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Literature summary extracted from

  • Verho, R.; Putkonen, M.; Londesborough, J.; Penttilä, M.; Richard, P.
    A novel NADH-linked L-xylulose reductase in the L-arabinose catabolic pathway of yeast (2004), J. Biol. Chem., 279, 14746-14751.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.10 construction of a cDNA library, DNA sequence determination and analysis, subcloning in Escherichia coli DH5alpha, overexpression as His-tagged enzyme in Saccharomyces cerevisiae strain CEN.PK2 Ambrosiozyma monospora

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.10 2.7
-
D-arabinitol pH 9.0, 30°C, purified recombinant enzyme Ambrosiozyma monospora
1.1.1.10 4.7
-
D-ribulose pH 7.0, 30°C, purified recombinant enzyme Ambrosiozyma monospora
1.1.1.10 7.2
-
L-xylitol pH 9.0, 30°C, purified recombinant enzyme Ambrosiozyma monospora
1.1.1.10 9.6
-
L-xylulose pH 7.0, 30°C, purified recombinant enzyme Ambrosiozyma monospora

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.10 Mg2+
-
Ambrosiozyma monospora

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.10 L-xylulose + NADH Ambrosiozyma monospora part of the L-arabinose catabolism L-xylitol + NAD+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.10 Ambrosiozyma monospora Q70FD1 fragment; yeast, strain NRRL Y-1484
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.10 recombinant His-tagged enzyme from Saccharomyces cerevisiae by nickel affinity chromatography Ambrosiozyma monospora

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.10 D-ribulose + NADH
-
Ambrosiozyma monospora D-arabinitol + NAD+
-
r
1.1.1.10 D-xylulose + NADH + H+ about 100fold lower activity compared to L-xylulose Ambrosiozyma monospora D-arabinitol + NAD+
-
r
1.1.1.10 L-xylulose + NADH
-
Ambrosiozyma monospora L-xylitol + NAD+
-
r
1.1.1.10 L-xylulose + NADH part of the L-arabinose catabolism Ambrosiozyma monospora L-xylitol + NAD+
-
r
1.1.1.10 additional information NADH-linked enzyme form, no activity with NADPH and NADP+, no activity with L-arabinitol, adonitol or ribitol, dulcitol or galactitol, D-mannitol, and D-sorbitol, no activity with D-sorbose, L-sorbose, D-psicose, and D-fructose Ambrosiozyma monospora ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.10 30
-
assay at Ambrosiozyma monospora

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.10 7
-
forward, reduction reaction Ambrosiozyma monospora
1.1.1.10 9
-
reverse, oxidation reaction Ambrosiozyma monospora

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.10 NAD+ specific for, no activity with NADP+ Ambrosiozyma monospora
1.1.1.10 NADH specific for no activity with NADPH Ambrosiozyma monospora