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Literature summary extracted from

  • Bhagwat, M.; Nossal, N.G.
    Bacteriophage T4 RNase H removes both RNA primers and adjacent DNA from the 5' end of lagging strand fragments (2001), J. Biol. Chem., 276, 28516-28524.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.26.4 RNA-DNA*DNA hybrid + H2O Tequatrovirus T4 enzyme removes RNA primers from lagging strand fragments during DNA replication, 5'-to 3'-exonuclease activity, degradation of the RNA portion of the duplex DNA*DNA + 5'-phosphomononucleotides
-
 ?
3.1.26.4 ssDNA-dsDNA + H2O Tequatrovirus T4 5'-to 3'-exonuclease activity, exonuclease activity removing short oligonucleotides of 3-30 nucleotides from adjacent DNA ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.4 Tequatrovirus T4
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.4 DNA*DNA + H2O 5'-to 3'-exonuclease activity, degradation of DNA*DNA duplexes Tequatrovirus T4 ?
-
 ?
3.1.26.4 additional information regulation Tequatrovirus T4 ?
-
 ?
3.1.26.4 RNA*DNA hybrid + H2O 5'-to 3'-exonuclease activity, degradation of RNA*DNA duplexes Tequatrovirus T4 DNA + 5'-phosphonucleotides
-
 ?
3.1.26.4 RNA-DNA*DNA hybrid + H2O enzyme removes RNA primers from lagging strand fragments during DNA replication, 5'-to 3'-exonuclease activity, degradation of the RNA portion of the duplex Tequatrovirus T4 DNA*DNA + 5'-phosphomononucleotides
-
 ?
3.1.26.4 RNA-DNA*DNA hybrid + H2O specific removal of the RNA portion of the duplex Tequatrovirus T4 DNA*DNA + 5'-phosphomononucleotides
-
 ?
3.1.26.4 ssDNA-dsDNA + H2O 5'-to 3'-exonuclease activity, exonuclease activity removing short oligonucleotides of 3-30 nucleotides from adjacent DNA Tequatrovirus T4 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.26.4 RNase H
-
 Tequatrovirus T4
3.1.26.4 T4 RNase H
-
 Tequatrovirus T4

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.26.4 30
-
 assay at Tequatrovirus T4

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.26.4 7.5
-
 assay at Tequatrovirus T4