Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Reuland, S.N.; Vlasov, A.P.; Krupenko, S.A.
    Disruption of a calmodulin central helix-like region of 10-formyltetrahydrofolate dehydrogenase impairs its dehydrogenase activity by uncoupling the functional domains (2003), J. Biol. Chem., 278, 22894-22900.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.5.1.6 D399A 90% of the dehydrogenase activity of the wild-type enzyme Rattus norvegicus
1.5.1.6 E398A 90% of the dehydrogenase activity of the wild-type enzyme Rattus norvegicus
1.5.1.6 F384_V405del no dehydrogenase activity Rattus norvegicus
1.5.1.6 G397A as active as wild-type enzyme Rattus norvegicus
1.5.1.6 K394A 75% of the dehydrogenase activity of the wild-type enzyme Rattus norvegicus
1.5.1.6 K394A/L395A/R396A/G397A/E398A/D399A no dehydrogenase activity Rattus norvegicus
1.5.1.6 K394_D399del no dehydrogenase activity Rattus norvegicus
1.5.1.6 L395A 80% of the dehydrogenase activity of the wild-type enzyme Rattus norvegicus
1.5.1.6 R396A as active as wild-type enzyme Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.6 Rattus norvegicus P28037
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.6 intermediate domain and its mutants Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.5.1.6 -20°C, stable for several months Rattus norvegicus
1.5.1.6 4°C, stable for 2 weeks Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O the enzyme is composed of three domains and possesses three catalytic activities but has only two catalytic centers. The amino-terminal domain (residues 1-310) bears 10-formyltetrahydrofolate hydrolase activity, the carboxyl-terminal domain (residues 420-902) bears an aldehyde dehydrogenase activity, and the full-length FDH produces 10-formyltetrahydrofolate dehydrogenase activity Rattus norvegicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.6 10-formyltetrahydrofolate dehydrogenase
-
 Rattus norvegicus
1.5.1.6 FDH
-
 Rattus norvegicus