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Literature summary extracted from
- Mammalian fibroblasts lacking mitochondrial NAD+-dependent methylenetetrahydrofolate dehydrogenase-cyclohydrolase are glycine auxotrophs (2003), J. Biol. Chem., 278, 19436-19441.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.5.1.15 | additional information | primary fibroblasts from embryo of knock-out mice, spontaneous immortalization, the mutant cells require methionine and glycine for growth, glycine auxotrophy, replacement of mehtionine by homocysteine results in much lower enzyme activity, metabolism overview | Mus musculus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.5.1.15 | mitochondrion | - |
Mus musculus | 5739 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.15 | Mg2+ | required | Mus musculus |  |
| 1.5.1.15 | phosphate | required | Mus musculus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.1.15 | 5,10-methylenetetrahydrofolate + NAD+ | Mus musculus | - |
5,10-methenyltetrahydrofolate + NADH | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.1.15 | Mus musculus | - |
bifunctional enzyme | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.5.1.15 | embryo | enzyme is expressed during embryogenesis and in all immortilized cells | Mus musculus | - |
| 1.5.1.15 | fibroblast | primary embryonic | Mus musculus | - |
| 1.5.1.15 | additional information | cell growth studies, wild-type and mutant cell lines, overview | Mus musculus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.1.15 | 5,10-methylenetetrahydrofolate + NAD+ | - |
Mus musculus | 5,10-methenyltetrahydrofolate + NADH | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.5.1.15 | methylenetetrahydrofolate dehydrogenase-cyclohydrolase | - |
Mus musculus |
| 1.5.1.15 | methyleneTHF dehydrogenase | - |
Mus musculus |
| 1.5.1.15 | NMDMC | - |
Mus musculus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.15 | NAD+ | dependent on | Mus musculus | |
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Release 2023.1 (January 2023)
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