Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Schweizer, R.A.; Zurcher, M.; Balazs, Z.; Dick, B.; Odermatt, A.
    Rapid hepatic metabolism of 7-ketocholesterol by 11beta-hydroxysteroid dehydrogenase type 1: species-specific differences between the rat, human, and hamster enzyme (2004), J. Biol. Chem., 279, 18415-18424.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.146 expression in HEK-293 cells Homo sapiens
1.1.1.146 expression in HEK-293 cells Rattus norvegicus
1.1.1.146 expression in HEK-293 cells Mesocricetus auratus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.146 2'-hydroxyflavanone 0.2 mM, more than 90% inhibition of 7-oxocholesterol reduction in liver homogenates Mesocricetus auratus
1.1.1.146 2'-hydroxyflavanone 0.2 mM, more than 90% inhibition of 7-oxocholesterol reduction in liver homogenates Rattus norvegicus
1.1.1.146 carbenoxolone 0.01 mM, more than 90% inhibition of 7-oxocholesterol reduction in liver homogenates Mesocricetus auratus
1.1.1.146 carbenoxolone 0.01 mM, more than 90% inhibition of 7-oxocholesterol reduction in liver homogenates Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.146 0.0003
-
corticosterone 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Homo sapiens
1.1.1.146 0.00031
-
11-Dehydrocorticosterone 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Homo sapiens
1.1.1.146 0.00031
-
11-Dehydrocorticosterone 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Rattus norvegicus
1.1.1.146 0.00038
-
7-oxocholesterol 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Mesocricetus auratus
1.1.1.146 0.0004
-
11-Dehydrocorticosterone 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Mesocricetus auratus
1.1.1.146 0.00042
-
7-oxocholesterol 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Rattus norvegicus
1.1.1.146 0.00049
-
7-oxocholesterol 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Homo sapiens
1.1.1.146 0.00049
-
7alpha-hydroxycholesterol 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Mesocricetus auratus
1.1.1.146 0.00073
-
7beta-hydroxycholesterol 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Mesocricetus auratus
1.1.1.146 0.00074
-
7beta-hydroxycholesterol 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Homo sapiens
1.1.1.146 0.00075
-
7beta-hydroxycholesterol 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Rattus norvegicus
1.1.1.146 0.0012
-
corticosterone 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Rattus norvegicus
1.1.1.146 0.0032
-
corticosterone 37°C, pH 7.4, in HEK-293 lysates expressing 11beta-HSD1 Mesocricetus auratus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.146 7beta-hydroxycholesterol + NADP+ Homo sapiens
-
7-oxocholesterol + NADPH
-
r
1.1.1.146 7beta-hydroxycholesterol + NADP+ Rattus norvegicus
-
7-oxocholesterol + NADPH
-
r
1.1.1.146 7beta-hydroxycholesterol + NADP+ Mesocricetus auratus
-
7-oxocholesterol + NADPH
-
r
1.1.1.146 corticosterone + NADP+ Homo sapiens
-
11-dehydrocorticosterone + NADPH
-
r
1.1.1.146 corticosterone + NADP+ Rattus norvegicus
-
11-dehydrocorticosterone + NADPH
-
r
1.1.1.146 corticosterone + NADP+ Mesocricetus auratus
-
11-dehydrocorticosterone + NADPH
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.146 Homo sapiens
-
-
-
1.1.1.146 Mesocricetus auratus Q6R0J2
-
-
1.1.1.146 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.146 kidney
-
Rattus norvegicus
-
1.1.1.146 liver
-
Homo sapiens
-
1.1.1.146 liver
-
Rattus norvegicus
-
1.1.1.146 liver
-
Mesocricetus auratus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.146 2 7-oxocholesterol + 2 NADPH + 2 H+
-
Mesocricetus auratus 7beta-hydroxycholesterol + 7alpha-hydroxycholesterol + 2 NADP+ 40% and 60%, respectively, in lysates of HEK-293 cells and intact HEK-293 cells expressing 11beta-HSD1 r
1.1.1.146 7alpha-hydroxycholesterol + NADP+ in lysates of HEK-293 cells expressing 11beta-HSD1, no oxidation in intact HEK293 cells expressing 11beta-HSD1 Mesocricetus auratus 7-oxocholesterol + NADPH
-
r
1.1.1.146 7beta-hydroxycholesterol + NADP+
-
Homo sapiens 7-oxocholesterol + NADPH
-
r
1.1.1.146 7beta-hydroxycholesterol + NADP+
-
Rattus norvegicus 7-oxocholesterol + NADPH
-
r
1.1.1.146 7beta-hydroxycholesterol + NADP+
-
Mesocricetus auratus 7-oxocholesterol + NADPH
-
r
1.1.1.146 7beta-hydroxycholesterol + NADP+ in lysates of HEK-293 cells expressing 11beta-HSD1, no oxidation in intact HEK293 cells expressing 11beta-HSD1 Homo sapiens 7-oxocholesterol + NADPH
-
r
1.1.1.146 7beta-hydroxycholesterol + NADP+ in lysates of HEK-293 cells expressing 11beta-HSD1, no oxidation in intact HEK293 cells expressing 11beta-HSD1 Rattus norvegicus 7-oxocholesterol + NADPH
-
r
1.1.1.146 7beta-hydroxycholesterol + NADP+ in lysates of HEK-293 cells expressing 11beta-HSD1, no oxidation in intact HEK293 cells expressing 11beta-HSD1 Mesocricetus auratus 7-oxocholesterol + NADPH
-
r
1.1.1.146 corticosterone + NADP+
-
Homo sapiens 11-dehydrocorticosterone + NADPH
-
r
1.1.1.146 corticosterone + NADP+
-
Rattus norvegicus 11-dehydrocorticosterone + NADPH
-
r
1.1.1.146 corticosterone + NADP+
-
Mesocricetus auratus 11-dehydrocorticosterone + NADPH
-
r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.146 11beta-HSD1
-
Homo sapiens
1.1.1.146 11beta-HSD1
-
Rattus norvegicus
1.1.1.146 11beta-HSD1
-
Mesocricetus auratus
1.1.1.146 11beta-hydroxysteroid dehydrogenase type 1
-
Homo sapiens
1.1.1.146 11beta-hydroxysteroid dehydrogenase type 1
-
Rattus norvegicus
1.1.1.146 11beta-hydroxysteroid dehydrogenase type 1
-
Mesocricetus auratus