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Literature summary extracted from
- Modification of acivity and specificity of haloalkane dehalogenase from Sphingomonas paucimobilis UT26, by engineering of its entrance tunnel (2003), J. Biol. Chem., 278, 52622-52628.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.8.1.5 | expression of wild-type and mutant enzymes in Escherichia coli | Sphingomonas paucimobilis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.8.1.5 | L177A | site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme | Sphingomonas paucimobilis |
| 3.8.1.5 | L177C | site-directed mutagenesis, altered substrate specificity, especially high activity with 1-iodobutane, bromocyclohexane and 1-bromobutane | Sphingomonas paucimobilis |
| 3.8.1.5 | L177D | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Sphingomonas paucimobilis |
| 3.8.1.5 | L177E | site-directed mutagenesis, mutant cannot be expressed in Escherichia coli due to incorrect protein folding | Sphingomonas paucimobilis |
| 3.8.1.5 | L177F | site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme | Sphingomonas paucimobilis |
| 3.8.1.5 | L177H | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Sphingomonas paucimobilis |
| 3.8.1.5 | L177I | site-directed mutagenesis, inactive mutant, incorrect protein folding | Sphingomonas paucimobilis |
| 3.8.1.5 | L177M | site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme | Sphingomonas paucimobilis |
| 3.8.1.5 | L177N | site-directed mutagenesis, mutant cannot be expressed in Escherichia coli due to incorrect protein folding | Sphingomonas paucimobilis |
| 3.8.1.5 | L177P | site-directed mutagenesis, inactive mutant, incorrect protein folding | Sphingomonas paucimobilis |
| 3.8.1.5 | L177Q | site-directed mutagenesis, altered substrate specificity, especially high activity with 1-iodobutane and 1-bromobutane | Sphingomonas paucimobilis |
| 3.8.1.5 | L177R | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Sphingomonas paucimobilis |
| 3.8.1.5 | L177S | site-directed mutagenesis, altered substrate specificity, especially high activity with 1-iodobutane and 1-bromobutane | Sphingomonas paucimobilis |
| 3.8.1.5 | L177T | site-directed mutagenesis, altered substrate specificity, especially high activity with 1-iodobutane and 1-bromobutane | Sphingomonas paucimobilis |
| 3.8.1.5 | L177V | site-directed mutagenesis, altered substrate specificity, especially high activity with 1-bromobutane | Sphingomonas paucimobilis |
| 3.8.1.5 | L177W | site-directed mutagenesis, altered substrate specificity, specifically highly active with 1-chlorobutane | Sphingomonas paucimobilis |
| 3.8.1.5 | L177Y | site-directed mutagenesis, altered substrate specificity, especially low activity with 1,2-dibromoethane | Sphingomonas paucimobilis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.8.1.5 | additional information | - |
additional information | steady-state kinetics of wild-type and mutant enzymes using substrate 1-chlorobutane and 1,2-dibromoethane | Sphingomonas paucimobilis | |
| 3.8.1.5 | 0.235 | - |
1-Chlorobutane | pH 8.6, 37°C, recombinant wild-type enzyme, data from 2 experimental sets | Sphingomonas paucimobilis |  |
| 3.8.1.5 | 5.65 | - |
1,2-Dibromoethane | pH 8.6, 37°C, recombinant wild-type enzyme, data from 2 experimental sets | Sphingomonas paucimobilis | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.8.1.5 | Sphingomonas paucimobilis | - |
enzyme LinB | - |
| 3.8.1.5 | Sphingomonas paucimobilis UT26 | - |
enzyme LinB | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.8.1.5 | recombinant wild-type and mutant enzymes from Escherichia coli | Sphingomonas paucimobilis |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.8.1.5 | 1-haloalkane + H2O = a primary alcohol + halide | substrate specificity is influenced by the size and shape of their entrance tunnel, surface residue Leu177 is involved and located at the tunnel opening | Sphingomonas paucimobilis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.5 | additional information | - |
data from 2 experimental sets, relative activity of diverse L177 mutants compared to the wild-type enzyme with the different substrates, overview | Sphingomonas paucimobilis |
