BRENDA - Enzyme Database

Analysis of the catalytic and binding residues of the diadenosine tetraphosphate pyrophosphohydrolase from Caenorhabditis elegans by site-directed mutagenesis

Abdelghany, H.M.; Bailey, S.; Blackburn, G.M.; Rafferty, J.B.; McLennan, A.G.; J. Biol. Chem. 278, 4435-4439 (2003)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.6.1.17
expression in Escherichia coli, mutant enzymes Y27A, Y27D, H31A, H31V, W32G, K36M, H38G, H38K, E52Q, E56Q, Y76A, Y76/Y121A, K79M, K81M, K83M, E103Q, Y121A
Caenorhabditis elegans
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.6.1.17
E103Q
turnover-number for hydrolysis of Ap4A is 3.2% of that of the wild-type enzyme, KM-value is 66% of the wild-type value
Caenorhabditis elegans
3.6.1.17
E52Q
turnover-number for hydrolysis of Ap4A is 0.02% of that of the wild-type enzyme, KM-value is 60% of the wild-type value
Caenorhabditis elegans
3.6.1.17
E56Q
turnover-number for hydrolysis of Ap4A is 0.001% of that of the wild-type enzyme, KM-value is 90% of the wild-type value
Caenorhabditis elegans
3.6.1.17
H31A
turnover-number for hydrolysis of Ap4A is 52% of that of the wild-type enzyme, KM-value is 8.3fold higher than the KM-value of the wild-type enzyme
Caenorhabditis elegans
3.6.1.17
H31V
turnover-number for hydrolysis of Ap4A is 61% of that of the wild-type enzyme, KM-value is 12.5fold higher than the KM-value of the wild-type enzyme
Caenorhabditis elegans
3.6.1.17
H38G
turnover-number for hydrolysis of Ap4A is 135% of that of the wild-type enzyme, KM-value is 66% of the wild-type value
Caenorhabditis elegans
3.6.1.17
H38K
turnover-number for hydrolysis of Ap4A is 135% of that of the wild-type enzyme, KM-value is 47% of the wild-type value
Caenorhabditis elegans
3.6.1.17
K36M
turnover-number for hydrolysis of Ap4A is 9% of that of the wild-type enzyme, KM-value is 1.5fold higher than the KM-value of the wild-type enzyme
Caenorhabditis elegans
3.6.1.17
K79M
turnover-number for hydrolysis of Ap4A is 0.7% of that of the wild-type enzyme, KM-value is 1.7fold higher than the KM-value of the wild-type enzyme
Caenorhabditis elegans
3.6.1.17
K81M
turnover-number for hydrolysis of Ap4A is 130% of that of the wild-type enzyme, KM-value is 2.3fold higher than the KM-value of the wild-type enzyme
Caenorhabditis elegans
3.6.1.17
K83M
turnover-number for hydrolysis of Ap4A is 2.6% of that of the wild-type enzyme, KM-value is 15.9fold higher than the KM-value of the wild-type enzyme
Caenorhabditis elegans
3.6.1.17
Y121A
turnover-number for hydrolysis of Ap4A is 61% of that of the wild-type enzyme, KM-value is 8.5fold higher than the KM-value of the wild-type enzyme
Caenorhabditis elegans
3.6.1.17
Y27A
turnover-number for hydrolysis of Ap4A is 113% of that of the wild-type enzyme, KM-value is 1.25fold higher than the KM-value of the wild-type enzyme
Caenorhabditis elegans
3.6.1.17
Y27D
turnover-number for hydrolysis of Ap4A is 126% of that of the wild-type enzyme, KM-value is 1.8fold higher than the KM-value of the wild-type enzyme
Caenorhabditis elegans
3.6.1.17
Y76A
turnover-number for hydrolysis of Ap4A is 8.7% of that of the wild-type enzyme, KM-value is 6.7fold higher than the KM-value of the wild-type enzyme
Caenorhabditis elegans
3.6.1.17
Y76A/Y121A
turnover-number for hydrolysis of Ap4A is 4.8% of that of the wild-type enzyme, KM-value is 3.75fold higher than the KM-value of the wild-type enzyme
Caenorhabditis elegans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.0041
-
Ap4A
mutant enzyme H38K
Caenorhabditis elegans
3.6.1.17
0.0053
-
Ap4A
mutant enzyme E52Q
Caenorhabditis elegans
3.6.1.17
0.0058
-
Ap4A
mutant enzyme E103Q; mutant enzyme H38G
Caenorhabditis elegans
3.6.1.17
0.0079
-
Ap4A
mutant enzyme E56Q
