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Literature summary extracted from

  • Suzuki, H.; Nakayama, T.; Yonekura-Sakakibara, K.; Fukui, Y.; Nakamura, N.; Nakao, M.; Tanaka, Y.; Yamaguchi, M.A.; Kusumi, T.; Nishino, T.
    Malonyl-CoA:anthocyanin 5-O-glucoside-6"'-O-malonyltransferase from scarlet sage (Salvia splendens) flowers. Enzyme purification, gene cloning, expression, and characterization (2001), J. Biol. Chem., 276, 49013-49019.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.172 agriculture Ss5MaT1 cDNA may be an important tool for controlling flower colors by metabolic engineering of anthocyanin biosynthesis Salvia splendens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.172 from petals, expression in Escherichia coli JM109, sequencing Salvia splendens

General Stability

EC Number General Stability Organism
2.3.1.172 2-mercaptoethanol stabilizes during purification Salvia splendens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.172 acetyl-CoA 1 mM, 48% inhibition Salvia splendens
2.3.1.172 caffeic acid 10 mM, complete inhibition Salvia splendens
2.3.1.172 Cu2+ 0.1 mM, complete inhibition Salvia splendens
2.3.1.172 diethyldicarbonate 1 mM, 51% inhibition, 20 min, 20°C Salvia splendens
2.3.1.172 Hg2+ 0.1 mM, complete inhibition Salvia splendens
2.3.1.172 additional information not inhibited by Ca2+, Cd2+, Co2+, Fe2+, Mg2+, Mn2+, Ni2+, Sn2+, Zn2+, all at 0.1 mM, 1 mM dithiothreitol, 1 mM EDTA, 1% Tween 20, 1% Triton X-100, 0.1% CHAPS, 0.1 mM pelargonidin 3,5-diglucoside, 10 mM glucose Salvia splendens
2.3.1.172 N-ethylmaleimide 5 mM, 53% inhibition, 20 min, 20°C Salvia splendens
2.3.1.172 p-coumaric acid competitive inhibitor with respect to shisonin, 10 mM, complete inhibition Salvia splendens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.172 0.0141
-
 malonyl-CoA pH 7, 30°C, shisonin as cosubstrate, native Ss5MaT1 Salvia splendens
2.3.1.172 0.0219
-
 malonyl-CoA pH 7, 30°C, shisonin as cosubstrate, recombinant Ss5MaT1 Salvia splendens
2.3.1.172 0.0355
-
 shisonin pH 7, 30°C, malonyl-CoA as cosubstrate, recombinant Ss5MaT1 Salvia splendens
2.3.1.172 0.0402
-
 shisonin pH 7, 30°C, malonyl-CoA as cosubstrate, native Ss5MaT1 Salvia splendens
2.3.1.172 0.057
-
 malonyl-CoA pH 7, 30°C, bisdemalonylsalvianin as cosubstrate Salvia splendens
2.3.1.172 0.101
-
 pelargonidin 3-O-(6-caffeoyl-beta-D-glucoside) 5-O-beta-D-glucoside pH 7, 30°C, malonyl-CoA as cosubstrate Salvia splendens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.172 cytosol presumably located in Salvia splendens 5829
-
2.3.1.172 soluble recombinant Ss5MaT1 Salvia splendens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.172 46000
-
 gel filtration Salvia splendens
2.3.1.172 50000
-
 1 * 50000, SDS-PAGE, 1 * 50723, sequence calculation Salvia splendens
2.3.1.172 50723
-
 1 * 50000, SDS-PAGE, 1 * 50723, sequence calculation Salvia splendens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.172 additional information Salvia splendens Ss5MaT1 expression is temporally regulated during the color development of the flower, Ss5MaT1 plays a role during the coloration of the flowers, salvianin biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.172 Salvia splendens Q8W1W9
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.3.1.172 extremely unstable to oxidation Salvia splendens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.172 native Ss5MaT1 from flowers: 685fold, recombinant Ss5MaT1 Salvia splendens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.172 malonyl-CoA + pelargonidin 3-O-(6-caffeoyl-beta-D-glucoside) 5-O-beta-D-glucoside = CoA + 4'''-demalonylsalvianin specific for the penultimate step in salvianin biosynthesis, the enzyme also catalyses the malonylation of shisonin to malonylshisonin [cyanidin 3-O-(6''-O-p-coumaryl-beta-D-glucoside)-5-(6'''-O-malonyl-beta-D-glucoside)], the compounds 4'''-demalonylsalvianin, salvianin, pelargonidin 3,5-diglucoside and delphinidin 3,5-diglucoside cannot act as substrates Salvia splendens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.172 bract Ss5MaT1 mRNA is present at a lower level than in petals and sepals Salvia splendens
-
2.3.1.172 flower recently opened flowers, less than 2 cm in length, Ss5MaT1 mRNA is mainly accumulated in young to opening petals and sepals Salvia splendens
-
2.3.1.172 petal Ss5MaT1 mRNA is mainly accumulated in young to opening petals and sepals Salvia splendens
-
2.3.1.172 sepal Ss5MaT1 mRNA is mainly accumulated in young to opening petals and sepals Salvia splendens
-
2.3.1.172 stem red stems, Ss5MaT1 mRNA is present at a lower level than in petals and sepals Salvia splendens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.172 5.17
-
 pH 7, 30°C Salvia splendens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.172 acetyl-CoA + shisonin 2% of the activity with malonyl-CoA as acyl donor Salvia splendens CoA + acetylshisonin
-
 ?
2.3.1.172 malonyl-CoA + pelargonidin 3-O-(6-caffeoyl-beta-D-glucoside) 5-O-beta-D-glucoside Ss5MaT1 catalyzes the regiospecific transfer of the malonyl group from malonyl-CoA to the 6'''-hydroxyl group of the 5-glucosyl moiety of anthocyanins, e.g. bisdemalonylsalvianin, the presence of an aromatic acyl group at the 3-glucosidic moiety of anthocyanin is important for substrate recognition, strong acyl donor preference for malonyl-CoA Salvia splendens CoA + 4'''-demalonylsalvianin
-
 ?
2.3.1.172 malonyl-CoA + shisonin Ss5MaT1 catalyzes the regiospecific transfer of the malonyl group from malonyl-CoA to the 6'''-hydroxyl group of the 5-glucosyl moiety of shisonin, enzyme catalyzes exclusively the monomalonylation of shisonin, strong acyl donor preference for malonyl-CoA Salvia splendens CoA + 6'''-malonylshisonin
-
 ?
2.3.1.172 methylmalonyl-CoA + shisonin 3% of the activity with malonyl-CoA as acyl donor Salvia splendens CoA + methylmalonylshisonin
-
 ?
2.3.1.172 additional information Ss5MaT1 expression is temporally regulated during the color development of the flower, Ss5MaT1 plays a role during the coloration of the flowers, salvianin biosynthesis Salvia splendens ?
-
 ?
2.3.1.172 additional information not: 4'''-monodemalonylsalvianin, salvianin, pelargonidin 3,5-diglucoside, delphinidin 3,5-diglucoside, caffeoyl-CoA, p-coumaroyl-CoA, the second malonylation of 4'''-demalonylsalvianin to salvianin is catalyzed by a distinct malonyltransferase Salvia splendens ?
-
 ?
2.3.1.172 succinyl-CoA + shisonin 49% of the activity with malonyl-CoA as acyl donor Salvia splendens CoA + succinylshisonin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.172 monomer 1 * 50000, SDS-PAGE, 1 * 50723, sequence calculation Salvia splendens

