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Literature summary extracted from

  • Wang, Y.; Sun, D.; Davidson, V.L.
    Use of indirect site-directed mutagenesis to alter the substrate specificity of methylamine dehydrogenase (2002), J. Biol. Chem., 277, 4119-4122.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.4.9.1 alphaF55A 1656fold increase in Km-value for methylamine compared to wild-type enzyme, 484fold increase in Km-value for ethylamine compared to wild-type enzyme, 36fold increase in Km-value for propylamine compared to wild-type enzyme, 3.6fold decrease in Km-value for butylamine compared to wild-type enzyme, 53.2fold decrease in Km-value for 1-aminopentane compared to wild-type enzyme, 34.3fold decrease in Km-value for 1-6-diaminohexane compared to wild-type enzyme, 54.3fold decrease in Km-value for 1,7-diaminoheptane compared to wild-type enzyme Paracoccus denitrificans
1.4.9.1 alphaF55I 1.2fold decrease in Km-value for methylamine compared to wild-type enzyme, 18.9fold increase in Km-value for ethylamine compared to wild-type enzyme, 5.6fold increase in Km-value for propylamine compared to wild-type enzyme, 4.2fold decrease in Km-value for butylamine compared to wild-type enzyme, 10fold decrease in Km-value for 1-aminopentane compared to wild-type enzyme, 7.3fold decrease in Km-value for 1-6-diaminohexane compared to wild-type enzyme, 6.7fold decrease in Km-value for 1,7-diaminoheptane compared to wild-type enzym. Ability to discriminate between amines of different chain length is abolished Paracoccus denitrificans
1.4.9.1 betaI107N 27.8fold increase in Km-value for methylamine compared to wild-type enzyme, 44.2fold increase in Km-value for ethylamine compared to wild-type enzyme, 8.5fold decrease in Km-value for propylamine compared to wild-type enzyme, 124fold decrease in Km-value for butylamine compared to wild-type enzyme, 62.5fold decrease in Km-value for 1-aminopentane compared to wild-type enzyme, 23.2fold decrease in Km-value for 1-6-diaminohexane compared to wild-type enzyme, 5.6fold decrease in Km-value for 1,7-diaminoheptane compared to wild-type enzyme. Mutant enzyme exhibity a strong preference for 1-aminopentane compared to strong preference for methylamine of the wild-type enzyme Paracoccus denitrificans
1.4.9.1 betaI107V mutant enzyme exhibits a strong preference for propylamine compared to strong preference for methylamine of the wild-type enzyme. 7.7fold increase in Km-value for methylamine compared to wild-type enzyme, 17.9fold increase in Km-value for ethylamine compared to wild-type enzyme, 6fold decrease in Km-value for propylamine compared to wild-type enzyme, 9.9fold decrease in Km-value for butylamine compared to wild-type enzyme, 19.2fold decrease in Km-value for 1-aminopentane compared to wild-type enzyme, 4.2fold decrease in Km-value for 1-6-diaminohexane compared to wild-type enzyme, 1.3fold decrease in Km-value for 1,7-diaminoheptane compared to wild-type enzyme Paracoccus denitrificans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.9.1 0.004
-
 1-aminopentane pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
1.4.9.1 0.006
-
 Propylamine pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
1.4.9.1 0.007
-
 1,7-Diaminoheptane pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans
1.4.9.1 0.007
-
 Butylamine pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
1.4.9.1 0.008
-
 Propylamine pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
1.4.9.1 0.009
-
 methylamine pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
1.4.9.1 0.019
-
 ethylamine pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
1.4.9.1 0.021
-
 1,6-diaminohexane pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans
1.4.9.1 0.031
-
 1,6-diaminohexane pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
1.4.9.1 0.036
-
 Propylamine pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
1.4.9.1 0.047
-
 1-aminopentane pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans
1.4.9.1 0.057
-
 1,7-Diaminoheptane pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
1.4.9.1 0.06
-
 methylamine pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
1.4.9.1 0.068
-
 1,7-Diaminoheptane pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
1.4.9.1 0.069
-
 methylamine pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
1.4.9.1 0.088
-
 Butylamine pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
1.4.9.1 0.099
-
 1,6-diaminohexane pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
1.4.9.1 0.13
-
 1-aminopentane pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
1.4.9.1 0.17
-
 1,6-diaminohexane pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
1.4.9.1 0.2
-
 Propylamine pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
1.4.9.1 0.24
-
 Butylamine pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans
1.4.9.1 0.25
-
 1-aminopentane pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
1.4.9.1 0.25
-
 methylamine pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
1.4.9.1 0.29
-
 1,7-Diaminoheptane pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
1.4.9.1 0.34
-
 ethylamine pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
1.4.9.1 0.36
-
 ethylamine pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
1.4.9.1 0.37
-
 Butylamine pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
1.4.9.1 0.38
-
 1,7-Diaminoheptane pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
1.4.9.1 0.72
-
 1,6-diaminohexane pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
1.4.9.1 0.84
-
 ethylamine pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
1.4.9.1 0.87
-
 Butylamine pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
1.4.9.1 1.3
-
 Propylamine pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans
1.4.9.1 2.5
-
 1-aminopentane pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
1.4.9.1 9.2
-
 ethylamine pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans
1.4.9.1 14.9
-
 methylamine pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans

