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Literature summary extracted from

  • Abdelghany, H.M.; Bailey, S.; Blackburn, G.M.; Rafferty, J.B.; McLennan, A.G.
    Analysis of the catalytic and binding residues of the diadenosine tetraphosphate pyrophosphohydrolase from Caenorhabditis elegans by site-directed mutagenesis (2003), J. Biol. Chem., 278, 4435-4439.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.17 expression in Escherichia coli, mutant enzymes Y27A, Y27D, H31A, H31V, W32G, K36M, H38G, H38K, E52Q, E56Q, Y76A, Y76/Y121A, K79M, K81M, K83M, E103Q, Y121A Caenorhabditis elegans

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.17 E103Q turnover-number for hydrolysis of Ap4A is 3.2% of that of the wild-type enzyme, KM-value is 66% of the wild-type value Caenorhabditis elegans
3.6.1.17 E52Q turnover-number for hydrolysis of Ap4A is 0.02% of that of the wild-type enzyme, KM-value is 60% of the wild-type value Caenorhabditis elegans
3.6.1.17 E56Q turnover-number for hydrolysis of Ap4A is 0.001% of that of the wild-type enzyme, KM-value is 90% of the wild-type value Caenorhabditis elegans
3.6.1.17 H31A turnover-number for hydrolysis of Ap4A is 52% of that of the wild-type enzyme, KM-value is 8.3fold higher than the KM-value of the wild-type enzyme Caenorhabditis elegans
3.6.1.17 H31V turnover-number for hydrolysis of Ap4A is 61% of that of the wild-type enzyme, KM-value is 12.5fold higher than the KM-value of the wild-type enzyme Caenorhabditis elegans
3.6.1.17 H38G turnover-number for hydrolysis of Ap4A is 135% of that of the wild-type enzyme, KM-value is 66% of the wild-type value Caenorhabditis elegans
3.6.1.17 H38K turnover-number for hydrolysis of Ap4A is 135% of that of the wild-type enzyme, KM-value is 47% of the wild-type value Caenorhabditis elegans
3.6.1.17 K36M turnover-number for hydrolysis of Ap4A is 9% of that of the wild-type enzyme, KM-value is 1.5fold higher than the KM-value of the wild-type enzyme Caenorhabditis elegans
3.6.1.17 K79M turnover-number for hydrolysis of Ap4A is 0.7% of that of the wild-type enzyme, KM-value is 1.7fold higher than the KM-value of the wild-type enzyme Caenorhabditis elegans
3.6.1.17 K81M turnover-number for hydrolysis of Ap4A is 130% of that of the wild-type enzyme, KM-value is 2.3fold higher than the KM-value of the wild-type enzyme Caenorhabditis elegans
3.6.1.17 K83M turnover-number for hydrolysis of Ap4A is 2.6% of that of the wild-type enzyme, KM-value is 15.9fold higher than the KM-value of the wild-type enzyme Caenorhabditis elegans
3.6.1.17 Y121A turnover-number for hydrolysis of Ap4A is 61% of that of the wild-type enzyme, KM-value is 8.5fold higher than the KM-value of the wild-type enzyme Caenorhabditis elegans
3.6.1.17 Y27A turnover-number for hydrolysis of Ap4A is 113% of that of the wild-type enzyme, KM-value is 1.25fold higher than the KM-value of the wild-type enzyme Caenorhabditis elegans
3.6.1.17 Y27D turnover-number for hydrolysis of Ap4A is 126% of that of the wild-type enzyme, KM-value is 1.8fold higher than the KM-value of the wild-type enzyme Caenorhabditis elegans
3.6.1.17 Y76A turnover-number for hydrolysis of Ap4A is 8.7% of that of the wild-type enzyme, KM-value is 6.7fold higher than the KM-value of the wild-type enzyme Caenorhabditis elegans
3.6.1.17 Y76A/Y121A turnover-number for hydrolysis of Ap4A is 4.8% of that of the wild-type enzyme, KM-value is 3.75fold higher than the KM-value of the wild-type enzyme Caenorhabditis elegans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.17 0.0041
-
 Ap4A mutant enzyme H38K Caenorhabditis elegans
3.6.1.17 0.0053
-
 Ap4A mutant enzyme E52Q Caenorhabditis elegans
3.6.1.17 0.0058
-
 Ap4A mutant enzyme E103Q Caenorhabditis elegans
3.6.1.17 0.0058
-
 Ap4A mutant enzyme H38G Caenorhabditis elegans
3.6.1.17 0.0079
-
 Ap4A mutant enzyme E56Q Caenorhabditis elegans
3.6.1.17 0.0088
-
 Ap4A wild-type enzyme Caenorhabditis elegans
3.6.1.17 0.011
-
 Ap4A mutant enzyme Y27A Caenorhabditis elegans
3.6.1.17 0.013
-
 Ap4A mutant enzyme K36M Caenorhabditis elegans
3.6.1.17 0.015
-
 Ap4A mutant enzyme K79M Caenorhabditis elegans
3.6.1.17 0.016
-
 Ap4A mutant enzyme Y27D Caenorhabditis elegans
3.6.1.17 0.02
-
 Ap4A mutant enzyme K81M Caenorhabditis elegans
3.6.1.17 0.033
-
 Ap4A mutant enzyme Y76A/Y121A Caenorhabditis elegans
3.6.1.17 0.059
-
 Ap4A mutant enzyme Y76A Caenorhabditis elegans
3.6.1.17 0.073
-
 Ap4A mutant enzyme H31A Caenorhabditis elegans
3.6.1.17 0.075
-
 Ap4A mutant enzyme Y121A Caenorhabditis elegans
3.6.1.17 0.11
-
 Ap4A mutant enzyme H31V Caenorhabditis elegans
3.6.1.17 0.14
-
 Ap4A mutant enzyme K83M Caenorhabditis elegans

