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Literature summary extracted from

  • Jang, Y.M.; Kim, D.W.; Kang, T.C.; Won, M.H.; Baek, N.I.; Moon, B.J.; Choi, S.Y.; Kwon, O.S.
    Human pyridoxal phosphatase. Molecular cloning, functional expression, and tissue distribution (2003), J. Biol. Chem., 278, 50040-50046.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.74 cDNA, from brain, expression in Escherichia coli M15/pRER4, sequencing, ORF is located on chromosome 22q12.3, genomic organization Homo sapiens
3.1.3.74 full-length cDNA, from brain, sequencing, ORF is located on chromosome 15.E1, genomic organization Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.74 0.0025
-
 pyridoxal 5'-phosphate pH 7.4, 37°C, recombinant enzyme Homo sapiens
3.1.3.74 0.0434
-
 pyridoxine 5'-phosphate pH 7.4, 37°C, recombinant enzyme Homo sapiens
3.1.3.74 0.0806
-
 pyridoxamine 5'-phosphate pH 7.4, 37°C, recombinant enzyme Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.74 Mg2+ requirement for a divalent cation, Mg2+ is most effective in catalyzing the dephosphorylation of pyridoxal 5’-phosphate Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.74 31512
-
 x * 31512, sequence calculation Mus musculus
3.1.3.74 31698
-
 2 * 31698, sequence calculation, 2 * 32000, recombinant enzyme, SDS-PAGE Homo sapiens
3.1.3.74 32000
-
 2 * 31698, sequence calculation, 2 * 32000, recombinant enzyme, SDS-PAGE Homo sapiens
3.1.3.74 60000
-
 gel filtration, recombinant enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.74 additional information Homo sapiens vitamin B6 metabolism, catabolism of pyridoxal 5’-phosphate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.74 Homo sapiens Q96GD0
-
-
3.1.3.74 Mus musculus P60487
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.74 9.6fold, recombinant enzyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.74 brain
-
 Mus musculus
-
3.1.3.74 brain highly abundant in, most highly represented in all the regions of central nerve system except the spinal cord Homo sapiens
-
3.1.3.74 liver major expression site additional to brain and testis Homo sapiens
-
3.1.3.74 additional information mRNA is differentially expressed in a tissue-specific manner, tissue distribution Homo sapiens
-
3.1.3.74 testis major expression site additional to brain and liver Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.74 1.4
-
 pH 7.4, 37°C, hydrolysis of pyridoxal 5’-phosphate, recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.74 additional information vitamin B6 metabolism, catabolism of pyridoxal 5’-phosphate Homo sapiens ?
-
 ?
3.1.3.74 additional information very low activity with with p-nitrophenyl phosphate Homo sapiens ?
-
 ?
3.1.3.74 pyridoxal 5'-phosphate + H2O
-
 Mus musculus pyridoxal + phosphate
-
 ?
3.1.3.74 pyridoxal 5'-phosphate + H2O highest specificity constant followed by pyridoxine 5’-phosphate Homo sapiens pyridoxal + phosphate
-
 ?
3.1.3.74 pyridoxamine 5'-phosphate + H2O
-
 Homo sapiens pyridoxamine + phosphate
-
 ?
3.1.3.74 pyridoxine 5'-phosphate + H2O
-
 Mus musculus pyridoxine + phosphate
-
 ?
3.1.3.74 pyridoxine 5'-phosphate + H2O
-
 Homo sapiens pyridoxine + phosphate
-
 ?
3.1.3.74 pyridoxine 5'-phosphate + H2O second highest specificity constant after pyridoxal 5’-phosphate Homo sapiens pyridoxine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.74 ? x * 31512, sequence calculation Mus musculus
3.1.3.74 dimer 2 * 31698, sequence calculation, 2 * 32000, recombinant enzyme, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.74 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.74 0.45
-
 pyridoxamine 5'-phosphate pH 7.4, 37°C, recombinant enzyme Homo sapiens
3.1.3.74 1.25
-
 pyridoxine 5'-phosphate pH 7.4, 37°C, recombinant enzyme Homo sapiens
3.1.3.74 1.52
-
 pyridoxal 5'-phosphate pH 7.4, 37°C, recombinant enzyme Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.74 7.4
-
 assay at Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.74 Mus musculus sequence calculation
-
 6
3.1.3.74 Homo sapiens sequence calculation
-
 6