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Literature summary extracted from

  • Jacob, R.; Peters, K.; Naim, H.Y.
    Prosequence of human lactase-phlorizin hydrolase modulates the folding of mature enzyme (2002), J. Biol. Chem., 277, 8217-8225.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.7.1.4 transient expression of mature enzyme and pro-enzyme in COS-1 cells, in vitro transcritption and translation Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.7.1.4 cytosol
-
 Homo sapiens 5829
-
3.7.1.4 endoplasmic reticulum the recombinant precusor in COS-1 cells resides in the ER, not mature wild-type Homo sapiens 5783
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.62 Homo sapiens
-
-
-
3.2.1.108 Homo sapiens
-
-
-
3.7.1.4 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.62 glycoprotein processing of enzyme to a complex glycosylated protein increases in presence of pro-region Homo sapiens
3.2.1.62 proteolytic modification pro-enzyme is cleaved in trans-Golgi network between Arg734 and Leu735, pro-region of enzyme is an intramolecular chaperone, analysis of processing and folding Homo sapiens
3.2.1.108 glycoprotein processing of enzyme to a complex glycosylated protein increases in presence of pro-region Homo sapiens
3.2.1.108 proteolytic modification pro-enzyme is cleaved in trans-Golgi network between Arg734 and Leu735, pro-region of enzyme is an intramolecular chaperone, analysis of processing and folding Homo sapiens
3.7.1.4 glycoprotein mannose-rich, complex glycosylated mature enzyme, dependent on ooptimal folding of the processed proform in presence of the pro-peptide Homo sapiens
3.7.1.4 proteolytic modification enzyme is sythesized as a transport-competent precursor, i.e. pro-LPH, intracellular cleavage process in the trans-Golgi network between Arg734 and Leu 735 to yield the mature enzyme, i.e. LPHbetainitial, the propeptide of the zymogen, i.e. LPHalpha, modulates as a chaperone the folding of the mature enzyme after it has been cleaved off, mechanism, overview Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.4 recombinant mature enzyme and pro-enzyme from COS-1 cells Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.7.1.4 phloretin + H2O = phloretate + phloroglucinol also hydrolyses other C-acetylated phenols related to phloretin Homo sapiens
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.7.1.4 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.4 lactose + H2O
-
 Homo sapiens beta-D-galactose + beta-D-glucose
-
 ?
3.7.1.4 additional information the enzyme interacts with ER chaperones, immunglobulin binding protein, and calnexin at non-permissive temperatures in the ER Homo sapiens ?
-
 ?
3.7.1.4 phloretin + H2O
-
 Homo sapiens phloretate + phloroglucinol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.7.1.4 More the mature enzyme shows a temperature-sensitive conformation, interaction with ER chaperones, immunglobulin binding protein, and calnexin, enzyme structure, overview Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.7.1.4 lactase-phlorizin hydrolase
-
 Homo sapiens
3.7.1.4 LPH
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.7.1.4 additional information
-
 the mature enzyme shows a temperature-sensitive conformation Homo sapiens