| 3.8.1.5 | 0.16 | - |
purified recombinant wild-type enzyme, substrate chlorocyclohexane | Sphingomonas paucimobilis |
| 3.8.1.5 | 1.54 | - |
purified recombinant wild-type enzyme, substrate 1-iodobutane | Sphingomonas paucimobilis |
| 3.8.1.5 | 1.57 | - |
purified recombinant wild-type enzyme, substrate 1-chlorohexane | Sphingomonas paucimobilis |
| 3.8.1.5 | 1.61 | - |
purified recombinant wild-type enzyme, substrate bromocyclohexane | Sphingomonas paucimobilis |
| 3.8.1.5 | 1.88 | - |
purified recombinant wild-type enzyme, substrate 1-chlorobutane | Sphingomonas paucimobilis |
| 3.8.1.5 | 2.18 | - |
purified recombinant wild-type enzyme, substrate 1,3-diiodopropane | Sphingomonas paucimobilis |
| 3.8.1.5 | 3.33 | - |
purified recombinant wild-type enzyme, substrate 1-bromoobutane | Sphingomonas paucimobilis |
| 3.8.1.5 | 8.03 | - |
purified recombinant wild-type enzyme, substrate 3-chloro-2-methylpropene | Sphingomonas paucimobilis |
| 3.8.1.5 | 12.3 | - |
purified recombinant wild-type enzyme, substrate 1,2-dibromoethane | Sphingomonas paucimobilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.8.1.5 | 1,2,3-trichloropropane + H2O | - |
Sphingomonas paucimobilis | 2,3-dichloro-1-propanol + chloride | - |
? | |
| 3.8.1.5 | 1,2,3-trichloropropane + H2O | - |
Sphingomonas paucimobilis UT26 | 2,3-dichloro-1-propanol + chloride | - |
? | |
| 3.8.1.5 | 1,2-dibromoethane + H2O | - |
Sphingomonas paucimobilis | 2-bromoethanol + bromide | - |
? | |
| 3.8.1.5 | 1,2-dibromoethane + H2O | - |
Sphingomonas paucimobilis UT26 | 2-bromoethanol + bromide | - |
? | |
| 3.8.1.5 | 1,2-dichloroethane + H2O | - |
Sphingomonas paucimobilis | 2-chloroethanol + chloride | - |
? | |
| 3.8.1.5 | 1,2-dichloroethane + H2O | - |
Sphingomonas paucimobilis UT26 | 2-chloroethanol + chloride | - |
? | |
| 3.8.1.5 | 1,2-dichloropropane + H2O | - |
Sphingomonas paucimobilis | 2-chloropropan-1-ol + chloride | - |
? | |
| 3.8.1.5 | 1,2-dichloropropane + H2O | - |
Sphingomonas paucimobilis UT26 | 2-chloropropan-1-ol + chloride | - |
? | |
| 3.8.1.5 | 1,3-diiodopropane + H2O | - |
Sphingomonas paucimobilis | 3-iodopropanol + iodide | - |
? | |
| 3.8.1.5 | 1-bromobutane + H2O | - |
Sphingomonas paucimobilis | n-butanol + bromide | - |
? | |
| 3.8.1.5 | 1-chlorobutane + H2O | - |
Sphingomonas paucimobilis | n-butanol + chloride | - |
? | |
| 3.8.1.5 | 1-chlorohexane + H2O | - |
Sphingomonas paucimobilis | n-hexanol + chloride | - |
? | |
| 3.8.1.5 | 1-iodobutane + H2O | - |
Sphingomonas paucimobilis | n-butanol + iodide | - |
? | |
| 3.8.1.5 | 3-chloro-2-methylpropene + H2O | - |
Sphingomonas paucimobilis | 2-methylpropene-3-ol + chloride | - |
? | |
| 3.8.1.5 | bromocyclohexane + H2O | - |
Sphingomonas paucimobilis | cyclohexanol + bromide | - |
? | |
| 3.8.1.5 | chlorocyclohexane + H2O | - |
Sphingomonas paucimobilis | cyclohexanol + chloride | - |
? | |
| 3.8.1.5 | additional information | substrate specificity is influenced by the size and shape of their entrance tunnel, surface residue Leu177 is involved and located at the tunnel opening | Sphingomonas paucimobilis | ? | - |
? | |
| 3.8.1.5 | additional information | substrate specificity is influenced by the size and shape of their entrance tunnel, surface residue Leu177 is involved and located at the tunnel opening | Sphingomonas paucimobilis UT26 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.8.1.5 | LinB | - |
Sphingomonas paucimobilis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.5 | 37 | - |
assay at | Sphingomonas paucimobilis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.8.1.5 | additional information | - |
additional information | - |
Sphingomonas paucimobilis | |
| 3.8.1.5 | 1.055 | - |
1-Chlorobutane | pH 8.6, 37°C, recombinant wild-type enzyme, data from 2 experimental sets | Sphingomonas paucimobilis | |
| 3.8.1.5 | 26.4 | - |
1,2-Dibromoethane | pH 8.6, 37°C, recombinant wild-type enzyme, data from 2 experimental sets | Sphingomonas paucimobilis | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.5 | 8.6 | - |
assay at | Sphingomonas paucimobilis |
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