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Literature summary extracted from

  • Tarling, C.A.; He, S.; Sulzenbacher, G.; Bignon, C.; Bourne, Y.; Henrissat, B.; Withers, S.G.
    Identification of the catalytic nucleophile of the family 29 alpha-L-fucosidase from Thermotoga maritima through trapping of a covalent glycosyl-enzyme intermediate and mutagenesis (2003), J. Biol. Chem., 278, 47394-47399.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.51 azide increases the rate of substrate cleavage in depending of azide concentration Thermotoga maritima
3.2.1.51 formate increases the rate of substrate cleavage in depending of azide concentration Thermotoga maritima
3.2.1.51 methanol linear increase in enzyme-catalyzed cleavage of p-nitrophenyl alpha-L-fucoside and fucosyl fluoride Thermotoga maritima
3.2.1.51 NaCl small positive effect Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.51 D2224A the mutant shows a greatly reduced activity compared with wild-type enzyme Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.51 p-nitrophenyl alpha-L-fucoside substrate inhibition at high concentration Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 0.032
-
 p-nitrophenyl alpha-L-fucoside pH 5.0, 60°C Thermotoga maritima
3.2.1.51 0.034
-
 p-nitrophenyl alpha-L-fucoside pH 5.0, 37°C Thermotoga maritima
3.2.1.51 0.23
-
 alpha-L-fucosyl fluoride pH 5.0, 25°C Thermotoga maritima
3.2.1.51 2.1
-
 2-deoxy-2-fluoro-alpha-L-fucosyl fluoride pH 5.0, 60°C Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.51 additional information the addition of calcium, magnesium, cobalt, manganese, nickel, and zinc at 10 mM concentrations does not increase enzymatic rates Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Thermotoga maritima Q9WYE2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 2-deoxy-2-fluoro-alpha-L-fucosyl fluoride + H2O
-
 Thermotoga maritima fluoride + 2-deoxy-2-fluoro-alpha-L-fucose
-
 ?
3.2.1.51 alpha-L-fucosyl fluoride + H2O
-
 Thermotoga maritima alpha-L-fucose + fluoride
-
 ?
3.2.1.51 alpha-L-fucosyl fluoride + H2O
-
 Thermotoga maritima fluoride + alpha-L-fucose
-
 ?
3.2.1.51 p-nitrophenyl alpha-L-fucoside + H2O
-
 Thermotoga maritima p-nitrophenol + alpha-L-fucose
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.51 60
-
 the enzyme shows no loss of activity after 24 h Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.51 0.047
-
 2-deoxy-2-fluoro-alpha-L-fucosyl fluoride pH 5.0, 60°C Thermotoga maritima
3.2.1.51 2.9
-
 alpha-L-fucosyl fluoride pH 5.0, 25°C Thermotoga maritima
3.2.1.51 5.4
-
 p-nitrophenyl alpha-L-fucoside pH 5.0, 37°C Thermotoga maritima
3.2.1.51 9.1
-
 p-nitrophenyl alpha-L-fucoside pH 5.0, 60°C Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.51 4.3
-
-
 Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.51 3.7 5.5 less than 50% of maximal activity above and below Thermotoga maritima

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.51 3
-
 rapid inactivation Thermotoga maritima

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.51 0.64
-
 p-nitrophenyl alpha-L-fucoside pH 5.0, 60°C Thermotoga maritima