Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yamagishi, K.; Suzuki, K.; Imai, K.; Mochizuki, H.; Morikawa, K.; Kyogashima, M.; Kimata, K.; Watanabe, H.
    Purification, characterization, and molecular cloning of a novel keratan sulfate hydrolase, endo-beta-N-acetylglucosaminidase, from Bacillus circulans (2003), J. Biol. Chem., 278, 25766-25772.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.B43
-
Niallia circulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B43 54
-
Keratan sulfate keratan sulfate from cartilage, pH 6, 37°C Niallia circulans
3.2.1.B43 143
-
Keratan sulfate keratan sulfate from cornea, pH 6, 37°C Niallia circulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.B43 200000
-
SDS-PAGE and calculated from amino acid sequence Niallia circulans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B43 Niallia circulans Q7X0Z0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.B43
-
Niallia circulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.B43 1270
-
-
Niallia circulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B43 keratan sulfate + H2O hydrolyzes keratan sulfate between the 4GlcNAcbeta1-3Gal1 structure, digests shark cartilage keratan sulfate to disaccharides and tetrasaccharides and bovine cornea keratan sulfate to hexasaccharides, prefers highly sulfated keratan sulfate Niallia circulans ?
-
?
3.2.1.B43 additional information no substrates are: desulfated keratan sulfate, hyaluronic acid, chondroitin sulfate C, heparan sulfate, heparin Niallia circulans ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B43 55
-
-
Niallia circulans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.B43 50 60 80% activity at 50°C, 79% activity at 60°C Niallia circulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.B43 45 55 stable up to 45°C, retains 65% activity at 50°C after 1 h at pH 6 in 100 mM acetate buffer, enzyme is unstable at 55°C Niallia circulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B43 5
-
-
Niallia circulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.B43 5 7 enzyme is stable between pH 6-7, less stable at pH 5 Niallia circulans