EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.1 | expression of ADH4 in Escherichia coli | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.1 | all-trans-retinal | product inhibition | Homo sapiens | |
1.1.1.1 | all-trans-retinoic acid | weak feedback inhibition | Homo sapiens | |
1.1.1.1 | Isobutyramide | substrate inhibition, competitive against retinol, noncompetitive against NADH | Homo sapiens | |
1.1.1.1 | trifluoroethanol | competitive against retinol, noncompetitive against NAD+ | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.1 | additional information | - |
additional information | steady-state kinetics, kinetic mechanism | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.1 | all-trans-retinol + NAD+ | Homo sapiens | ADH4 might be involved in biosynthesis of retinoic acid | all-trans-retinal + NADH | - |
r | |
1.1.1.1 | isobutyramide + NAD+ | Homo sapiens | - |
? | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.1 | Homo sapiens | - |
class IV alcohol dehydrogenase | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.1 | recombinant enzyme from Escherichia coli by DEAE ion exchange, 5'-AMP-resin affinity, and Mono Q ion exchange chromatography | Homo sapiens |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.1 | a primary alcohol + NAD+ = an aldehyde + NADH + H+ | class IV alcohol dehydrogenase also functions as retinol dehydrogenase, reaction and kinetic mechanism: asymmetric rapid equilibrium random mechanism with 2 dead-end ternary complexes fro retinol oxidation and a rapid equilibrium ordered mechanism with one dead-end ternary complex for retinal reduction, a unique mechanistic form fro zinc-containing ADH in the medium chain dehydrogenase/reductase superfamily of enzymes | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.1 | all-trans-retinol + NAD+ | - |
Homo sapiens | all-trans-retinal + NADH | - |
r | |
1.1.1.1 | all-trans-retinol + NAD+ | ADH4 might be involved in biosynthesis of retinoic acid | Homo sapiens | all-trans-retinal + NADH | - |
r | |
1.1.1.1 | isobutyramide + NAD+ | - |
Homo sapiens | ? | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.1 | ADH4 | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.1 | 25 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.1 | 7.5 | - |
assay at | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.1 | NAD+ | - |
Homo sapiens | |
1.1.1.1 | NADH | - |
Homo sapiens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.1 | additional information | - |
additional information | - |
Homo sapiens |