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Literature summary extracted from

  • Jedrzejas, M.J.; Mello, L.V.; de Groot, B.L.; Li, S.
    Mechanism of hyaluronan degradation by Streptococcus pneumoniae hyaluronate lyase. Structures of complexes with the substrate (2002), J. Biol. Chem., 277, 28287-28297.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.1 overexpression of mutant Y408F in Escherichia coli Streptococcus pneumoniae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.2.1 5 mg/ml Y408 mutant enzyme complexed with tetra- and hexasaccharide hyaluronan substrates, in 10 mM Tris-HCl, pH 7.4, 2 mM EDTA, 1 mM DL-dithiothreitol, against 100 mM sodium cacodylate, pH 6.0, 2.9 M ammonium sulfate, 5 mM EDTA, hanging drop vapour diffusion method, 0.002 ml equal volumes of protein and reservoir solution, 22°C, equilibration against 1 ml reservoir solution, several days, X-ray diffraction structure determination and analysis at 1.52-2.0 A resolution Streptococcus pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
4.2.2.1 Y408F enzyme substrate complex structure determination by crystallization Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.2.1 hyaluronan Streptococcus pneumoniae extracellular enzyme facilitates the invasion and spreading of the pathogenic bacterium in the host tissue by degrading the hosts' extracellular matrix component in connective tissue ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.1 Streptococcus pneumoniae
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.2.1 [hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate Asn349, His399, and Tyr408 are involved in catalysis, flexible, allosteric behavior of the enzyme, mechanism, active site structure and substrate binding and interactions, overview Streptococcus pneumoniae
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.1 additional information
-
 activity in different isolated strains from several sources, overview Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.1 hyaluronan extracellular enzyme facilitates the invasion and spreading of the pathogenic bacterium in the host tissue by degrading the hosts' extracellular matrix component in connective tissue Streptococcus pneumoniae ?
-
 ?
4.2.2.1 hyaluronan
-
 Streptococcus pneumoniae 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine unsaturated disaccharide units ?
4.2.2.1 hyaluronan hexasaccharide the longest segment that binds entirely within the active site, a cleft between alpha- and beta-domain Streptococcus pneumoniae 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine unsaturated disaccharide units ?
4.2.2.1 hyaluronan tetrasaccharide
-
 Streptococcus pneumoniae 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine unsaturated disaccharide units ?

Synonyms

EC Number Synonyms Comment Organism
4.2.2.1 spnHL
-
 Streptococcus pneumoniae