Organism

EC Number Organism UniProt Comment Textmining
1.4.9.1 Paracoccus denitrificans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.9.1 1,6-diaminohexane + acceptor + H2O acceptor: phenazine ethosulfate/2,6-dichlorophenolindophenol Paracoccus denitrificans ?
-
 ?
1.4.9.1 1,7-diaminoheptane + acceptor + H2O acceptor: phenazine ethosulfate/2,6-dichlorophenolindophenol Paracoccus denitrificans ?
-
 ?
1.4.9.1 1-aminopentane + acceptor + H2O acceptor: phenazine ethosulfate/2,6-dichlorophenolindophenol Paracoccus denitrificans pentanal + NH3 + reduced acceptor
-
 ?
1.4.9.1 butylamine + acceptor + H2O acceptor: phenazine ethosulfate/2,6-dichlorophenolindophenol Paracoccus denitrificans butanal + NH3 + reduced acceptor
-
 ?
1.4.9.1 ethylamine + acceptor + H2O acceptor: phenazine ethosulfate/2,6-dichlorophenolindophenol Paracoccus denitrificans acetaldehyde + NH3 + reduced acceptor
-
 ?
1.4.9.1 methylamine + acceptor + H2O acceptor: phenazine ethosulfate/2,6-dichlorophenolindophenol Paracoccus denitrificans formaldehyde + NH3 + reduced acceptor
-
 ?
1.4.9.1 propylamine + acceptor + H2O acceptor: phenazine ethosulfate/2,6-dichlorophenolindophenol Paracoccus denitrificans propionaldehyde + NH3 + reduced acceptor
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.9.1 2
-
 methylamine pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
1.4.9.1 3.1
-
 Propylamine pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
1.4.9.1 3.4
-
 1,6-diaminohexane pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
1.4.9.1 3.4
-
 1,7-Diaminoheptane pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
1.4.9.1 3.4
-
 1-aminopentane pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
1.4.9.1 4.1
-
 Propylamine pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
1.4.9.1 4.2
-
 Butylamine pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
1.4.9.1 4.2
-
 1-aminopentane pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
1.4.9.1 4.5
-
 ethylamine pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
1.4.9.1 5.2
-
 Propylamine pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
1.4.9.1 5.4
-
 Butylamine pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
1.4.9.1 5.4
-
 1-aminopentane pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
1.4.9.1 6.2
-
 ethylamine pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
1.4.9.1 14
-
 Butylamine pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
1.4.9.1 15
-
 ethylamine pH 7.5, 30°C, mutant enzyme alphaF55I Paracoccus denitrificans
1.4.9.1 15
-
 1,7-Diaminoheptane pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
1.4.9.1 16
-
 1,6-diaminohexane pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
1.4.9.1 17
-
 1,6-diaminohexane pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
1.4.9.1 17
-
 1-aminopentane pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
1.4.9.1 19
-
 1,7-Diaminoheptane pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
1.4.9.1 20
-
 1-aminopentane pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans
1.4.9.1 20
-
 methylamine pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
1.4.9.1 22
-
 Butylamine pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
1.4.9.1 23
-
 ethylamine pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans
1.4.9.1 24
-
 Propylamine pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans
1.4.9.1 24
-
 ethylamine pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
1.4.9.1 26
-
 1,6-diaminohexane pH 7.5, 30°C, mutant enzyme betaI107V Paracoccus denitrificans
1.4.9.1 27
-
 Propylamine pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
1.4.9.1 27
-
 1,7-Diaminoheptane pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
1.4.9.1 30
-
 methylamine pH 7.5, 30°C, wild-type enzyme Paracoccus denitrificans
1.4.9.1 32
-
 1,7-Diaminoheptane pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans
1.4.9.1 34
-
 Butylamine pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans
1.4.9.1 34
-
 methylamine pH 7.5, 30°C, mutant enzyme betaI107N Paracoccus denitrificans
1.4.9.1 43
-
 1,6-diaminohexane pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans
1.4.9.1 77
-
 methylamine pH 7.5, 30°C, mutant enzyme alphaF55A Paracoccus denitrificans