Caenorhabditis elegans
3.6.1.17
0.0088
-
Ap4A
wild-type enzyme
Caenorhabditis elegans
3.6.1.17
0.011
-
Ap4A
mutant enzyme Y27A
Caenorhabditis elegans
3.6.1.17
0.013
-
Ap4A
mutant enzyme K36M
Caenorhabditis elegans
3.6.1.17
0.015
-
Ap4A
mutant enzyme K79M
Caenorhabditis elegans
3.6.1.17
0.016
-
Ap4A
mutant enzyme Y27D
Caenorhabditis elegans
3.6.1.17
0.02
-
Ap4A
mutant enzyme K81M
Caenorhabditis elegans
3.6.1.17
0.033
-
Ap4A
mutant enzyme Y76A/Y121A
Caenorhabditis elegans
3.6.1.17
0.059
-
Ap4A
mutant enzyme Y76A
Caenorhabditis elegans
3.6.1.17
0.073
-
Ap4A
mutant enzyme H31A
Caenorhabditis elegans
3.6.1.17
0.075
-
Ap4A
mutant enzyme Y121A
Caenorhabditis elegans
3.6.1.17
0.11
-
Ap4A
mutant enzyme H31V
Caenorhabditis elegans
3.6.1.17
0.14
-
Ap4A
mutant enzyme K83M
Caenorhabditis elegans
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.17
Caenorhabditis elegans
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.17
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
-
656173
Caenorhabditis elegans
ATP + AMP
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.00024
-
Ap4A
mutant enzyme E56Q
Caenorhabditis elegans
3.6.1.17
0.0052
-
Ap4A
mutant enzyme E52Q
Caenorhabditis elegans
3.6.1.17
0.16
-
Ap4A
mutant enzyme K79M
Caenorhabditis elegans
3.6.1.17
0.6
-
Ap4A
mutant enzyme K83M
Caenorhabditis elegans
3.6.1.17
0.75
-
Ap4A
mutant enzyme E103Q
Caenorhabditis elegans
3.6.1.17
1.1
-
Ap4A
mutant enzyme Y76A/Y121A
Caenorhabditis elegans
3.6.1.17
2
-
Ap4A
mutant enzyme Y76A
Caenorhabditis elegans
3.6.1.17
2.1
-
Ap4A
mutant enzyme K36M
Caenorhabditis elegans
3.6.1.17
12
-
Ap4A
mutant enzyme H31A
Caenorhabditis elegans
3.6.1.17
14
-
Ap4A
mutant enzyme H31V; mutant enzyme Y121A
Caenorhabditis elegans
3.6.1.17
23
-
Ap4A
wild-type enzyme
Caenorhabditis elegans
3.6.1.17
26
-
Ap4A
mutant enzyme Y27A
Caenorhabditis elegans
3.6.1.17
29
-
Ap4A
mutant enzyme Y27D
Caenorhabditis elegans
3.6.1.17
30
-
Ap4A
mutant enzyme K81M
Caenorhabditis elegans
3.6.1.17
31
-
Ap4A
mutant enzyme H38G; mutant enzyme H38K
Caenorhabditis elegans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.17
expression in Escherichia coli, mutant enzymes Y27A, Y27D, H31A, H31V, W32G, K36M, H38G, H38K, E52Q, E56Q, Y76A, Y76/Y121A, K79M, K81M, K83M, E103Q, Y121A
Caenorhabditis elegans
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.6.1.17
E103Q
turnover-number for hydrolysis of Ap4A is 3.2% of that of the wild-type enzyme, KM-value is 66% of the wild-type value
Caenorhabditis elegans
3.6.1.17
E52Q
turnover-number for hydrolysis of Ap4A is 0.02% of that of the wild-type enzyme, KM-value is 60% of the wild-type value
Caenorhabditis elegans
3.6.1.17
E56Q
turnover-number for hydrolysis of Ap4A is 0.001% of that of the wild-type enzyme, KM-value is 90% of the wild-type value
Caenorhabditis elegans
3.6.1.17
H31A
turnover-number for hydrolysis of Ap4A is 52% of that of the wild-type enzyme, KM-value is 8.3fold higher than the KM-value of the wild-type enzyme
Caenorhabditis elegans
3.6.1.17
H31V
turnover-number for hydrolysis of Ap4A is 61% of that of the wild-type enzyme, KM-value is 12.5fold higher than the KM-value of the wild-type enzyme
Caenorhabditis elegans
3.6.1.17
H38G
turnover-number for hydrolysis of Ap4A is 135% of that of the wild-type enzyme, KM-value is 66% of the wild-type value
Caenorhabditis elegans
3.6.1.17
H38K
turnover-number for hydrolysis of Ap4A is 135% of that of the wild-type enzyme, KM-value is 47% of the wild-type value
Caenorhabditis elegans
3.6.1.17
K36M
turnover-number for hydrolysis of Ap4A is 9% of that of the wild-type enzyme, KM-value is 1.5fold higher than the KM-value of the wild-type enzyme
Caenorhabditis elegans
3.6.1.17
K79M
