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Literature summary extracted from

  • Oyedotun, K.S.; Lemire, B.D.
    The Quinone-binding sites of the Saccharomyces cerevisiae succinate-ubiquinone oxidoreductase (2001), J. Biol. Chem., 276, 16936-16943.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.3.5.1 F69V mutation in anchor subunit Sdh4, 56% of wild-type kcat Saccharomyces cerevisiae
1.3.5.1 H99L mutation in anchor subunit Sdh4, 29% of wild-type kcat Saccharomyces cerevisiae
1.3.5.1 S71A mutation in anchor subunit Sdh4, 43% of wild-type kcat Saccharomyces cerevisiae
1.3.5.1 Y89OCH substitution with stop codon truncates Sdh4 by removing the third predicted transmembrane segment, 22% of wild-type activity Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.5.1 2-sec-butyl-4,6-dinitrophenol non-competitive inhibition Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.5.1 0.0037
-
 2,3-Dimethoxy-5-methyl-6-decyl-1,4-benzoquinone 22°C Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.5.1 membrane
-
 Saccharomyces cerevisiae 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.5.1 succinate + ubiquinone Saccharomyces cerevisiae
-
 fumarate + ubiquinol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.1 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.1 succinate + 2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone
-
 Saccharomyces cerevisiae fumarate + 2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinol
-
 ?
1.3.5.1 succinate + ubiquinone
-
 Saccharomyces cerevisiae fumarate + ubiquinol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.5.1 complex II
-
 Saccharomyces cerevisiae
1.3.5.1 SDH
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.5.1 40
-
 no loss of activity after 10 min Saccharomyces cerevisiae
1.3.5.1 65
-
 almost complete loss of activity after 10 min Saccharomyces cerevisiae
1.3.5.1 68
-
 approx. 50% loss of activity after 10 min Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.5.1 3.6
-
 2,3-Dimethoxy-5-methyl-6-decyl-1,4-benzoquinone 22°C Saccharomyces cerevisiae