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Literature summary extracted from

  • Mao, C.; Xu, R.; Szulc, Z.M.; Bielawski, J.; Becker, K.P.; Bielawska, A.; Galadari, S.H.; Hu, W.; Obeid, L.M.
    Cloning and characterization of a mouse endoplasmic reticulum alkaline ceramidase. An enzyme that preferentially regulates metabolism of very long chain ceramides (2003), J. Biol. Chem., 278, 31184-31191.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.23 maCER1 gene, overexpression in HeLa cells, expression in yeast cells and in COS-1 cells, sequencing Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.23 Cu2+ 1 mM, 60% inhibition, inhibits in a dose-dependent manner Mus musculus
3.5.1.23 Mn2+ 1 mM, 10% inhibition, inhibits in a dose-dependent manner Mus musculus
3.5.1.23 additional information not inhibited by Mg2+, phytosphingosine, dihydrosphingosine Mus musculus
3.5.1.23 sphingosine product inhibition in a dose-dependent manner, IC50: 0.08 mM Mus musculus
3.5.1.23 Zn2+ 1 mM, 60% inhibition, inhibits in a dose-dependent manner Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.23 endoplasmic reticulum maCER1 is localized to the endoplasmic reticulum and contains a Golgi to ER retrieval signal sequence Mus musculus 5783
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.23 Ca2+ slightly activates in a dose-dependent manner Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.23 additional information Mus musculus maCER1 may play a role in regulating the levels of bioactive lipids ceramide and sphingosine-1-phosphate, as well as complex sphingolipids, maCER1 may have a specific role in skin function ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.23 Mus musculus Q8R4X1
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.23 additional information very low expression of maCER1 in organs and tissues other than skin Mus musculus
-
3.5.1.23 skin maCER1 is predominantly and highly expressed in skin Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.23 D-erythro-C12-NBD-ceramide + H2O maCER1 has a highly restricted substrate specificity, maCER1 has a major ceramidase activity and only a very minor reverse activity Mus musculus D-erythro-sphingosine + 4-nitrobenzo-2-oxa-1,3-diazoyl-dodecanoic acid
-
 r
3.5.1.23 additional information maCER1 may play a role in regulating the levels of bioactive lipids ceramide and sphingosine-1-phosphate, as well as complex sphingolipids, maCER1 may have a specific role in skin function Mus musculus ?
-
 ?
3.5.1.23 additional information not: D-erythro-C12-NBD-dihydroceramide, D-ribo-C12-NBD-phytoceramide, L-threo-C12-NBD-ceramide Mus musculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.23 alkaline ceramidase
-
 Mus musculus
3.5.1.23 maCER1 mouse alkaline ceramidase Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.23 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.23 8
-
-
 Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.23 6 7.5 maCER1 has a slight activity in the range Mus musculus
3.5.1.23 6.5 10.5 maCER1 has a negligible activity at pH lower than 6.5 or higher than 10.5 Mus musculus
3.5.1.23 9.5 10 maCER1 has a slight activity in the range Mus musculus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.5.1.23 0.08
-
 product inhibition in a dose-dependent manner, IC50: 0.08 mM Mus musculus sphingosine