme | Penicillium simplicissimum | |
1.1.3.38 | 0.017 | - |
creosol | pH 10, 25°C, mutant enzyme T505S | Penicillium simplicissimum | |
1.1.3.38 | 0.017 | - |
creosol | pH 8, 25°C, mutant enzyme F454Y | Penicillium simplicissimum | |
1.1.3.38 | 0.018 | - |
creosol | pH 8, 25°C, mutant enzyme A429V | Penicillium simplicissimum | |
1.1.3.38 | 0.019 | - |
creosol | pH 10, 25°C, mutant enzyme F93Y/P189S/I238T | Penicillium simplicissimum | |
1.1.3.38 | 0.019 | - |
2-methyl-p-cresol | pH 7.5, 25°C, mutant enzyme E502G | Penicillium simplicissimum | |
1.1.3.38 | 0.02 | - |
creosol | pH 7.5, 25°C, wild-type enzyme | Penicillium simplicissimum | |
1.1.3.38 | 0.02 | - |
creosol | pH 10, 25°C, mutant enzyme E502G | Penicillium simplicissimum | |
1.1.3.38 | 0.02 | - |
creosol | pH 10, 25°C, mutant enzyme I238T | Penicillium simplicissimum | |
1.1.3.38 | 0.02 | - |
2-methyl-p-cresol | pH 10, 25°C, mutant enzyme I238T | Penicillium simplicissimum | |
1.1.3.38 | 0.021 | - |
creosol | pH 10, 25°C, mutant enzyme I237T/M437L | Penicillium simplicissimum | |
1.1.3.38 | 0.022 | - |
creosol | pH 8, 25°C, mutant enzyme I238T | Penicillium simplicissimum | |
1.1.3.38 | 0.023 | - |
2-methyl-p-cresol | pH 10, 25°C, mutant enzyme T505S | Penicillium simplicissimum | |
1.1.3.38 | 0.025 | - |
2-methyl-p-cresol | pH 7.5, 25°C, mutant enzyme T505S | Penicillium simplicissimum | |
1.1.3.38 | 0.028 | - |
2-methyl-p-cresol | pH 10, 25°C, mutant enzyme E502G | Penicillium simplicissimum | |
1.1.3.38 | 0.031 | - |
2-methyl-p-cresol | pH 7.5, 25°C, wild-type enzyme | Penicillium simplicissimum | |
1.1.3.38 | 0.032 | - |
2-methyl-p-cresol | pH 7.5, 25°C, mutant enzyme F454Y | Penicillium simplicissimum | |
1.1.3.38 | 0.046 | - |
2-amino-p-cresol | pH 10, 25°C, mutant enzyme T505S | Penicillium simplicissimum | |
1.1.3.38 | 0.047 | - |
2-amino-p-cresol | pH 10, 25°C, mutant enzyme F454Y | Penicillium simplicissimum | |
1.1.3.38 | 0.063 | - |
2-methyl-p-cresol | pH 7.5, 25°C, mutant enzyme I238T | Penicillium simplicissimum | |
1.1.3.38 | 0.07 | - |
creosol | pH 10, 25°C, mutant enzyme I238T | Penicillium simplicissimum | |
1.1.3.38 | 0.076 | - |
2-amino-p-cresol | pH 10, 25°C, mutant enzyme E502G | Penicillium simplicissimum | |
1.1.3.38 | 0.079 | - |
2-amino-p-cresol | pH 7.5, 25°C, mutant enzyme I238T | Penicillium simplicissimum | |
1.1.3.38 | 0.082 | - |
2-amino-p-cresol | pH 7.5, 25°C, mutant enzyme F454Y | Penicillium simplicissimum | |
1.1.3.38 | 0.086 | - |
2-amino-p-cresol | pH 10, 25°C, mutant enzyme I238T | Penicillium simplicissimum | |
1.1.3.38 | 0.087 | - |
2-amino-p-cresol | pH 7.5, 25°C, mutant enzyme T505S | Penicillium simplicissimum | |
1.1.3.38 | 0.09 | - |
creosol | pH 10, 25°C, mutant enzyme E502G | Penicillium simplicissimum | |
1.1.3.38 | 0.099 | - |
2-amino-p-cresol | pH 7.5, 25°C, mutant enzyme E502G | Penicillium simplicissimum | |
1.1.3.38 | 0.1 | - |
eugenol | pH 10, 25°C, mutant enzyme E502G | Penicillium simplicissimum | |
1.1.3.38 | 0.1 | - |
creosol | pH 10, 25°C, mutant enzyme F454Y | Penicillium simplicissimum | |
1.1.3.38 | 0.1 | - |
creosol | pH 10, 25°C, mutant enzyme T505S | Penicillium simplicissimum | |
1.1.3.38 | 0.1 | - |
creosol | pH 7.5, 25°C, mutant enzyme E502G | Penicillium simplicissimum | |
1.1.3.38 | 0.12 | - |
creosol | pH 7.5, 25°C, mutant enzyme T505S | Penicillium simplicissimum | |
