BRENDA - Enzyme Database

Methanococcus jannaschii uses a pyruvoyl-dependent arginine decarboxylase in polyamine biosynthesis

Graham, D.E.; Xu, H.; White, R.H.; J. Biol. Chem. 277, 23500-23507 (2002)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
4.1.1.19
enzyme resists proteolytic cleavage by trypsin, pepsin, carboxypeptidase A, or papain
Methanocaldococcus jannaschii
4.1.1.19
enzyme retains full activity in presence of 1% w/v SDS at 70°C
Methanocaldococcus jannaschii
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.19
agmatine
1.4 mM, 50% inhibition
Methanocaldococcus jannaschii
4.1.1.19
guanidine
5-10 mM, 50% inhibition
Methanocaldococcus jannaschii
4.1.1.19
L-argininamide
5 mM, complete inhibition
Methanocaldococcus jannaschii
4.1.1.19
L-arginine methyl ester
5 mM, complete inhibition
Methanocaldococcus jannaschii
4.1.1.19
methylguanidine
5-10 mM, 50% inhibition
Methanocaldococcus jannaschii
4.1.1.19
Urea
4 M, 80% inhibition
Methanocaldococcus jannaschii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.19
4.7
-
L-Arg
pH 6.0, 70°C
Methanocaldococcus jannaschii
4.1.1.19
7.1
-
L-Arg
pH 6.0, 83°C
Methanocaldococcus jannaschii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.19
55900
-
gel filtration
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.1.19
L-arginine
Methanocaldococcus jannaschii
enzyme is involved in polyamine biosynthesis
agmatine + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.1.19
Methanocaldococcus jannaschii
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
4.1.1.19
proteolytic modification
the enzyme is formed by self-cleavage of a proenzyme into a 5000 Da subunit and a 12000 Da subunit that contains a reactive pyruvoyl group
Methanocaldococcus jannaschii
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.1.19
2.1
-
-
Methanocaldococcus jannaschii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.19
L-arginine
-
656053
Methanocaldococcus jannaschii
agmatine + CO2
-
-
-
?
4.1.1.19
L-arginine
enzyme is involved in polyamine biosynthesis
656053
Methanocaldococcus jannaschii
agmatine + CO2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.1.1.19
hexamer
3 * alpha 11000-13000 + 3 * beta 5000-7000, SDS-PAGE
Methanocaldococcus jannaschii
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.1.19
90
-
activity increases up to
Methanocaldococcus jannaschii
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.1.19
121
-
20 min, 50% loss of activity
Methanocaldococcus jannaschii
4.1.1.19
125
-
30 min, 84% loss of activity
Methanocaldococcus jannaschii
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.1.1.19
2.7
-
L-Arg
pH 6.0, 83°C
Methanocaldococcus jannaschii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.19
6
-
-
Methanocaldococcus jannaschii
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.1.19
5
8
pH 5.0: about 60% of maximal activity, pH 8.0: about 60% of maximal activity
Methanocaldococcus jannaschii
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
4.1.1.19
additional information
the enzyme contains a reactive pyruvoyl group
Methanocaldococcus jannaschii
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
4.1.1.19
additional information
the enzyme contains a reactive pyruvoyl group
Methanocaldococcus jannaschii
General Stability (protein specific)
EC Number
General Stability
Organism
4.1.1.19
enzyme resists proteolytic cleavage by trypsin, pepsin, carboxypeptidase A, or papain
Methanocaldococcus jannaschii
4.1.1.19
enzyme retains full activity in presence of 1% w/v SDS at 70°C
Methanocaldococcus jannaschii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.19
agmatine
1.4 mM, 50% inhibition
Methanocaldococcus jannaschii
4.1.1.19
guanidine
5-10 mM, 50% inhibition
Methanocaldococcus jannaschii
4.1.1.19
L-argininamide
5 mM, complete inhibition
Methanocaldococcus jannaschii
4.1.1.19
L-arginine methyl ester
5 mM, complete inhibition
Methanocaldococcus jannaschii
4.1.1.19
methylguanidine
5-10 mM, 50% inhibition
Methanocaldococcus jannaschii
4.1.1.19
Urea
4 M, 80% inhibition
Methanocaldococcus jannaschii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.19
4.7
-
L-Arg
pH 6.0, 70°C
Methanocaldococcus jannaschii
4.1.1.19
7.1
-
L-Arg
pH 6.0, 83°C
Methanocaldococcus jannaschii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.1.19
55900
-
gel filtration
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.1.19
L-arginine
Methanocaldococcus jannaschii
enzyme is involved in polyamine biosynthesis
agmatine + CO2
-
-
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
4.1.1.19
proteolytic modification
the enzyme is formed by self-cleavage of a proenzyme into a 5000 Da subunit and a 12000 Da subunit that contains a reactive pyruvoyl group
Methanocaldococcus jannaschii
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.1.19
2.1
-
-
Methanocaldococcus jannaschii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.19
L-arginine
-
656053
Methanocaldococcus jannaschii
agmatine + CO2
-
-
-
?
4.1.1.19
L-arginine
enzyme is involved in polyamine biosynthesis
656053
Methanocaldococcus jannaschii
agmatine + CO2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.1.19
hexamer
3 * alpha 11000-13000 + 3 * beta 5000-7000, SDS-PAGE
Methanocaldococcus jannaschii
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.1.19
90
-
activity increases up to
Methanocaldococcus jannaschii
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.1.19
121
-
20 min, 50% loss of activity
Methanocaldococcus jannaschii
4.1.1.19
125
-
30 min, 84% loss of activity
Methanocaldococcus jannaschii
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.1.1.19
2.7
-
L-Arg
pH 6.0, 83°C
Methanocaldococcus jannaschii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.19
6
-
-
Methanocaldococcus jannaschii
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.1.19
5
8
pH 5.0: about 60% of maximal activity, pH 8.0: about 60% of maximal activity
Methanocaldococcus jannaschii