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Literature summary extracted from

  • Blonde, J.D.; Plaxton, W.C.
    Structural and kinetic properties of high and low molecular mass phosphoenolpyruvate carboxylase isoforms from the endosperm of developing castor oilseeds (2003), J. Biol. Chem., 278, 11867-11873.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.31 D-fructose 6-phosphate 2 mM, 1.2fold increase of activity of PEPC2 at pH 7.3, activity of PEPC2 at pH 8 is nearly identical to activity without glucose 6-phosphate Ricinus communis
4.1.1.31 D-glucose 1-phosphate 2 mM, 1.2fold increase of activity of PEPC2 at pH 7.3, activity of PEPC2 at pH 8 is nearly identical to activity without glucose 6-phosphate Ricinus communis
4.1.1.31 D-glucose 6-phosphate 2 mM, 2fold increase of activity of PEPC1 at pH 7.3, 1.18fold increase in activity of PEPC1 at pH 8 Ricinus communis
4.1.1.31 D-glucose 6-phosphate 2 mM, 1.2fold increase of activity of PEPC2 at pH 7.3, activity of PEPC2 at pH 8 is nearly identical to activity without glucose 6-phosphate Ricinus communis
4.1.1.31 fructose 6-phosphate 2 mM, 2fold increase of activity of PEPC1 at pH 7.3, 1.27fold increase in activity of PEPC1 at pH 8 Ricinus communis
4.1.1.31 glucose 1-phosphate 2 mM, 1.64fold increase of activity of PEPC1 at pH 7.3, 1.07fold increase in activity of PEPC1 at pH 8 Ricinus communis
4.1.1.31 glycerol 3-phosphate 2 mM, 1.83fold increase of activity of PEPC1 at pH 7.3, 1.16fold increase in activity of PEPC1 at pH 8 Ricinus communis
4.1.1.31 glycerol 3-phosphate 2 mM, 1.2fold increase of activity of PEPC2 at pH 7.3, activity of PEPC2 at pH 8 is nearly identical to activity without glucose 6-phosphate Ricinus communis
4.1.1.31 malate 2 mM, 1.2fold increase of activity of PEPC2 at pH 7.3, activity of PEPC2 at pH 8 is nearly identical to activity without glucose 6-phosphate Ricinus communis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.31 ATP 2 mM, 65% decreases of activity of PEPC1 at pH 7.3, 13% decrease in activity of PEPC2 at pH 8 Ricinus communis
4.1.1.31 L-Asp 2 mM, 95% decreases of activity of PEPC1 at pH 7.3, 49% decrease in activity of PEPC2 at pH 8 Ricinus communis
4.1.1.31 L-Glu 2 mM, 96% decreases of activity of PEPC1 at pH 7.3, 22% decrease in activity of PEPC2 at pH 8 Ricinus communis
4.1.1.31 malate 2 mM, 96% decreases of activity of PEPC1 at pH 7.3, 59% decrease in activity of PEPC1 at pH 8 Ricinus communis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.31 0.06
-
 phosphoenolpyruvate pH 8, PEPC1 Ricinus communis
4.1.1.31 0.06
-
 phosphoenolpyruvate pH 8, PEPC2 Ricinus communis
4.1.1.31 0.12
-
 phosphoenolpyruvate pH 7.3, PEPC1 Ricinus communis
4.1.1.31 0.12
-
 phosphoenolpyruvate pH 7.3, PEPC2 Ricinus communis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.31 64000
-
 4 * 107000 + 4 * 64000, PEPC2, SDS-PAGE Ricinus communis
4.1.1.31 107000
-
 4 * 107000 + 4 * 64000, PEPC2, SDS-PAGE Ricinus communis
4.1.1.31 107000
-
 4 * 107000, PEPC1, SDS-PAGE Ricinus communis
4.1.1.31 410000
-
 gel filtration, PEPC1 Ricinus communis
4.1.1.31 681000
-
 gel filtration, PEPC2 Ricinus communis

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.31 Ricinus communis
-
 L. Cv. Baker 296
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.31
-
 Ricinus communis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.31 24.2
-
 PEPC1 Ricinus communis
4.1.1.31 29.2
-
 PEPC2 Ricinus communis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.31 phosphoenolpyruvate + CO2
-
 Ricinus communis phosphate + oxaloacetate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.31 octamer 4 * 107000 + 4 * 64000, PEPC2, SDS-PAGE Ricinus communis
4.1.1.31 tetramer 4 * 107000, PEPC1, SDS-PAGE Ricinus communis

Synonyms

EC Number Synonyms Comment Organism
4.1.1.31 PEPC1
-
 Ricinus communis
4.1.1.31 PEPC2
-
 Ricinus communis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.31 30
-
 3 min, no loss of activity, PEPC1 Ricinus communis
4.1.1.31 35
-
 3 min, 19% loss of activity, PEPC1 Ricinus communis
4.1.1.31 40
-
 3 min, 25% loss of activity, PEPC1 Ricinus communis
4.1.1.31 45
-
 3 min, 40% loss of activity, PEPC1 Ricinus communis
4.1.1.31 45
-
 3 min, no loss of activity, PEPC2 Ricinus communis
4.1.1.31 50
-
 3 min, 82% loss of activity, PEPC1 Ricinus communis
4.1.1.31 50
-
 3 min, 20% loss of activity, PEPC2 Ricinus communis
4.1.1.31 55
-
 3 min, complete loss of activity, PEPC1 Ricinus communis
4.1.1.31 55
-
 3 min, complete loss of activity, PEPC2 Ricinus communis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.31 8
-
 PEPC2 Ricinus communis
4.1.1.31 8.5
-
 PEPC1 Ricinus communis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.31 6.5 9.5 pH 6.5: about 35% of maximal activity, pH 9.5: about 75% of maximal activity, PEPC2 Ricinus communis
4.1.1.31 7 9.6 pH 7.0: about 60% of maximal activity, pH 9.5: about 90% of maximal activity, PEPC1 Ricinus communis