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Literature summary extracted from
- ATPase activity of the MsbA lipid flippase of Escherichia coli (2002), J. Biol. Chem., 277, 36697-36705.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.6.2.1 | Kdo2-lipid A | - |
Escherichia coli |  |
| 7.6.2.1 | Phospholipids | The activity of purified MsbA is dependent upon the presence of phospholipids. | Escherichia coli | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 7.6.2.1 | the gene MsbA is cloned into pET28b behind the T7 promoter in-frame with an N-terminal His6tag | Escherichia coli |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 7.6.2.1 | A270T | mutant and wild-type enzymes have similar activities at 30°C, but the mutant activity is decreased significant at 42°C | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.6.2.1 | vanadate | maximal half-inhibition at 0.035 mM | Escherichia coli | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.6.2.1 | 0.878 | - |
ATP | pH 7.5, 37°C, KM value of purified, detergent-solubilized MsbA, whereas the KM value calculated in the presence of 0.05 mM KdO2-lipid A is decreased by more than half | Escherichia coli | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.6.2.1 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.6.2.1 | recombinant wild-type and mutant enzymes | Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 7.6.2.1 | 0.002 | 0.004 | activity of purified, detergent-solubilized MsbA | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.6.2.1 | ATP + H2O | - |
Escherichia coli | ADP + phosphate | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 7.6.2.1 | 7 | 8.5 | - |
Escherichia coli |
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