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Literature summary extracted from

  • Shallom, D.; Belakhov, V.; Solomon, D.; Shoham, G.; Baasov, T.; Shoham, Y.
    Detailed kinetic analysis and identification of the nucleophile in alpha-L-arabinofuranosidase from Geobacillus stearothermophilus T-6, a family 51 glycoside hydrolase (2002), J. Biol. Chem., 277, 43667-43673.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 overexpression of wild-type and mutant enzymes in Escherichia coli BL21(DE3) Geobacillus stearothermophilus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.55 E175A site-directed mutagenesis, altered kinetic properties Geobacillus stearothermophilus
3.2.1.55 E294A site-directed mutagenesis, reduced activity and about 10fold increased Km for substrate 2',4',6'-trichlorophenyl alpha-L-arabinofuranoside compared to the wild-type enzyme Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.55 3',4'-dinitrophenyl alpha-L-arabinofuranoside uncompetitive substrate inhibition occurs at high concentrations Geobacillus stearothermophilus
3.2.1.55 3-nitrophenyl-alpha-L-arabinofuranoside uncompetitive substrate inhibition occurs at high concentrations Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 additional information
-
 additional information detailed kinetic study, overview Geobacillus stearothermophilus
3.2.1.55 0.003
-
 2',5'-dinitrophenyl alpha-L-arabinofuranoside pH 6.0, 40°C, mutant E175A Geobacillus stearothermophilus
3.2.1.55 0.01
-
 2',4',6'-trichlorophenyl alpha-L-arabinofuranoside pH 6.0, 40°C, mutant E175A Geobacillus stearothermophilus
3.2.1.55 0.01
-
 2',4',6'-trichlorophenyl alpha-L-arabinofuranoside pH 6.0, 40°C, wild-type enzyme Geobacillus stearothermophilus
3.2.1.55 0.04
-
 2',4',6'-trichlorophenyl alpha-L-arabinofuranoside pH 6.0, 40°C, mutant E175A Geobacillus stearothermophilus
3.2.1.55 0.14
-
 4-nitrophenyl-alpha-L-arabinofuranoside pH 6.0, 40°C, mutant E175A Geobacillus stearothermophilus
3.2.1.55 0.35
-
 2',5'-dinitrophenyl alpha-L-arabinofuranoside pH 6.0, 40°C, wild-type enzyme Geobacillus stearothermophilus
3.2.1.55 0.53
-
 2',4',6'-trichlorophenyl alpha-L-arabinofuranoside pH 6.0, 40°C, wild-type enzyme Geobacillus stearothermophilus
3.2.1.55 0.6
-
 3-nitrophenyl-alpha-L-arabinofuranoside pH 6.0, 40°C, mutant E175A Geobacillus stearothermophilus
3.2.1.55 0.65
-
 4-nitrophenyl-alpha-L-arabinofuranoside pH 6.0, 40°C, wild-type enzyme Geobacillus stearothermophilus
3.2.1.55 1.7
-
 3-nitrophenyl-alpha-L-arabinofuranoside pH 6.0, 40°C, wild-type enzyme Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.55 Sodium azide activates the wild-type enzyme over 40fold Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.55 additional information Geobacillus stearothermophilus key enzyme in the complete degradation of the plant cell wall, enzyme is a hemicellulose, probable mechanistic pathway ?
-
 ?
3.2.1.55 additional information Geobacillus stearothermophilus T-6 key enzyme in the complete degradation of the plant cell wall, enzyme is a hemicellulose, probable mechanistic pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Geobacillus stearothermophilus Q9XBQ3 gene abfA
-
3.2.1.55 Geobacillus stearothermophilus T-6 Q9XBQ3 gene abfA
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.55 1,5-alpha-L-arabinofuranohexaose + 5 H2O = 6 alpha-L-arabinofuranose catalytic mechanism, Glu175 is the acid-base residue, Glu294 functions as the catalytic nucleophile Geobacillus stearothermophilus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 2',4',6'-trichlorophenyl alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus 2,4,6-trichlorophenol + alpha-L-arabinofuranose
-
 r
3.2.1.55 2',4',6'-trichlorophenyl alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus T-6 2,4,6-trichlorophenol + alpha-L-arabinofuranose
-
 r
3.2.1.55 2',4',6'-trichlorophenyl alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus 2',4',6'-trichlorophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.55 2',5'-dinitrophenyl alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus 2,5-dinitrophenol + alpha-L-arabinofuranose
-
 r
3.2.1.55 2',5'-dinitrophenyl alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus T-6 2,5-dinitrophenol + alpha-L-arabinofuranose
-
 r
3.2.1.55 2,5-dinitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus 2,5-dinitrophenol + L-arabinofuranose
-
 ?
