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Literature summary extracted from
- Enzymatic hydrolysis of pyridoxine-5'-beta-D-glucoside is catalyzed by intestinal lactase-phlorizin hydrolase (2002), J. Biol. Chem., 277, 26858-26864.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.62 | cellobiose | - |
Rattus norvegicus |  |
| 3.2.1.62 | D-glucose | - |
Rattus norvegicus | |
| 3.2.1.62 | lactose | competitive to pyridoxine-5'-beta-D-glucoside | Rattus norvegicus | |
| 3.2.1.62 | additional information | not inhibitory: pyridoxine, 2,4-dinitrophenyl-2-fluoro-2-deoxy-beta-D-glucopyranoside | Rattus norvegicus | |
| 3.2.1.62 | pyridoxine-5'-beta-D-glucoside | substrate inhibition above 3 mM | Rattus norvegicus | |
| 3.7.1.4 | 2',4'-dinitrophenyl-3-fluoro-2-deoxy-beta-D-glucopyranoside | specific 100fold inhibition of phloretin and lactose hydrolase activity, no inhibition of pyridoxine-5'-beta-D-glucoside hydrolase activity | Rattus norvegicus | |
| 3.7.1.4 | beta-D-glucose | inhibition of pyridoxine-5'-beta-D-glucoside hydrolase activity | Rattus norvegicus | |
| 3.7.1.4 | cellobiose | inhibition of pyridoxine-5'-beta-D-glucoside hydrolase activity | Rattus norvegicus | |
| 3.7.1.4 | lactose | inhibition of pyridoxine-5'-beta-D-glucoside hydrolase activity, competitive | Rattus norvegicus | |
| 3.7.1.4 | maltose | inhibition of pyridoxine-5'-beta-D-glucoside hydrolase activity, inhibition is reduced to 60-75% by addition of 1-deoxynojirimycin | Rattus norvegicus | |
| 3.7.1.4 | additional information | no inhibition by pyridoxine, trehalose, and sucrose | Rattus norvegicus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.62 | 1 | - |
pyridoxine-5'-beta-D-glucoside | pH 6.0, 37°C | Rattus norvegicus | |
| 3.2.1.62 | 16 | - |
lactose | pH 6.0, 37°C | Rattus norvegicus | |
| 3.7.1.4 | additional information | - |
additional information | kinetics | Rattus norvegicus | |
| 3.7.1.4 | 1 | - |
pyridoxine-5'-beta-D-glucoside | pH 6.0, 37°C | Rattus norvegicus | |
| 3.7.1.4 | 16 | - |
lactose | pH 6.0, 37°C | Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.62 | membrane | - |
Rattus norvegicus | 16020 | - |
| 3.7.1.4 | brush border | - |
Rattus norvegicus | 5903 | - |
| 3.7.1.4 | cytosol | - |
Rattus norvegicus | 5829 | - |
| 3.7.1.4 | membrane | - |
Rattus norvegicus | 16020 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.7.1.4 | phloretin + H2O | Rattus norvegicus | - |
phloretate + phloroglucinol | - |
? | |
| 3.7.1.4 | pyridoxine-5'-beta-D-glucoside + H2O | Rattus norvegicus | enzyme might play an important role in providing the bioavailability of pyridoxine-5'-beta-D-glucoside | pyridoxine + D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.62 | Rattus norvegicus | - |
- |
- |
| 3.7.1.4 | Rattus norvegicus | - |
dual specificity: phloretin hydrolase and pyridoxine-5'-beta-D-glucoside hydrolase activity | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.62 | proteolytic modification | precursor form of 200000-220000 Da | Rattus norvegicus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.62 | - |
Rattus norvegicus |
| 3.7.1.4 | 300fold from intestine brushh border membrane | Rattus norvegicus |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.7.1.4 | phloretin + H2O = phloretate + phloroglucinol | also hydrolyses other C-acetylated phenols related to phloretin | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.62 | small intestine | - |
Rattus norvegicus | - |
| 3.7.1.4 | mucosa | - |
Rattus norvegicus | - |
| 3.7.1.4 | small intestine | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.62 | lactose + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.2.1.62 | pyridoxine-5'-beta-D-glucoside + H2O | - |
Rattus norvegicus | pyridoxine + D-glucose | - |
r | |
| 3.7.1.4 | lactose + H2O | - |
Rattus norvegicus | beta-D-galactose + beta-D-glucose | - |
? | |
| 3.7.1.4 | additional information | the enzyme preparation also shows maltase-glucoamylase activity, EC 3.2.1.20, at 3.8 U/mg | Rattus norvegicus | ? | - |
? | |
| 3.7.1.4 | phloretin + H2O | - |
Rattus norvegicus | phloretate + phloroglucinol | - |
? | |
| 3.7.1.4 | pyridoxine-5'-beta-D-glucoside + H2O | - |
Rattus norvegicus | pyridoxine + D-glucose | - |
? | |
| 3.7.1.4 | pyridoxine-5'-beta-D-glucoside + H2O | enzyme might play an important role in providing the bioavailability of pyridoxine-5'-beta-D-glucoside | Rattus norvegicus | pyridoxine + D-glucose | - |
? | |
| 3.7.1.4 | pyridoxine-5'-beta-D-glucoside + lactose | - |
Rattus norvegicus | pyridoxine + beta-D-glucose + pyridoxine disaccharide | product identification, product is formed by a pyridoxine molecule with the 5'-beta-gkucoside and another glucose or galactose moiety | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.62 | ? | x * 135000-140000, SDS-PAGE, mature protein | Rattus norvegicus |
| 3.7.1.4 | ? | x * 135000-140000, SDS-PAGE | Rattus norvegicus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.7.1.4 | lactase phlorizin hydrolase | - |
Rattus norvegicus |
| 3.7.1.4 | lactase-phlorizin hydrolase | - |
Rattus norvegicus |
| 3.7.1.4 | LPH | - |
Rattus norvegicus |
| 3.7.1.4 | PNG hydrolase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.7.1.4 | 37 | - |
assay at | Rattus norvegicus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.62 | 1 | - |
pyridoxine-5'-beta-D-glucoside | pH 6.0, 37°C | Rattus norvegicus | |
| 3.7.1.4 | 1 | - |
pyridoxine-5'-beta-D-glucoside | pH 6.0, 37°C | Rattus norvegicus | |
| 3.7.1.4 | 47 | - |
lactose | pH 6.0, 37°C | Rattus norvegicus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.7.1.4 | 6 | - |
assay at | Rattus norvegicus |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.62 | 56 | - |
lactose | pH 6.0, 37°C | Rattus norvegicus | |
| 3.2.1.62 | 64 | - |
D-glucose | pH 6.0, 37°C | Rattus norvegicus | |
| 3.7.1.4 | 56 | - |
lactose | pH 6.0, 37°C | Rattus norvegicus | |
| 3.7.1.4 | 64 | - |
beta-D-glucose | pH 6.0, 37°C | Rattus norvegicus | |
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Release 2023.1 (January 2023)
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