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.17 Caenorhabditis elegans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.17 Ap4A + H2O
-
 Caenorhabditis elegans ?
-
 ?
3.6.1.17 diadenosine 5',5''-P1,P4-tetraphosphate + H2O
-
 Caenorhabditis elegans ATP + AMP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.17 diadenosine tetraphosphate pyrophosphohydrolase
-
 Caenorhabditis elegans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.17 0.00024
-
 Ap4A mutant enzyme E56Q Caenorhabditis elegans
3.6.1.17 0.0052
-
 Ap4A mutant enzyme E52Q Caenorhabditis elegans
3.6.1.17 0.16
-
 Ap4A mutant enzyme K79M Caenorhabditis elegans
3.6.1.17 0.6
-
 Ap4A mutant enzyme K83M Caenorhabditis elegans
3.6.1.17 0.75
-
 Ap4A mutant enzyme E103Q Caenorhabditis elegans
3.6.1.17 1.1
-
 Ap4A mutant enzyme Y76A/Y121A Caenorhabditis elegans
3.6.1.17 2
-
 Ap4A mutant enzyme Y76A Caenorhabditis elegans
3.6.1.17 2.1
-
 Ap4A mutant enzyme K36M Caenorhabditis elegans
3.6.1.17 12
-
 Ap4A mutant enzyme H31A Caenorhabditis elegans
3.6.1.17 14
-
 Ap4A mutant enzyme H31V Caenorhabditis elegans
3.6.1.17 14
-
 Ap4A mutant enzyme Y121A Caenorhabditis elegans
3.6.1.17 23
-
 Ap4A wild-type enzyme Caenorhabditis elegans
3.6.1.17 26
-
 Ap4A mutant enzyme Y27A Caenorhabditis elegans
3.6.1.17 29
-
 Ap4A mutant enzyme Y27D Caenorhabditis elegans
3.6.1.17 30
-
 Ap4A mutant enzyme K81M Caenorhabditis elegans
3.6.1.17 31
-
 Ap4A mutant enzyme H38G Caenorhabditis elegans
3.6.1.17 31
-
 Ap4A mutant enzyme H38K Caenorhabditis elegans