1.1.3.38 | 0.14 | - |
creosol | pH 7.5, 25°C, mutant enzyme F454Y | Penicillium simplicissimum | |
1.1.3.38 | 0.17 | - |
eugenol | pH 7.5, 25°C, mutant enzyme E502G | Penicillium simplicissimum | |
1.1.3.38 | 0.17 | - |
creosol | pH 7.5, 25°C, mutant enzyme I238T | Penicillium simplicissimum | |
1.1.3.38 | 0.21 | - |
Vanillyl alcohol | pH 7.5, 25°C, mutant enzyme T505S | Penicillium simplicissimum | |
1.1.3.38 | 0.22 | - |
Vanillyl alcohol | pH 7.5, 25°C, mutant enzyme E502G | Penicillium simplicissimum | |
1.1.3.38 | 0.23 | - |
eugenol | pH 10, 25°C, mutant enzyme F454Y | Penicillium simplicissimum | |
1.1.3.38 | 0.25 | - |
Vanillyl alcohol | pH 7.5, 25°C, mutant enzyme F454Y | Penicillium simplicissimum | |
1.1.3.38 | 0.3 | - |
Vanillyl alcohol | pH 10, 25°C, mutant enzyme E502G | Penicillium simplicissimum | |
1.1.3.38 | 0.39 | - |
Vanillyl alcohol | pH 7.5, 25°C, mutant enzyme I238T | Penicillium simplicissimum | |
1.1.3.38 | 0.5 | - |
eugenol | pH 7.5, 25°C, mutant enzyme F454Y | Penicillium simplicissimum | |
1.1.3.38 | 0.55 | - |
eugenol | pH 10, 25°C, mutant enzyme T505S | Penicillium simplicissimum | |
1.1.3.38 | 0.6 | - |
eugenol | pH 10, 25°C, mutant enzyme I238T | Penicillium simplicissimum | |
1.1.3.38 | 0.65 | - |
eugenol | pH 7.5, 25°C, mutant enzyme T505S | Penicillium simplicissimum | |
1.1.3.38 | 0.67 | - |
Vanillyl alcohol | pH 10, 25°C, mutant enzyme T505S | Penicillium simplicissimum | |
1.1.3.38 | 0.71 | - |
Vanillyl alcohol | pH 10, 25°C, mutant enzyme F454Y | Penicillium simplicissimum | |
1.1.3.38 | 0.8 | - |
eugenol | pH 7.5, 25°C, mutant enzyme I238T | Penicillium simplicissimum | |
1.1.3.38 | 1.1 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°C, mutant enzyme E502G | Penicillium simplicissimum | |
1.1.3.38 | 1.2 | - |
4-(Methoxymethyl)phenol | pH 10, 25°C, mutant enzyme E502G | Penicillium simplicissimum | |
1.1.3.38 | 1.4 | - |
Vanillyl alcohol | pH 10, 25°C, mutant enzyme I238T | Penicillium simplicissimum | |
1.1.3.38 | 1.6 | - |
Vanillyl alcohol | pH 7.5, 25°C, wild-type enzyme | Penicillium simplicissimum | |
1.1.3.38 | 2.1 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°C, mutant enzyme F454Y | Penicillium simplicissimum | |
1.1.3.38 | 2.1 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°C, mutant enzyme I238T | Penicillium simplicissimum | |
1.1.3.38 | 2.3 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°C, mutant enzyme T505S | Penicillium simplicissimum | |
1.1.3.38 | 2.4 | - |
Vanillyl alcohol | pH 10, 25°C, wild-type enzyme | Penicillium simplicissimum | |
1.1.3.38 | 3 | - |
4-(Methoxymethyl)phenol | pH 10, 25°C, mutant enzyme I238T | Penicillium simplicissimum | |
1.1.3.38 | 3.1 | - |
4-(Methoxymethyl)phenol | pH 7.5, 25°C, wild-type enzyme | Penicillium simplicissimum | |
1.1.3.38 | 3.4 | - |
4-(Methoxymethyl)phenol | pH 10, 25°C, mutant enzyme F454Y | Penicillium simplicissimum | |
1.1.3.38 | 3.6 | - |
4-(Methoxymethyl)phenol | pH 10, 25°C, mutant enzyme T505S | Penicillium simplicissimum | |
1.1.3.38 | 3.9 | - |
4-(Methoxymethyl)phenol | pH 10, 25°C, wild-type enzyme | Penicillium simplicissimum | |
1.1.3.38 | 14 | - |
eugenol | pH 7.5, 25°C, wild-type enzyme | Penicillium simplicissimum | |
1.1.3.38 | 39 | - |
eugenol | pH 10, 25°C, wild-type enzyme | Penicillium simplicissimum |