Synonyms

EC Number Synonyms Comment Organism
2.3.1.172 5-O-glucoside-6'''-O-malonyltransferase
-
 Salvia splendens
2.3.1.172 anthocyanidin 5-glucoside malonyltransferase
-
 Salvia splendens
2.3.1.172 anthocyanidin 5-glycoside malonyltransferase
-
 Salvia splendens
2.3.1.172 anthocyanin 5-glycoside malonyltransferase
-
 Salvia splendens
2.3.1.172 malonyl-CoA:anthocyanin
-
 Salvia splendens
2.3.1.172 malonyl-CoA:anthocyanin 5-O-glucoside-6"'-O-malonyltransferase
-
 Salvia splendens
2.3.1.172 malonyltransferase, anthocyanidin 5-glycoside
-
 Salvia splendens
2.3.1.172 More member of the versatile plant acyltransferase family Salvia splendens
2.3.1.172 Ss5MaT1
-
 Salvia splendens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.172 30
-
 assay at Salvia splendens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.172 30
-
 pH 7, 20 min, stable below 30°C Salvia splendens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.172 4.3
-
 shisonin pH 7, 30°C, malonyl-CoA as cosubstrate, native Ss5MaT1 Salvia splendens
2.3.1.172 7.8
-
 pelargonidin 3-O-(6-caffeoyl-beta-D-glucoside) 5-O-beta-D-glucoside pH 7, 30°C, malonyl-CoA as cosubstrate Salvia splendens
2.3.1.172 8.7
-
 shisonin pH 7, 30°C, malonyl-CoA as cosubstrate, recombinant Ss5MaT1 Salvia splendens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.172 8
-
-
 Salvia splendens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.172 6 11 active in the range Salvia splendens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.1.172 7.5
-
 20°C, 20 h, stable Salvia splendens