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Literature summary extracted from
- The Fourth Transmembrane Segment of the Na,K-ATPase a Subunit: A systematic mutagenesis study (2004), J. Biol. Chem., 279, 29542-29550.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 7.2.2.13 | expression in Xenopus laevis oocyte | Rhinella marina |
| 7.2.2.13 | in pSD5 vector, expression of proteins in Xenopus laevis oocytes | Rhinella marina |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 7.2.2.13 | A330C | difference between K+-induced and ouabain-sensitive I/V curve especially in the negative voltage range, negative slope of the I/V curve of the ouabain-sensitive current in the low and positive membrane potential range | Rhinella marina |
| 7.2.2.13 | A330C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, lower than control | Rhinella marina |
| 7.2.2.13 | A338C | difference between K+-induced and ouabain-sensitive I/V curve especially in the negative voltage range | Rhinella marina |
| 7.2.2.13 | A338C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, lower than control | Rhinella marina |
| 7.2.2.13 | C111S | little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range | Rhinella marina |
| 7.2.2.13 | C111S | used as control | Rhinella marina |
| 7.2.2.13 | E334C | negative slope of the I/V curve of the ouabain-sensitive current in the low and positive membrane potential range, K+-activated current with an amplitude half of the oubain-sensitive current in the high negative membrane potential range | Rhinella marina |
| 7.2.2.13 | E334C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, lower than control | Rhinella marina |
| 7.2.2.13 | F323C | small ouabain-sensitive current over the whole membrane potential range, significant K+-induced inward curve mostly at negative membrane potential range | Rhinella marina |
| 7.2.2.13 | F323C | K+ induced current, I_K in nA, lower than control(negativ) - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control | Rhinella marina |
| 7.2.2.13 | G326C | inhibition by 2-aminoethyl-methanethiosulfonate | Rhinella marina |
| 7.2.2.13 | G326C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, lower than control | Rhinella marina |
| 7.2.2.13 | G335C | identical and strongly voltage-dependent I/V relationship of the K+-induced and ouabain sensitive currents | Rhinella marina |
| 7.2.2.13 | G335C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control | Rhinella marina |
| 7.2.2.13 | I322C | little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range | Rhinella marina |
| 7.2.2.13 | I322C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control | Rhinella marina |
| 7.2.2.13 | I325C | little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range | Rhinella marina |
| 7.2.2.13 | I325C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control | Rhinella marina |
| 7.2.2.13 | I327C | difference between K+-induced and ouabain-sensitive I/V curve especially in the negative voltage range | Rhinella marina |
| 7.2.2.13 | I327C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control | Rhinella marina |
| 7.2.2.13 | I328C | negative slope of the I/V curve of the ouabain-sensitive current in the low and positive membrane potential range | Rhinella marina |
| 7.2.2.13 | I328C | K+ induced current, I_K in nA, higher than control - ouabain-sensitive current, I_ouab in nA, higher than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control | Rhinella marina |
| 7.2.2.13 | L324C | little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range | Rhinella marina |
| 7.2.2.13 | L324C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, similar to control | Rhinella marina |
| 7.2.2.13 | L336C | little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range | Rhinella marina |
| 7.2.2.13 | L336C | K+ induced current, I_K in nA, higher than control - ouabain-sensitive current, I_ouab in nA, higher than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control | Rhinella marina |
| 7.2.2.13 | L337C | K+ induced current, I_K in nA, lower (negative) than control - ouabain-sensitive current, I_ouab in nA, lower (negative) than control - membrane conductance induced by palytoxin, G_PTX in mikroS, lower than control | Rhinella marina |
| 7.2.2.13 | L344C | little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range | Rhinella marina |
| 7.2.2.13 | L344C | K+ induced current, I_K in nA, higher than control - ouabain-sensitive current, I_ouab in nA, higher than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control | Rhinella marina |
| 7.2.2.13 | N331C | difference between K+-induced and ouabain-sensitive I/V curve especially in the negative voltage range | Rhinella marina |
| 7.2.2.13 | N331C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, similar to control | Rhinella marina |
| 7.2.2.13 | P333C | inhibition by 2-aminoethyl-methanethiosulfonate | Rhinella marina |
| 7.2.2.13 | P333C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control | Rhinella marina |
| 7.2.2.13 | T339C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, lower control | Rhinella marina |
| 7.2.2.13 | T341C | K+ induced current, I_K in nA, higher than control - ouabain-sensitive current, I_ouab in nA, similar to control - membrane conductance induced by palytoxin, G_PTX in mikroS, lower than control | Rhinella marina |
| 7.2.2.13 | V321C | little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range | Rhinella marina |
| 7.2.2.13 | V321C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, similar to control | Rhinella marina |
| 7.2.2.13 | V329C | little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range | Rhinella marina |
| 7.2.2.13 | V329C | K+ induced current, I_K in nA, lower than control - ouabain-sensitive current, I_ouab in nA, lower than control - membrane conductance induced by palytoxin, G_PTX in mikroS, similar to control | Rhinella marina |
| 7.2.2.13 | V332C | little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range | Rhinella marina |
| 7.2.2.13 | V332C | K+ induced current, I_K in nA, similar to control - ouabain-sensitive current, I_ouab in nA, similar to control - membrane conductance induced by palytoxin, G_PTX in mikroS, similar to control | Rhinella marina |
| 7.2.2.13 | V340C | little voltage dependence in the low membrane voltage range and a slight voltage dependence in the high negative membrane voltage range | Rhinella marina |
| 7.2.2.13 | V340C | K+ induced current, I_K in nA, higher than control - ouabain-sensitive current, I_ouab in nA, higher than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control | Rhinella marina |
| 7.2.2.13 | V342C | K+ induced current, I_K in nA, higher than control - ouabain-sensitive current, I_ouab in nA, higher than control - membrane conductance induced by palytoxin, G_PTX in mikroS, higher than control | Rhinella marina |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.2.2.13 | 2-aminoethyl-methanethiosulfonate | inhibition of mutants F323C, P333C, E334C, G335C, I322C, G326C and A330C | Rhinella marina |  |
| 7.2.2.13 | Ouabain | g-strophanthin | Rhinella marina | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.2.2.13 | membrane | - |
Rhinella marina | 16020 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.2.2.13 | ADP + phosphate + Na+/out | Rhinella marina | - |
ATP + H2O + Na+/in | - |
? | |
| 7.2.2.13 | ATP + H2O + K+/in | Rhinella marina | - |
ADP + phosphate + K+/out | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.2.2.13 | Rhinella marina | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.2.2.13 | ADP + phosphate + Na+/out | - |
Rhinella marina | ATP + H2O + Na+/in | - |
? | |
| 7.2.2.13 | ATP + H2O + K+/in | - |
Rhinella marina | ADP + phosphate + K+/out | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 7.2.2.13 | dimer | alpha subunit, main subunit - beta subunit, glycoprotein | Rhinella marina |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.2.2.13 | Na,K-ATPase | - |
Rhinella marina |
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