EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.B43 | - |
Niallia circulans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.B43 | 54 | - |
Keratan sulfate | keratan sulfate from cartilage, pH 6, 37°C | Niallia circulans | |
3.2.1.B43 | 143 | - |
Keratan sulfate | keratan sulfate from cornea, pH 6, 37°C | Niallia circulans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.B43 | 200000 | - |
SDS-PAGE and calculated from amino acid sequence | Niallia circulans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.B43 | Niallia circulans | Q7X0Z0 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.B43 | - |
Niallia circulans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.B43 | 1270 | - |
- |
Niallia circulans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.B43 | keratan sulfate + H2O | hydrolyzes keratan sulfate between the 4GlcNAcbeta1-3Gal1 structure, digests shark cartilage keratan sulfate to disaccharides and tetrasaccharides and bovine cornea keratan sulfate to hexasaccharides, prefers highly sulfated keratan sulfate | Niallia circulans | ? | - |
? | |
3.2.1.B43 | additional information | no substrates are: desulfated keratan sulfate, hyaluronic acid, chondroitin sulfate C, heparan sulfate, heparin | Niallia circulans | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.B43 | 55 | - |
- |
Niallia circulans |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.B43 | 50 | 60 | 80% activity at 50°C, 79% activity at 60°C | Niallia circulans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.B43 | 45 | 55 | stable up to 45°C, retains 65% activity at 50°C after 1 h at pH 6 in 100 mM acetate buffer, enzyme is unstable at 55°C | Niallia circulans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.B43 | 5 | - |
- |
Niallia circulans |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.B43 | 5 | 7 | enzyme is stable between pH 6-7, less stable at pH 5 | Niallia circulans |