EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.20 | expression of chimeric mutant in an enzyme-deficient strain, overexpression of the plant enzyme from Arabidopsis thaliana in an enzyme-deficient strain can complement the mutant and results in 8fold increased accumulation of S-adenosyl-L-methionine, mechanism overview | Saccharomyces cerevisiae |
1.5.1.20 | overexpression of the wild-type enzyme and the chimeric mutant in a Saccharomyces cerevisiae enzyme-deficient strain, complementation of the enzyme-deficient strain, 8fold increased accumulation of S-adenosyl-L-methionine in case of recombinant wild-type expression in yeast, mechanism overview | Arabidopsis thaliana |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.5.1.20 | additional information | construction of a chimeric enzyme comprising the yeast N-terminal domain and the Arabidopsis thaliana C-terminal domain, recombinant expression in and complementation of an enzyme-deficient mutant strain with altered sensitivity to S-adenosyl-L-methionine and altered cofactor specifcity | Arabidopsis thaliana |
1.5.1.20 | additional information | construction of a chimeric enzyme comprising the yeast N-terminal domain and the Arabidopsis thaliana C-terminal domain, recombinant expression in and complementation of an enzyme-deficient mutant strain with altered sensitivity to S-adenosyl-L-methionine and altered cofactor specifcity | Saccharomyces cerevisiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.20 | 5,10-methylenetetrahydrofolate | substrate inhibition | Arabidopsis thaliana | |
1.5.1.20 | 5,10-methylenetetrahydrofolate | substrate inhibition | Saccharomyces cerevisiae | |
1.5.1.20 | additional information | no inhibition by S-adenosyl-L-methionine, which is no feed-back regulator of the methyl group biosynthesis pathway in plants | Arabidopsis thaliana | |
1.5.1.20 | S-adenosyl-L-methionine | feed-back regulation of the methyl group biosynthesis pathway in vivo, the chimeric mutant enzyme is insensitive to inhibition by S-adenosyl-L-methionine | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.20 | 0.0027 | - |
NADH | recombinant wild-type enzyme, pH 7.2 | Arabidopsis thaliana | |
1.5.1.20 | 0.0028 | - |
NADH | recombinant chimeric mutant enzyme, pH 7.2 | Arabidopsis thaliana | |
1.5.1.20 | 0.0028 | - |
NADH | recombinant chimeric mutant enzyme, pH 7.2 | Saccharomyces cerevisiae | |
1.5.1.20 | 0.0073 | - |
NADPH | recombinant chimeric mutant enzyme, pH 7.2 | Arabidopsis thaliana | |
1.5.1.20 | 0.0073 | - |
NADPH | recombinant chimeric mutant enzyme, pH 7.2 | Saccharomyces cerevisiae | |
1.5.1.20 | 0.011 | - |
5,10-methylenetetrahydrofolate | recombinant enzyme, with NADPH, pH 7.2 | Saccharomyces cerevisiae | |
1.5.1.20 | 0.021 | - |
NADPH | recombinant enzyme, pH 7.2 | Saccharomyces cerevisiae | |
1.5.1.20 | 0.106 | - |
5,10-methylenetetrahydrofolate | recombinant chimeric mutant enzyme, with NADH, pH 7.2 | Arabidopsis thaliana | |
1.5.1.20 | 0.106 | - |
5,10-methylenetetrahydrofolate | recombinant chimeric mutant enzyme, with NADH, pH 7.2 | Saccharomyces cerevisiae | |
1.5.1.20 | 0.152 | - |
5,10-methylenetetrahydrofolate | recombinant chimeric mutant enzyme, with NADPH, pH 7.2 | Arabidopsis thaliana | |
1.5.1.20 | 0.152 | - |
5,10-methylenetetrahydrofolate | recombinant chimeric mutant enzyme, with NADPH, pH 7.2 | Saccharomyces cerevisiae | |
1.5.1.20 | 0.287 | - |
5,10-methylenetetrahydrofolate | recombinant wild-type enzyme, with NADH, pH 7.2 | Arabidopsis thaliana |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.5.1.20 | cytosol | - |
Arabidopsis thaliana | 5829 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.20 | Arabidopsis thaliana | - |
gene met13 | - |
1.5.1.20 | Saccharomyces cerevisiae | P53128 | gene met13 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.20 | 5,10-methylenetetrahydrofolate + NAD(P)H | NADH is the preferred cofactor of the plant enzyme | Arabidopsis thaliana | 5-methyltetrahydrofolate + NAD(P)+ | - |
r | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + NADH + H+ | - |
Arabidopsis thaliana | 5-methyltetrahydrofolate + NAD+ | - |
r | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + NADH + H+ | - |
Saccharomyces cerevisiae | 5-methyltetrahydrofolate + NAD+ | - |
r | |
1.5.1.20 | 5,10-methylenetetrahydrofolate + NADPH | - |
Saccharomyces cerevisiae | 5-methyltetrahydrofolate + NADP+ | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.20 | AtMTHFR-1 | - |
Arabidopsis thaliana |
1.5.1.20 | methylenetetrahydrofolate reductase | - |
Arabidopsis thaliana |
1.5.1.20 | methylenetetrahydrofolate reductase | - |
Saccharomyces cerevisiae |
1.5.1.20 | MTHFR | - |
Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.20 | 7.2 | - |
assay at | Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.20 | additional information | wild-type shows no activity with NADH/NAD+ | Saccharomyces cerevisiae | |
1.5.1.20 | NAD+ | only active with the chimeric mutant enzyme, not with the wild-type | Saccharomyces cerevisiae | |
1.5.1.20 | NAD+ | recombinant wild-type and chimeric mutant enzymes | Arabidopsis thaliana | |
1.5.1.20 | NADH | only active with the chimeric mutant enzyme, not with the wild-type | Saccharomyces cerevisiae | |
1.5.1.20 | NADH | recombinant wild-type and chimeric mutant enzymes | Arabidopsis thaliana | |
1.5.1.20 | NADP+ | - |
Saccharomyces cerevisiae | |
1.5.1.20 | NADP+ | poor cofactor for the recombinant wild-type enzyme, but utilized by the chimeric mutant enzyme with activity equal to the activity with NAD+ | Arabidopsis thaliana | |
1.5.1.20 | NADPH | dependent on | Saccharomyces cerevisiae | |
1.5.1.20 | NADPH | poor cofactor for the recombinant wild-type enzyme, but utilized by the chimeric mutant enzyme with activity equal to the activity with NADH | Arabidopsis thaliana |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.20 | 0.455 | - |
5,10-methylenetetrahydrofolate | recombinant enzyme, with NADPH, pH 7.2 | Saccharomyces cerevisiae | |
1.5.1.20 | 0.873 | - |
5,10-methylenetetrahydrofolate | recombinant wild-type enzyme, with NADH, pH 7.2 | Arabidopsis thaliana |