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Literature summary extracted from

  • Tani, M.; Okino, N.; Sueyoshi, N.; Ito, M.
    Conserved amino acid residues in the COOH-terminal tail are indispensable for the correct folding and localization and enzyme activity of neutral ceramidase (2004), J. Biol. Chem., 279, 29351-29358.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.23 expression in Escherichia coli BL21(DE3) Pseudomonas aeruginosa
3.5.1.23 wild-type and C-terminal truncated CDase, expression in HEK-293 cells Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.23 E757R mutation has no effect on activity, subcellular localization of mutant CDase expressed in HEK-293 cells Rattus norvegicus
3.5.1.23 F756D inactive mutant, subcellular localization of mutant CDase expressed in HEK-293 cells Rattus norvegicus
3.5.1.23 F756I mutation has little effect on activity, subcellular localization of mutant CDase expressed in HEK-293 cells Rattus norvegicus
3.5.1.23 F756R inactive mutant, subcellular localization of mutant CDase expressed in HEK-293 cells Rattus norvegicus
3.5.1.23 I758D inactive mutant, subcellular localization of mutant CDase expressed in HEK-293 cells Rattus norvegicus
3.5.1.23 I758F mutation decreases activity to 20% of wild-type CDase activity, subcellular localization of mutant CDase expressed in HEK-293 cells Rattus norvegicus
3.5.1.23 I758R inactive mutant, subcellular localization of mutant CDase expressed in HEK-293 cells Rattus norvegicus
3.5.1.23 I758V same activity as wild-type CDase, subcellular localization of mutant CDase expressed in HEK-293 cells Rattus norvegicus
3.5.1.23 additional information truncation of four, but not three, amino acid residues at the C-terminus results in a complete loss of activity as well as surface expression in HEK-293 cells, the truncated enzymes are retained in the endoplasmic reticulum and rapidly degraded without transportation to the Golgi apparatus also leading to an immature/incorrect glycosylation of the protein Rattus norvegicus
3.5.1.23 additional information truncation of six C-terminal amino acids, but not of five, results in complete loss of CDase activity Pseudomonas aeruginosa
3.5.1.23 V665D inactive mutant Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.23 endoplasmic reticulum 113 kDa developing form, enzyme which is truncated at the C-terminus by four amino acids is retained in the endoplasmic reticulum and rapidly degraded without transportation to the Golgi apparatus Rattus norvegicus 5783
-
3.5.1.23 Golgi apparatus 133 kDa mature form Rattus norvegicus 5794
-
3.5.1.23 additional information subcellular localization of wild-type and several C-terminal truncated CDases Rattus norvegicus
-
-
3.5.1.23 plasma membrane wild-type CDase is located at plasma membranes Rattus norvegicus 5886
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.23 additional information Rattus norvegicus neutral ceramidase is involved in the regulation of ceramide-mediated signaling ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.23 Pseudomonas aeruginosa
-
-
-
3.5.1.23 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.23 glycoprotein
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.23 4-nitrobenzo-2-oxa-1,3-diazoyl-N-dodecanoylsphingosine + H2O
-
 Rattus norvegicus 4-nitrobenzo-2-oxa-1,3-diazoyl-dodecanoic acid + sphingosine
-
 r
3.5.1.23 additional information neutral ceramidase is involved in the regulation of ceramide-mediated signaling Rattus norvegicus ?
-
 ?
3.5.1.23 additional information the C-terminal residues Ile-758 and Phe-756 are essential for enzyme function, the C-terminal tail is indispensable for the correct folding, localization and enzyme activity Rattus norvegicus ?
-
 ?
3.5.1.23 additional information the C-terminal tail is essential for enzyme activity and correct folding Pseudomonas aeruginosa ?
-
 ?
3.5.1.23 N-acylsphingosine + H2O CDase cleaves the N-acyl linkage of ceramide to produce sphingosine and free fatty acid Rattus norvegicus a carboxylate + sphingosine
-
 ?
3.5.1.23 N-acylsphingosine + H2O CDase cleaves the N-acyl linkage of ceramide to produce sphingosine and free fatty acid Pseudomonas aeruginosa a carboxylate + sphingosine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.23 More the C-terminal tail is essential for enzyme activity and correct folding Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
3.5.1.23 CDase
-
 Rattus norvegicus
3.5.1.23 CDase
-
 Pseudomonas aeruginosa
3.5.1.23 neutral ceramidase
-
 Rattus norvegicus
3.5.1.23 neutral ceramidase
-
 Pseudomonas aeruginosa