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Literature summary extracted from

  • Mitsutake, S.; Tani, M.; Okino, N.; Mori, K.; Ichinose, S.; Omori, A.; Iida, H.; Nakamura, T.; Ito, M.
    Purification, characterization, molecular cloning, and subcellular distribution of neutral ceramidase of rat kidney (2001), J. Biol. Chem., 276, 26249-26259.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.23 sodium cholate 112 kDa CDase from kidney, optimum concentration: 0.4-2%, 4-5fold activation Rattus norvegicus
3.5.1.23 sodium taurodeoxycholate 112 kDa CDase from kidney, optimum concentration: 0.1-0.2%, 4-5fold activation Rattus norvegicus
3.5.1.23 Triton X-100 2fold activation at 0.1-0.2%, but inhibitory beyond the optimum concentration Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.23 112 kDa-form from kidney, expression in Escherichia coli BL21(DE3), in CHOP cells and overexpression in HEK-239 cells Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.23 Cu2+ 80% inhibition of 112 kDa CDase from kidney Rattus norvegicus
3.5.1.23 Hg2+ complete inhibition of 112 kDa CDase from kidney Rattus norvegicus
3.5.1.23 additional information EDTA, Mn2+ and Mg2+ have little effect on activity of 112 kDa CDase from kidney Rattus norvegicus
3.5.1.23 Triton X-100 2fold activation at 0.1-0.2%, but inhibitory beyond the optimum concentration Rattus norvegicus
3.5.1.23 Zn2+ 80% inhibition of 112 kDa CDase from kidney Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.23 endoplasmic reticulum Myc-tagged CDase expressed in HEK-239 cells, a 113 kDa endoplasmic reticulum-form matures into a 133 kDa CDase during processing in the Golgi apparatus Rattus norvegicus 5783
-
3.5.1.23 endosome CDase is distributed with late endosomes/lysosomes in hepatocytes Rattus norvegicus 5768
-
3.5.1.23 Golgi apparatus Myc-tagged CDase expressed in HEK-239 cells, a 113 kDa endoplasmic reticulum-form matures into a 133 kDa CDase during processing in the Golgi apparatus Rattus norvegicus 5794
-
3.5.1.23 lysosome CDase is distributed with late endosomes/lysosomes in hepatocytes Rattus norvegicus 5764
-
3.5.1.23 membrane membrane-bound, 112 kDa-form, mainly localized at the apical membrane of proximal tubules, distal tubules and collecting ducts of kidney, microsome, sequence contains one possible transmembrane domain Rattus norvegicus 16020
-
3.5.1.23 microsome kidney, microsomal fraction contains a large amount of membrane-bound 112 kDa neutral CDase Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.23 additional information 112 kDa CDase from kidney is not activated by Ca2+ Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.23 83483
-
 x * 112000, membrane-bound CDase from kidney, SDS-PAGE, x * 83483, CDase from kidney, sequence calculation, x * 113000, endoplasmic reticulum-form of Myc-tagged CDase expressed in HEK-239, SDS-PAGE, x * 133000, Golgi-form of Myc-tagged CDase expressed in HEK-239, SDS-PAGE Rattus norvegicus
3.5.1.23 112000
-
 x * 112000, membrane-bound CDase from kidney, SDS-PAGE, x * 83483, CDase from kidney, sequence calculation, x * 113000, endoplasmic reticulum-form of Myc-tagged CDase expressed in HEK-239, SDS-PAGE, x * 133000, Golgi-form of Myc-tagged CDase expressed in HEK-239, SDS-PAGE Rattus norvegicus
3.5.1.23 113000
-
 x * 112000, membrane-bound CDase from kidney, SDS-PAGE, x * 83483, CDase from kidney, sequence calculation, x * 113000, endoplasmic reticulum-form of Myc-tagged CDase expressed in HEK-239, SDS-PAGE, x * 133000, Golgi-form of Myc-tagged CDase expressed in HEK-239, SDS-PAGE Rattus norvegicus
3.5.1.23 133000
-
