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Literature summary extracted from

  • Nakanishi, Y.; Saijo, T.; Wada, Y.; Maeshima, M.
    Mutagenic analysis of functional residues in putative substrate-binding site and acidic domains of vacuolar H+-pyrophosphatase (2001), J. Biol. Chem., 276, 7654-7660.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.1.3.1 KCl 3.5fold increase at 50 mM Vigna radiata
7.1.3.1 additional information no activation with NaCl at 50 mM Vigna radiata

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.3.1 expressed in Saccharomyces cerevisiae BJ5458 Vigna radiata

Protein Variants

EC Number Protein Variants Comment Organism
7.1.3.1 C304R complete loss of activity, no proton pumping Vigna radiata
7.1.3.1 D279A complete loss of activity, no proton pumping Vigna radiata
7.1.3.1 D279E complete loss of activity, no proton pumping Vigna radiata
7.1.3.1 D283A complete loss of activity, no proton pumping Vigna radiata
7.1.3.1 D283E complete loss of activity, no proton pumping Vigna radiata
7.1.3.1 D287A complete loss of activity, no proton pumping Vigna radiata
7.1.3.1 D287E complete loss of activity, no proton pumping Vigna radiata
7.1.3.1 D723A complete loss of activity, no proton pumping Vigna radiata
7.1.3.1 D723E complete loss of activity, no proton pumping Vigna radiata
7.1.3.1 D727A complete loss of activity, no proton pumping Vigna radiata
7.1.3.1 D727E complete loss of activity, no proton pumping Vigna radiata
7.1.3.1 D731A complete loss of activity, no proton pumping Vigna radiata
7.1.3.1 D731E complete loss of activity, no proton pumping Vigna radiata
7.1.3.1 E263D 50% of hydrolytic activity, no proton pumping Vigna radiata
7.1.3.1 E263G complete loss of activity, no proton pumping Vigna radiata
7.1.3.1 K261R 25% of hydrolytic activity, no proton pumping Vigna radiata
7.1.3.1 V259A 60% of hydrolytic activity, no proton pumping Vigna radiata

General Stability

EC Number General Stability Organism
7.1.3.1 Mg2+ partially stabilizes enzyme against tryptic digestion Vigna radiata

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.3.1 additional information insensitive to KF Vigna radiata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.3.1 vacuolar membrane
-
 Vigna radiata 5774
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.3.1 Mg2+ required for activity and stability Vigna radiata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.3.1 73000
-
 SDS-PAGE Vigna radiata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.1.3.1 diphosphate + H2O + H+[side 1] Vigna radiata important for energy metabolism, provides energy for biosynthetic reactions, reaction coupled with proton pumping through the vacuole membrane 2 phosphate + H+[side 2]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.1.3.1 Vigna radiata
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.3.1 diphosphate + H2O + H+[side 1]
-
 Vigna radiata 2 phosphate + H+[side 2]
-
 ?
7.1.3.1 diphosphate + H2O + H+[side 1] important for energy metabolism, provides energy for biosynthetic reactions, reaction coupled with proton pumping through the vacuole membrane Vigna radiata 2 phosphate + H+[side 2]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.1.3.1 H+-translocating inorganic pyrophosphatase
-
 Vigna radiata