turnover-number for hydrolysis of Ap4A is 0.7% of that of the wild-type enzyme, KM-value is 1.7fold higher than the KM-value of the wild-type enzyme
Caenorhabditis elegans
3.6.1.17
K81M
turnover-number for hydrolysis of Ap4A is 130% of that of the wild-type enzyme, KM-value is 2.3fold higher than the KM-value of the wild-type enzyme
Caenorhabditis elegans
3.6.1.17
K83M
turnover-number for hydrolysis of Ap4A is 2.6% of that of the wild-type enzyme, KM-value is 15.9fold higher than the KM-value of the wild-type enzyme
Caenorhabditis elegans
3.6.1.17
Y121A
turnover-number for hydrolysis of Ap4A is 61% of that of the wild-type enzyme, KM-value is 8.5fold higher than the KM-value of the wild-type enzyme
Caenorhabditis elegans
3.6.1.17
Y27A
turnover-number for hydrolysis of Ap4A is 113% of that of the wild-type enzyme, KM-value is 1.25fold higher than the KM-value of the wild-type enzyme
Caenorhabditis elegans
3.6.1.17
Y27D
turnover-number for hydrolysis of Ap4A is 126% of that of the wild-type enzyme, KM-value is 1.8fold higher than the KM-value of the wild-type enzyme
Caenorhabditis elegans
3.6.1.17
Y76A
turnover-number for hydrolysis of Ap4A is 8.7% of that of the wild-type enzyme, KM-value is 6.7fold higher than the KM-value of the wild-type enzyme
Caenorhabditis elegans
3.6.1.17
Y76A/Y121A
turnover-number for hydrolysis of Ap4A is 4.8% of that of the wild-type enzyme, KM-value is 3.75fold higher than the KM-value of the wild-type enzyme
Caenorhabditis elegans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.0041
-
Ap4A
mutant enzyme H38K
Caenorhabditis elegans
3.6.1.17
0.0053
-
Ap4A
mutant enzyme E52Q
Caenorhabditis elegans
3.6.1.17
0.0058
-
Ap4A
mutant enzyme E103Q; mutant enzyme H38G
Caenorhabditis elegans
3.6.1.17
0.0079
-
Ap4A
mutant enzyme E56Q
Caenorhabditis elegans
3.6.1.17
0.0088
-
Ap4A
wild-type enzyme
Caenorhabditis elegans
3.6.1.17
0.011
-
Ap4A
mutant enzyme Y27A
Caenorhabditis elegans
3.6.1.17
0.013
-
Ap4A
mutant enzyme K36M
Caenorhabditis elegans
3.6.1.17
0.015
-
Ap4A
mutant enzyme K79M
Caenorhabditis elegans
3.6.1.17
0.016
-
Ap4A
mutant enzyme Y27D
Caenorhabditis elegans
3.6.1.17
0.02
-
Ap4A
mutant enzyme K81M
Caenorhabditis elegans
3.6.1.17
0.033
-
Ap4A
mutant enzyme Y76A/Y121A
Caenorhabditis elegans
3.6.1.17
0.059
-
Ap4A
mutant enzyme Y76A
Caenorhabditis elegans
3.6.1.17
0.073
-
Ap4A
mutant enzyme H31A
Caenorhabditis elegans
3.6.1.17
0.075
-
Ap4A
mutant enzyme Y121A
Caenorhabditis elegans
3.6.1.17
0.11
-
Ap4A
mutant enzyme H31V
Caenorhabditis elegans
3.6.1.17
0.14
-
Ap4A
mutant enzyme K83M
Caenorhabditis elegans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.17
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
-
656173
Caenorhabditis elegans
ATP + AMP
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.00024
-
Ap4A
mutant enzyme E56Q
Caenorhabditis elegans
3.6.1.17
0.0052
-
Ap4A
mutant enzyme E52Q
Caenorhabditis elegans
3.6.1.17
0.16
-
Ap4A
mutant enzyme K79M
Caenorhabditis elegans
3.6.1.17
0.6
-
Ap4A
mutant enzyme K83M
Caenorhabditis elegans
3.6.1.17
0.75
-
Ap4A
mutant enzyme E103Q
Caenorhabditis elegans
3.6.1.17
1.1
-
Ap4A
mutant enzyme Y76A/Y121A
Caenorhabditis elegans
3.6.1.17
2
-
Ap4A
mutant enzyme Y76A
Caenorhabditis elegans
3.6.1.17
2.1
-
Ap4A
mutant enzyme K36M
Caenorhabditis elegans
3.6.1.17
12
-
Ap4A
mutant enzyme H31A
Caenorhabditis elegans
3.6.1.17
14
-
Ap4A
mutant enzyme H31V; mutant enzyme Y121A
Caenorhabditis elegans
3.6.1.17
23
-
Ap4A
wild-type enzyme
Caenorhabditis elegans
3.6.1.17
26
-
Ap4A
mutant enzyme Y27A
Caenorhabditis elegans
3.6.1.17
29
-
Ap4A
mutant enzyme Y27D
Caenorhabditis elegans
3.6.1.17
30
-
Ap4A
mutant enzyme K81M
Caenorhabditis elegans
3.6.1.17
31
-
Ap4A
mutant enzyme H38G; mutant enzyme H38K
Caenorhabditis elegans