3.2.1.55 3',4''-dinitrophenyl alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus 3',4''-dinitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.55 3',4'-dinitrophenyl alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus 3',4'-dinitrophenol + alpha-L-arabinofuranose
-
 r
3.2.1.55 3-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus 3-nitrophenol + alpha-L-arabinofuranose
-
 r
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus 4-nitrophenol + alpha-L-arabinofuranose
-
 r
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus T-6 4-nitrophenol + alpha-L-arabinofuranose
-
 r
3.2.1.55 m-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus m-nitrophenol + L-arabinofuranose
-
 ?
3.2.1.55 additional information key enzyme in the complete degradation of the plant cell wall, enzyme is a hemicellulose, probable mechanistic pathway Geobacillus stearothermophilus ?
-
 ?
3.2.1.55 additional information stereochemistry of the reversible hydrolytic reaction, enzyme performs glycosyl transfer to low molecular weight alcohols Geobacillus stearothermophilus ?
-
 ?
3.2.1.55 additional information key enzyme in the complete degradation of the plant cell wall, enzyme is a hemicellulose, probable mechanistic pathway Geobacillus stearothermophilus T-6 ?
-
 ?
3.2.1.55 additional information stereochemistry of the reversible hydrolytic reaction, enzyme performs glycosyl transfer to low molecular weight alcohols Geobacillus stearothermophilus T-6 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 alpha-L-arabinofuranosidase
-
 Geobacillus stearothermophilus
3.2.1.55 More enzyme belongs to the family 51 of glycoside hydrolases Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 40
-
 assay at Geobacillus stearothermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.55 40 70
-
 Geobacillus stearothermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.55 additional information
-
 additional information kcat with mutant 294A Geobacillus stearothermophilus
3.2.1.55 0.0041
-
 3-nitrophenyl-alpha-L-arabinofuranoside pH 6.0, 40°C, mutant E175A Geobacillus stearothermophilus
3.2.1.55 0.08
-
 4-nitrophenyl-alpha-L-arabinofuranoside pH 6.0, 40°C, mutant E175A Geobacillus stearothermophilus
3.2.1.55 0.24
-
 2',4',6'-trichlorophenyl alpha-L-arabinofuranoside pH 6.0, 40°C, mutant E175A Geobacillus stearothermophilus
3.2.1.55 0.66
-
 3',4'-dinitrophenyl alpha-L-arabinofuranoside pH 6.0, 40°C, mutant E175A Geobacillus stearothermophilus
3.2.1.55 0.72
-
 2',5'-dinitrophenyl alpha-L-arabinofuranoside pH 6.0, 40°C, mutant E175A Geobacillus stearothermophilus
3.2.1.55 65
-
 2',4',6'-trichlorophenyl alpha-L-arabinofuranoside pH 6.0, 40°C, wild-type enzyme Geobacillus stearothermophilus
3.2.1.55 83
-
 3-nitrophenyl-alpha-L-arabinofuranoside pH 6.0, 40°C, wild-type enzyme Geobacillus stearothermophilus
3.2.1.55 87
-
 4-nitrophenyl-alpha-L-arabinofuranoside pH 6.0, 40°C, wild-type enzyme Geobacillus stearothermophilus
3.2.1.55 190
-
 2',5'-dinitrophenyl alpha-L-arabinofuranoside pH 6.0, 40°C, wild-type enzyme Geobacillus stearothermophilus
3.2.1.55 340
-
 3',4'-dinitrophenyl alpha-L-arabinofuranoside pH 6.0, 40°C, wild-type enzyme Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 6
-
 assay at Geobacillus stearothermophilus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.55 10
-
 3-nitrophenyl-alpha-L-arabinofuranoside pH 6.0, 40°C, wild-type enzyme Geobacillus stearothermophilus
3.2.1.55 12
-
 3',4'-dinitrophenyl alpha-L-arabinofuranoside pH 6.0, 40°C, wild-type enzyme Geobacillus stearothermophilus