BRENDA - Enzyme Database

Regulation of dinucleoside polyphosphate pools by the YgdP and ApaH hydrolases Is essential for the ability of Salmonella enterica serovar Typhimurium to invade cultured mammalian cells

Ismail, T.M.; Hart, C.A.; McLennan, A.G.; J. Biol. Chem. 278, 32602-32607 (2003)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.6.1.41
expression in Escherichia coli of a His-tagged recombinant protein
Salmonella enterica
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.6.1.41
additional information
generation of null mutants by replacement of the gene encoding the enzyme with antibiotic resistance cassettes, the resulting STYA201 strain shows reduced ability to invade cultured mammalian cells
Salmonella enterica
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.41
0.014
-
P1,P6-bis(5'-adenosyl)hexaphosphate
reaction medium contains 0.1 mM CoCl2
Salmonella enterica
3.6.1.41
0.037
-
P1,P4-bis(5'-adenosyl) tetraphosphate
reaction medium contains 0.1 mM CoCl2
Salmonella enterica
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.41
Salmonella enterica
-
serovar typhimurium
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.41
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
hydrolysis of Ap4A
656153
Salmonella enterica
ADP
-
-
-
?
3.6.1.41
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O
hydrolysis of Ap6A
656153
Salmonella enterica
ADP + ?
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.41
expression in Escherichia coli of a His-tagged recombinant protein
Salmonella enterica
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.6.1.41
additional information
generation of null mutants by replacement of the gene encoding the enzyme with antibiotic resistance cassettes, the resulting STYA201 strain shows reduced ability to invade cultured mammalian cells
Salmonella enterica
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.41
0.014
-
P1,P6-bis(5'-adenosyl)hexaphosphate
reaction medium contains 0.1 mM CoCl2
Salmonella enterica
3.6.1.41
0.037
-
P1,P4-bis(5'-adenosyl) tetraphosphate
reaction medium contains 0.1 mM CoCl2
Salmonella enterica
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.41
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
hydrolysis of Ap4A
656153
Salmonella enterica
ADP
-
-
-
?
3.6.1.41
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O
hydrolysis of Ap6A
656153
Salmonella enterica
ADP + ?
-
-
-
?