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Literature summary extracted from

  • Li, S.; Jedrzejas, M.J.
    Hyaluronan binding and degradation by Streptococcus agalactiae hyaluronate lyase (2001), J. Biol. Chem., 276, 41407-41416.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.2.1 purified free enzyme and enzyme-product complex, X-ray diffraction structure determination and analysis at 2.1-2.2 A resolution Streptococcus agalactiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.2.1 additional information Streptococcus agalactiae enzyme is a virulence factor that helps the pathogen to brake through the biophysical barrier of the host tissues ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.1 Streptococcus agalactiae Q53591 gene hylB3502, strain 3502
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.2.1 proteolytic modification activation probably by autolytic cleavage Streptococcus agalactiae

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.2.1 [hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate substrate binding, structure and mechanims, and reaction mechanism, catalytic residues are His479, Tyr488, and Asn429, modeling of binding of a tetrasaccharide in the active site cleft Streptococcus agalactiae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.1 chondroitin sulfate degrades progressively unsulfated and 6-sulfated regions from the reducing end Streptococcus agalactiae ?
-
 ?
4.2.2.1 hyaluronan progressive degradation from the reducing end Streptococcus agalactiae 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
-
 ?
4.2.2.1 additional information enzyme is a virulence factor that helps the pathogen to brake through the biophysical barrier of the host tissues Streptococcus agalactiae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.1 ? 3 different form of 118, 111, and 92 kDa, the 118 kDa form is inactive Streptococcus agalactiae

Synonyms

EC Number Synonyms Comment Organism
4.2.2.1 SagHL
-
 Streptococcus agalactiae