 x * 112000, membrane-bound CDase from kidney, SDS-PAGE, x * 83483, CDase from kidney, sequence calculation, x * 113000, endoplasmic reticulum-form of Myc-tagged CDase expressed in HEK-239, SDS-PAGE, x * 133000, Golgi-form of Myc-tagged CDase expressed in HEK-239, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.23 Rattus norvegicus Q91XT9
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.23 glycoprotein 112 kDa CDase from kidney, highly N-glycosylated, sequence contains 9 putative N-glycosylation sites, the N-glycosylation process is indispensable for enzyme activity Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.23 112 kDa-form from kidney: 3604fold, completely insoluble by freeze-thawing in contrast to liver CDase Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.23 brain high CDase expression Rattus norvegicus
-
3.5.1.23 heart high CDase expression Rattus norvegicus
-
3.5.1.23 kidney high CDase expression, enzyme is mainly localized at the apical membrane of proximal tubules, distal tubules and collecting ducts, enzyme is enriched in the raft microdomains with cholesterol and GM1 ganglioside Rattus norvegicus
-
3.5.1.23 liver low CDase expression, enzyme is distributed with late endosomes/lysosomes in hepatocytes Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.23 1.91
-
 pH 7.5, 37°C, 112 kDa CDase from kidney, hydrolysis of 4-nitrobenzo-2-oxa-1,3-diazoyl-N-dodecanoylsphingosine Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.23 4-nitrobenzo-2-oxa-1,3-diazoyl-N-dodecanoylsphingosine + H2O much faster hydrolysis than of N-lauroylsphingosine Rattus norvegicus 4-nitrobenzo-2-oxa-1,3-diazoyl-dodecanoic acid + sphingosine
-
 ?
3.5.1.23 additional information not: glycosphingolipids, e.g. GalCer, sulfatide, Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1’)ceramide, sphingomyelin Rattus norvegicus ?
-
 ?
3.5.1.23 N-acylsphingosine + H2O CDase catalyzes the hydrolysis of the N-acyl linkage of ceramide to produce sphingosine Rattus norvegicus a carboxylate + sphingosine
-
 ?
3.5.1.23 N-lauroylsphingosine + H2O more efficient hydrolysis than of N-palmitoylsphingosine and N-stearoylsphingosine, less efficient hydrolysis than of 4-nitrobenzo-2-oxa-1,3-diazoyl-N-dodecanoylsphingosine Rattus norvegicus laurate + sphingosine
-
 ?
3.5.1.23 N-palmitoylsphinganine + H2O low hydrolysis rate Rattus norvegicus palmitate + sphinganine
-
 ?
3.5.1.23 N-palmitoylsphingosine + H2O less efficient hydrolysis than of N-lauroylsphingosine, more efficient hydrolysis than of N-stearoylsphingosine Rattus norvegicus palmitate + sphingosine
-
 ?
3.5.1.23 N-stearoylsphinganine + H2O low hydrolysis rate Rattus norvegicus stearate + sphinganine
-
 ?
3.5.1.23 N-stearoylsphingosine + H2O less efficient hydrolysis than of N-lauroylsphingosine and N-palmitoylsphingosine Rattus norvegicus stearate + sphingosine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.23 ? x * 112000, membrane-bound CDase from kidney, SDS-PAGE, x * 83483, CDase from kidney, sequence calculation, x * 113000, endoplasmic reticulum-form of Myc-tagged CDase expressed in HEK-239, SDS-PAGE, x * 133000, Golgi-form of Myc-tagged CDase expressed in HEK-239, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.5.1.23 CDase
-
 Rattus norvegicus
3.5.1.23 neutral ceramidase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.23 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.23 6 7 112 kDa CDase from kidney Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.23 8 9 112 kDa CDase from kidney, 50% of activity in the range Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.1.23 Rattus norvegicus 112 kDa CDase from kidney, sequence calculation
-
 7.1